Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line.
Abstract: Equine herpesvirus type 1 (EHV-1) causes respiratory infections, abortion and neurological disorders in horses. Molecular epidemiology studies have demonstrated that a single-point mutation in DNA polymerase gene, resulting in an amino acid variation (N752/D752), is significantly associated with the neuropathogenic potential of EHV-1 strains. The aim of the study was to elucidate if there are any differences between neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains in their ability to infect neuronal cells. For the tested EHV-1 strains, cytopathic effect (CPE) was manifested by changed morphology of cells, destruction of actin cytoskeleton and nuclei degeneration, which led to focal degeneration. Moreover, EHV-1 26 strain caused fusion of the infected cells to form syncytia in culture. Real-time PCR analysis demonstrated that both neuropathogenic and non-neuropathogenic EHV-1 strains replicated in neurons and ED cells (equine dermal cell line) at a similar level. We can assume that a point mutation in the EHV-1 polymerase does not affect viral replication in this cell type.
Publication Date: 2017-01-17 PubMed ID: 28092604DOI: 10.1515/pjvs-2016-0098Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article examines the different effects and replication rates of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in neuronal cells, aiming to determine if a specific mutation in the viral DNA influences the virus’s behavior.
Background and Aim
- The research was driven by the awareness that EHV-1 causes several issues in horses including respiratory infections, abortion, and neurological disorders. Moreover, past research revealed a correlation between a single-point mutation in the DNA polymerase gene of the virus and its neuropathogenic potential.
- The primary objective of the study was to identify any variance in the ability of neuropathogenic (EHV-1 26) and non-neuropathogenic (Jan-E and Rac-H) EHV-1 strains to infect neuronal cells.
Method & Findings
- The team observed cytopathic effects (CPE) caused by the EHV-1 strains. These included changes in cell morphology, destruction of the actin cytoskeleton, and nuclei degeneration, all of which resulted in focal degeneration.
- It was notable that the EHV-1 26 strain specifically in the culture system caused fusion of affected cells leading to the formation of syncytia (a mass of cytoplasm containing several nuclei and enclosed in a membrane but no internal cell boundaries).
- They employed real-time PCR analysis, a technique that allowed them to monitor the progress of amplification in “real-time”, to examine the replication rates of both the neuropathogenic and non-neuropathogenic EHV-1 strains.
- This analysis showed that both strains of EHV-1 displayed similar replication rates within neurons and ED cells (equine dermal cell line).
Conclusion
- Based on the results, the study suggested that the point mutation in the EHV-1 polymerase gene observed with the neuropathogenic strain does not influence its rate of replication within neurons and ED cells. This indicates that other factors may be involved in the virus’s neuropathogenic potential.
Cite This Article
APA
Cymerys J, Słońska A, Brzezicka J, Tucholska A, Chmielewska A, Rola J, Malik P, Bańbura MW.
(2017).
Replication kinetics of neuropathogenic and non-neuropathogenic equine herpesvirus type 1 (EHV-1) strains in primary murine neurons and ED cell line.
Pol J Vet Sci, 19(4), 777-784.
https://doi.org/10.1515/pjvs-2016-0098 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cell Line
- Fibroblasts / virology
- Herpesvirus 1, Equid / physiology
- Horses
- Mice
- Neurons / virology
- Time Factors
- Virus Replication / physiology
Citations
This article has been cited 3 times.- Chodkowski M, Słońska A, Gregorczyk-Zboroch K, Nowak-Zyczynska Z, Golke A, Krzyżowska M, Bańbura MW, Cymerys J. Equid Alphaherpesvirus 1 (EHV-1) Influences Morphology and Function of Neuronal Mitochondria In Vitro. Pathogens 2022 Aug 3;11(8).
- Bartak M, Chodkowski M, Słońska A, Grodzik M, Szczepaniak J, Bańbura MW, Cymerys J. Equid Alphaherpesvirus 1 Modulates Actin Cytoskeleton and Inhibits Migration of Glioblastoma Multiforme Cell Line A172. Pathogens 2022 Mar 25;11(4).
- Słońska A, Cymerys J. Equid alphaherpesvirus 1 (EHV-1) infection of primary murine astrocytes: role of the cytoskeleton. Arch Virol 2025 Dec 11;171(1):19.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
