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Home / Equine Research Bank / Parasitol Res / Rapid detection and identification of Theileria equi and Bab...
Parasitology research2013; 112(11); 3883-3886; doi: 10.1007/s00436-013-3581-2

Rapid detection and identification of Theileria equi and Babesia caballi by high-resolution melting (HRM) analysis.

Abstract: The application of high-resolution melting (HRM) analysis in the differentiation between Theileria equi and Babesia caballi was evaluated using control samples from the United States Department of Agriculture and field samples collected from horses in Sudan and China. A region of the 18S rRNA gene, with four known nucleotide differences between the two parasites, was selected for primer design. HRM analysis successfully allowed the detection and differentiation of T. equi and B. caballi without the necessity of performing time-consuming and expensive post-PCR procedures such as sequencing or restriction digestion. Our results suggest that HRM could be an ideal method for rapid genotyping, which is required to determine a drug of choice or to administer an appropriate vaccine during an outbreak.
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Publication Date: 2013-08-29 PubMed ID: 23990047DOI: 10.1007/s00436-013-3581-2Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study examines the capability of high-resolution melting (HRM) analysis in distinguishing between two types of parasites — Theileria equi and Babesia caballi, found in horses, providing a more time-efficient and cost-effective method than complex post-PCR options like sequencing or restriction digestion.

Introduction to the Research

  • The focus of the research is to explore whether high-resolution melting (HRM) analysis can be effectively used to differentiate between the parasites Theileria equi, and Babesia caballi.
  • HRM analysis is a method used in molecular biology for detecting variations in genetic sequences. It has potential for providing a quicker and less expensive alternative to other methods, such as sequencing or restriction digestion, which are current post-PCR (Polymerase chain reaction) procedures.

The Methodology of the Study

  • The researchers picked a region of the 18S rRNA gene wherein four known nucleotide differences between these two parasites exist for primer design.
  • The evaluation of this was carried out using control samples from the United States Department of Agriculture and field samples from horses in Sudan and China.

Results of the Study

  • The use of HRM analysis proved successful. It allowed for the detection as well as differentiation of Theileria equi and Babesia caballi.
  • This proved that HRM analysis holds potential to be a less time-consuming and cheaper alternative to the currently used post-PCR procedures like sequencing or restriction digestion, without compromising on accuracy.

Significance of the Findings

  • The results of the study suggest that HRM can be an ideal method for rapid genotyping. This rapid genotyping can be instrumental in selecting an appropriate drug or vaccine during an outbreak quickly.
  • These findings indicate potential significant implications in veterinary medicine, particularly in the treatment of livestock infections, where rapid identification and treatment are crucial.

Cite This Article

APA
Salim B, Bakheit MA, Sugimoto C. (2013). Rapid detection and identification of Theileria equi and Babesia caballi by high-resolution melting (HRM) analysis. Parasitol Res, 112(11), 3883-3886. https://doi.org/10.1007/s00436-013-3581-2

Publication

Parasitology research
ISSN: 1432-1955
NlmUniqueID: 8703571
Country: Germany
Language: English
Volume: 112
Issue: 11
Pages: 3883-3886

Researcher Affiliations

Salim, Bashir
  • Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, 13314, Khartoum North, Sudan, bashirsalim@gmail.com.
Bakheit, Mohammed Ahmed
    Sugimoto, Chihiro

      MeSH Terms

      • Animals
      • Babesia / classification
      • Babesia / genetics
      • Babesia / isolation & purification
      • China
      • DNA, Protozoan / genetics
      • DNA, Ribosomal / genetics
      • Genotype
      • Horses / parasitology
      • Molecular Diagnostic Techniques / methods
      • Parasitology / methods
      • RNA, Ribosomal, 18S / genetics
      • Sudan
      • Theileria / classification
      • Theileria / genetics
      • Theileria / isolation & purification
      • Transition Temperature
      • United States

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      Citations

      This article has been cited 6 times.
      1. Wang Y, Zhang S, Li X, Nian Y, Liu X, Liu J, Yin H, Guan G, Wang J. A high-resolution melting approach for the simultaneous differentiation of five human babesiosis-causing Babesia species. Parasit Vectors 2023 Aug 28;16(1):299.
        doi: 10.1186/s13071-023-05839-5pubmed: 37641091google scholar: lookup
      2. Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny. Pathogens 2020 Nov 8;9(11).
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        doi: 10.1186/s13071-019-3781-4pubmed: 31694676google scholar: lookup
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