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Home / Equine Research Bank / Vet Parasitol / Resistance to pyrantel embonate and efficacy of fenbendazole...
Veterinary parasitology2018; 264; 69-73; doi: 10.1016/j.vetpar.2018.11.003

Resistance to pyrantel embonate and efficacy of fenbendazole in Parascaris univalens on Swedish stud farms.

Abstract: The aims of this study were to determine the species of Parascaris present in foals in Sweden and to establish whether anthelmintic resistance to pyrantel and fenbendazole is present on Swedish stud farms. Ascarid eggs collected from different regions in Sweden were karyotyped and were all identified as Parascaris univalens, characterized by one chromosomal pair. Faecal egg count reduction tests were performed on a total of 142 foals on 9 farms between September 2016 and May 2017. Healthy foals with at least 150 eggs per gram faeces (EPG) were included in the study and treated with oral pastes of pyrantel embonate or fenbendazole according to manufacturer instructions. The efficacy of the drugs was calculated by a Bayesian model using the R package "eggCounts". In accordance with the American Association of Equine Practitioners, parasites were classified as resistant to pyrantel if the reduction in EPG was ≤ 85% and to fenbendazole if the observed efficacy was ≤ 90%. Four of eleven groups treated with pyrantel had an observed efficacy of ≤ 85%, and as many as 43% of the foals treated with pyrantel excreted eggs 10-16 days after treatment. In contrast, one of the six groups treated with fenbendazole had an observed efficacy of ≤ 90%, and only 6% of all foals were excreting eggs 10-16 days after treatment. Since resistance to ivermectin has earlier been shown to be widespread in Parascaris spp. in Sweden it is likely that multiresistant populations are present on Swedish stud farms. This is the first study showing the existence of pyrantel-resistant Parascaris spp. in Europe, and the first ever study where anthelmintic resistance has been shown in P. univalens.
Copyright © 2018 The Author(s). Published by Elsevier B.V. All rights reserved.
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Publication Date: 2018-11-10 PubMed ID: 30503095DOI: 10.1016/j.vetpar.2018.11.003Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study focuses on the resistance of the Parascaris univalens species to certain parasitic drugs, specifically pyrantel and fenbendazole, on Swedish stud farms. The research finds that there is evidence of such resistance emerging, posing a potential problem for equine health.

Objective and Methods

  • The research had two main goals: Identifying the species of Parascaris present among foals in Sweden and determining whether these species display resistance to two anthelmintic drugs, pyrantel and fenbendazole.
  • Ascarid eggs from various regions in Sweden were collected and identified as Parascaris univalens, a species with a single chromosomal pair.
  • The researchers conducted faecal egg count reduction tests on 142 foals from nine farms from September 2016 to May 2017, to evaluate the efficacy of the two drugs.
  • Foals healthy enough to have at least 150 eggs per gram faeces (EPG) were included in the study and given oral pastes of either pyrantel embonate or fenbendazole per manufacturer guidelines.
  • A Bayesian model, using the R package “eggCounts”, was employed to compute drug efficacies.

Findings

  • According to the American Association of Equine Practitioners, parasites resistant to pyrantel would show a reduction in EPG of ≤ 85%, and to fenbendazole if efficacy was ≤ 90%.
  • Out of the eleven groups treated with pyrantel, four showed an efficacy of ≤ 85% indicating resistance.
  • Furthermore, up to 43% of the foals treated with pyrantel were still excreting eggs 10-16 days following treatment, again suggesting resistance.
  • Contrastingly, only one out of the six groups treated with fenbendazole showed an efficacy of ≤ 90%, indicating less resistance.
  • Moreover, only 6% of non-resisting foals were still excreting eggs 10-16 days after treatment with fenbendazole.
  • The research also points to the potential of multiresistant populations on Swedish stud farms due to prior findings of ivermectin resistance among Parascaris spp.

Significance

  • This study is the first of its kind to record the existence of pyrantel-resistant Parascaris spp. in Europe and the first to demonstrate anthelmintic resistance in P. univalens.

