Relationship Between Neutrophil Activity, Oxidative Stress, Acute Phase Response, and Lameness Grade in Naturally Occurring Acute and Chronic Joint Disorders in Horses.
Abstract: We hypothesized that in horses with naturally occurring joint disorders, the neutrophil response, acute phase response (APR), and oxidative stress parameters elevated significantly and are markers of increased inflammatory response in these conditions. Therefore, the first aim of the study was to evaluate neutrophil response, oxidative status, and APR. The neutrophil activity was assessed on the basis of elastase, myeloperoxidase (MPO), and alkaline phosphatase release, whereas free radical generation was assessed on the basis of nitric oxide and superoxide production. Acute phase response was estimated on the basis of fibrinogen or haptoglobin plasma concentration and oxidative stress on the basis of malondialdehyde plasma concentration. Then, these parameters were compared with lameness grade, and correlation coefficients between them were calculated. The study was conducted on 43 horses divided on control group of healthy horses (n = 17), acute lameness (AL) group (11 horses), and chronic lameness (CL) group (15 horses). The neutrophil activity from horses of both groups of joint disorders (AL and CL) was significantly (P < .01) higher in comparison with healthy horses. Elastase release was 67.28 ± 1.89% of maximal activity in AL group in comparison with 51.72 ± 1.75% in healthy horses and 62.61 ± 1.54% in CL group. The highest values of other enzymes were also noted in AL group. Moreover, in AL group release of elastase and MPO positively correlated (P < .01) with grades of lameness. These findings revealed the mutual relation between studied parameters and obtained results may be useful in the development of new therapeutic strategies in the treatment of acute and chronic joint disorders in horses.
Copyright © 2020 Elsevier Inc. All rights reserved.
Publication Date: 2020-03-02 PubMed ID: 32303320DOI: 10.1016/j.jevs.2020.102972Google Scholar: Lookup
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Summary
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The research article investigates how neutrophil response, acute phase response (APR), and oxidative stress parameters behave in horses affected by joint disorders. The study found that all these parameters increased significantly in horses with joint disorders, suggesting heightened inflammatory response in such conditions. The parameters were compared with the severity of lameness in the horses, showing a positive correlation.
Study Objectives and Methods
- The main objective of the study was to evaluate the level of neutrophil response, oxidative stress, and APR in horses with joint disorders.
- Neutrophil activity was measured on the basis of elastase, myeloperoxidase (MPO), and alkaline phosphatase release.
- Free radical generation was assessed based on nitric oxide and superoxide production.
- The APR was estimated on the basis of plasma concentration of fibrinogen or haptoglobin and oxidative stress was determined by the malondialdehyde plasma concentration.
- The parameters above were then cross-checked against the grade of lameness in the horses and a correlation was calculated.
Test Groups and Results
- The study involved 43 horses in total: 17 healthy horses (control group), 11 with acute lameness (AL group) and 15 with chronic lameness (CL group).
- The results showed that neutrophil activity was significantly higher (P < .01) in the AL and CL groups compared to the healthy group.
- The elastase release was highest in the AL group (67.28 ± 1.89% of maximal activity), compared to the healthy horses (51.72 ± 1.75%) and the CL group (62.61 ± 1.54%).
- Other enzymes also showed their highest values in the AL group.
- Additionally, the release of elastase and MPO showed a positive correlation with the grades of lameness (P < .01) in the AL group.
Implications of the Study
- The results unveiling the relationship between the parameters studied could be valuable in devising new therapeutic strategies for the treatment of acute and chronic joint disorders in horses.
Cite This Article
APA
Michalska J, Nowicka B, Wessely-Szponder J.
(2020).
Relationship Between Neutrophil Activity, Oxidative Stress, Acute Phase Response, and Lameness Grade in Naturally Occurring Acute and Chronic Joint Disorders in Horses.
J Equine Vet Sci, 88, 102972.
https://doi.org/10.1016/j.jevs.2020.102972 Publication
Researcher Affiliations
- Faculty of Veterinary Medicine, Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, University of Life Sciences, Lublin, Poland. Electronic address: joanna.michalska15@gmail.com.
- Faculty of Veterinary Medicine, Department and Clinic of Animal Surgery, University of Life Sciences, Lublin, Poland.
- Faculty of Veterinary Medicine, Sub-Department of Pathophysiology, Department of Preclinical Veterinary Sciences, University of Life Sciences, Lublin, Poland.
MeSH Terms
- Acute-Phase Reaction / veterinary
- Animals
- Gait
- Horse Diseases
- Horses
- Neutrophils
- Oxidative Stress
Citations
This article has been cited 2 times.- Costa GL, Tabbì M, Bruschetta G, Spadola F, Leonardi F, Bruno F, Iannelli NM, Licata P, Macrì F, Passino ES, Macrì D, Interlandi C. Analgesic efficacy of tapentadol in chronic joint disorders in horses: plasma serotonin concentration and adrenocortical response as biomarkers of pain-induced stress. Front Vet Sci 2024;11:1505398.
- Aragona F, Tabbì M, Gugliandolo E, Giannetto C, D'Angelo F, Fazio F, Interlandi C. Role of cannabidiolic acid or the combination of cannabigerol/cannabidiol in pain modulation and welfare improvement in horses with chronic osteoarthritis. Front Vet Sci 2024;11:1496473.
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