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Rapid species identification of group C streptococci isolated from horses.
Abstract: Two commercial systems, the API 20S (Analytab Products, Plainview, N.Y.) and the Rapid Strep (API System S.A., Montalieu-Vercieu, France), were evaluated for ease of use and accuracy in the rapid identification of group C streptococci isolated from horses. A total of 85 Streptococcus isolates were tested, including S. equi (67 isolates), S. zooepidemicus (13 isolates), and S. equisimilis (5 isolates). All S. equi and S. zooepidemicus isolates were correctly identified within 24 h by the Rapid Strep system. Specific grouping sera was necessary to distinguish between S. equisimilis and group G or L strains. The API 20S system did not provide species identification of any of these isolates. An identification of randomly selected isolates to species level was performed by conventional methods and confirmed the identification derived through the Rapid Strep system. Our results indicate that the Rapid Strep system is a valuable aid for species identification of equine isolates of group C streptococci.
Publication Date: 1985-04-01 PubMed ID: 3988897PubMed Central: PMC271711DOI: 10.1128/jcm.21.4.524-526.1985Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article evaluates the accuracy and ease of use of two commercial systems, API 20S and Rapid Strep, in quickly identifying group C streptococci found in horses. Their study demonstrates that the Rapid Strep system is effective in correctly identifying the streptococci species within 24 hours, whereas the API 20S system is not.
Testing Process
- The authors tested a total of 85 Streptococcus isolates which were divided into three types: S. equi (67 isolates), S. zooepidemicus (13 isolates), and S. equisimilis (5 isolates).
- They recorded the results from both systems, analyzing the speed and accuracy of each one.
Rapid Strep System
- All S. equi and S. zooepidemicus isolates were correctly identified within 24 hours by the Rapid Strep system.
- To distinguish between S. equisimilis and group G or L strains, it was found necessary to use specific grouping sera.
- This indicates that the Rapid Strep system has the potential to be a very valuable tool for species identification of equine isolates of group C streptococci.
API 20S System
- However, the API 20S system did not provide species identification of any of these isolates.
- As a result, this system was deemed ineffective for the rapid detection of different species of group C streptococci in horses.
Validation with Conventional Methods
- To confirm the accuracy of the Rapid Strep system, the authors performed identification of randomly selected isolates to species level using conventional methods.
- This validation step confirmed the identification derived through the Rapid Strep system, reinforcing its effectiveness.
Cite This Article
APA
Bannister MF, Benson CE, Sweeney CR.
(1985).
Rapid species identification of group C streptococci isolated from horses.
J Clin Microbiol, 21(4), 524-526.
https://doi.org/10.1128/jcm.21.4.524-526.1985 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Fermentation
- Horses / microbiology
- Indicators and Reagents
- Reagent Strips
- Streptococcus / isolation & purification
- Streptococcus / metabolism
References
This article includes 4 references
- Fallon EH. The clinical aspects of streptococcic infections of horses.. J Am Vet Med Assoc 1969 Jul 15;155(2):413-4.
- Proctor DL. Comments on streptococcic infections.. J Am Vet Med Assoc 1969 Jul 15;155(2):414-5.
- Poutrel B, Ryniewicz HZ. Evaluation of the API 20 Strep system for species identification of streptococci isolated from bovine mastitis.. J Clin Microbiol 1984 Feb;19(2):213-4.
- Facklam RR, Rhoden DL, Smith PB. Evaluation of the Rapid Strep system for the identification of clinical isolates of Streptococcus species.. J Clin Microbiol 1984 Nov;20(5):894-8.
Citations
This article has been cited 11 times.- Garner C, Stephen C, Pant SD, Ghorashi SA. Comparison of PCR-HRM, colorimetric LAMP and culture based diagnostic assays in the detection of endometritis caused by Streptococcus equi subsp. zooepidemicus in mares. Vet Res Commun 2023 Jun;47(2):495-509.
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- Kinoshita Y, Niwa H, Katayama Y. Development of a loop-mediated isothermal amplification method for detecting Streptococcus equi subsp. zooepidemicus and analysis of its use with three simple methods of extracting DNA from equine respiratory tract specimens. J Vet Med Sci 2014 Sep;76(9):1271-5.
- Webb K, Barker C, Harrison T, Heather Z, Steward KF, Robinson C, Newton JR, Waller AS. Detection of Streptococcus equi subspecies equi using a triplex qPCR assay. Vet J 2013 Mar;195(3):300-4.
- Holden MT, Heather Z, Paillot R, Steward KF, Webb K, Ainslie F, Jourdan T, Bason NC, Holroyd NE, Mungall K, Quail MA, Sanders M, Simmonds M, Willey D, Brooks K, Aanensen DM, Spratt BG, Jolley KA, Maiden MC, Kehoe M, Chanter N, Bentley SD, Robinson C, Maskell DJ, Parkhill J, Waller AS. Genomic evidence for the evolution of Streptococcus equi: host restriction, increased virulence, and genetic exchange with human pathogens. PLoS Pathog 2009 Mar;5(3):e1000346.
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- Inzana TJ, Iritani B. Rapid detection of group C streptococci from animals by latex agglutination. J Clin Microbiol 1989 Feb;27(2):309-12.
- Seeger MG, de Vargas APC, Vogel FSF, Cargnelutti JF. Streptococcus equi subsp. equi isolated from horses in Southern Brazil: molecular and phenotypic analyses. Braz J Microbiol 2025 Dec;56(4):2987-2996.
- Zu H, Sun R, Li J, Guo X, Wang M, Guo W, Wang X. Integrated CRISPR-Cas12a and RAA one-pot visual strategy for the rapid identification of Streptococcus equi subspecies equi. Front Cell Infect Microbiol 2025;15:1526516.
- Frymus T, Addie DD, Boucraut-Baralon C, Egberink H, Gruffydd-Jones T, Hartmann K, Horzinek MC, Hosie MJ, Lloret A, Lutz H, Marsilio F, Pennisi MG, Radford AD, Thiry E, Truyen U, Möstl K. Streptococcal infections in cats: ABCD guidelines on prevention and management. J Feline Med Surg 2015 Jul;17(7):620-5.
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