Implications

  • The rising resistance to these commonly used drugs implies a growing threat to equine health, especially since multi-resistant populations may already exist.
  • More studies are needed to ascertain the extent of this potential resistance and to develop new effective treatments.

Cite This Article

APA
Martin F, Höglund J, Bergström TF, Karlsson Lindsjö O, Tydén E. (2018). Resistance to pyrantel embonate and efficacy of fenbendazole in Parascaris univalens on Swedish stud farms. Vet Parasitol, 264, 69-73. https://doi.org/10.1016/j.vetpar.2018.11.003

Publication

Veterinary parasitology
ISSN: 1873-2550
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 264
Pages: 69-73

Researcher Affiliations

Martin, Frida
  • Swedish University of Agricultural Sciences, Department of Biomedical Sciences and Veterinary Public Health, Division of Parasitology, Uppsala, Sweden. Electronic address: Frida.martin@slu.se.
Höglund, Johan
  • Swedish University of Agricultural Sciences, Department of Biomedical Sciences and Veterinary Public Health, Division of Parasitology, Uppsala, Sweden.
Bergström, Tomas F
  • Swedish University of Agricultural Sciences, Department of Animal Genetics and Breeding, Uppsala, Sweden.
Karlsson Lindsjö, Oskar
  • Swedish University of Agricultural Sciences, Department of Molecular Sciences, Uppsala, Sweden.
Tydén, Eva
  • Swedish University of Agricultural Sciences, Department of Biomedical Sciences and Veterinary Public Health, Division of Parasitology, Uppsala, Sweden.

MeSH Terms

  • Animals
  • Antinematodal Agents / pharmacology
  • Antinematodal Agents / therapeutic use
  • Ascaridida Infections / drug therapy
  • Ascaridida Infections / parasitology
  • Ascaridida Infections / veterinary
  • Ascaridoidea / drug effects
  • Drug Resistance / drug effects
  • Fenbendazole / therapeutic use
  • Horse Diseases / drug therapy
  • Horse Diseases / parasitology
  • Horses
  • Pyrantel Pamoate / pharmacology
  • Pyrantel Pamoate / therapeutic use
  • Sweden
  • Treatment Outcome

Citations

This article has been cited 19 times.
  1. Nielsen MK, Pyatt A, Perrett J, Tydén E, van Doorn D, Pihl TH, Schmidt JS, von Samson-Himmelstjerna G, Beasley A, Abbas G, Jabbar A. Global equine parasite control guidelines: Consensus or confusion?. Int J Parasitol Drugs Drug Resist 2025 Aug;28:100600.
    doi: 10.1016/j.ijpddr.2025.100600pubmed: 40472642google scholar: lookup
  2. Wolstenholme AJ, Andersen EC, Choudhary S, Ebner F, Hartmann S, Holden-Dye L, Kashyap SS, Krücken J, Martin RJ, Midha A, Nejsum P, Neveu C, Robertson AP, von Samson-Himmelstjerna G, Walker R, Wang J, Whitehead BJ, Williams PDE. Getting around the roundworms: Identifying knowledge gaps and research priorities for the ascarids. Adv Parasitol 2024;123:51-123.
    doi: 10.1016/bs.apar.2023.12.002pubmed: 38448148google scholar: lookup
  3. Zhou M, Lu Y, Han L, Lu M, Guan C, Yu J, Liu H, Chen D, Li H, Yang Y, Zhang L, Tian L, Liu Q, Hou Z. Exploration of Parascaris species in three different Equus populations in China. Parasit Vectors 2023 Jun 15;16(1):202.
    doi: 10.1186/s13071-023-05768-3pubmed: 37322493google scholar: lookup
  4. Buono F, Veneziano V, Veronesi F, Molento MB. Horse and donkey parasitology: differences and analogies for a correct diagnostic and management of major helminth infections. Parasitology 2023 Oct;150(12):1119-1138.
    doi: 10.1017/S0031182023000525pubmed: 37221816google scholar: lookup
  5. Lu Y, Deng L, Peng Z, Zhou M, Wang C, Han L, Huang S, Wei M, Wei R, Tian L, Li D, Hou Z. Investigation of the Efficacy of Pyrantel Pamoate, Mebendazole, Albendazole, and Ivermectin against Baylisascaris schroederi in Captive Giant Pandas. Animals (Basel) 2022 Dec 29;13(1).
    doi: 10.3390/ani13010142pubmed: 36611749google scholar: lookup
  6. Nielsen MK. Anthelmintic resistance in equine nematodes: Current status and emerging trends. Int J Parasitol Drugs Drug Resist 2022 Dec;20:76-88.
    doi: 10.1016/j.ijpddr.2022.10.005pubmed: 36342004google scholar: lookup
  7. Cain JL, Norris JK, Ripley NE, Suri P, Finnerty CA, Gravatte HS, Nielsen MK. The microbial community associated with Parascaris spp. infecting juvenile horses. Parasit Vectors 2022 Nov 4;15(1):408.
    doi: 10.1186/s13071-022-05533-ypubmed: 36333754google scholar: lookup
  8. Cain JL, Nielsen MK. The equine ascarids: resuscitating historic model organisms for modern purposes. Parasitol Res 2022 Oct;121(10):2775-2791.
    doi: 10.1007/s00436-022-07627-zpubmed: 35986167google scholar: lookup
  9. Dube F, Hinas A, Roy S, Martin F, Åbrink M, Svärd S, Tydén E. Ivermectin-induced gene expression changes in adult Parascaris univalens and Caenorhabditis elegans: a comparative approach to study anthelminthic metabolism and resistance in vitro. Parasit Vectors 2022 May 5;15(1):158.
    doi: 10.1186/s13071-022-05260-4pubmed: 35513885google scholar: lookup
  10. Lamassiaude N, Courtot E, Corset A, Charvet CL, Neveu C. Pharmacological characterization of novel heteromeric GluCl subtypes from Caenorhabditis elegans and parasitic nematodes. Br J Pharmacol 2022 Mar;179(6):1264-1279.
    doi: 10.1111/bph.15703pubmed: 34623639google scholar: lookup
  11. Martin F, Halvarsson P, Delhomme N, Höglund J, Tydén E. Exploring the β-tubulin gene family in a benzimidazole-resistant Parascaris univalens population. Int J Parasitol Drugs Drug Resist 2021 Dec;17:84-91.
    doi: 10.1016/j.ijpddr.2021.08.004pubmed: 34467878google scholar: lookup
  12. Trailovic SM, Rajkovic M, Marjanovic DS, Neveu C, Charvet CL. Action of Carvacrol on Parascaris sp. and Antagonistic Effect on Nicotinic Acetylcholine Receptors. Pharmaceuticals (Basel) 2021 May 26;14(6).
    doi: 10.3390/ph14060505pubmed: 34073197google scholar: lookup
  13. Scala A, Tamponi C, Sanna G, Predieri G, Meloni L, Knoll S, Sedda G, Dessì G, Cappai MG, Varcasia A. Parascaris spp. eggs in horses of Italy: a large-scale epidemiological analysis of the egg excretion and conditioning factors. Parasit Vectors 2021 May 8;14(1):246.
    doi: 10.1186/s13071-021-04747-wpubmed: 33964977google scholar: lookup
  14. Sazmand A, Bahari A, Papi S, Otranto D. Parasitic diseases of equids in Iran (1931-2020): a literature review. Parasit Vectors 2020 Nov 19;13(1):586.
    doi: 10.1186/s13071-020-04472-wpubmed: 33213507google scholar: lookup
  15. Tydén E, Enemark HL, Franko MA, Höglund J, Osterman-Lind E. Prevalence of Strongylus vulgaris in horses after ten years of prescription usage of anthelmintics in Sweden. Vet Parasitol X 2019 Nov;2:100013.
    doi: 10.1016/j.vpoa.2019.100013pubmed: 32904767google scholar: lookup
  16. Martin F, Dube F, Karlsson Lindsjö O, Eydal M, Höglund J, Bergström TF, Tydén E. Transcriptional responses in Parascaris univalens after in vitro exposure to ivermectin, pyrantel citrate and thiabendazole. Parasit Vectors 2020 Jul 9;13(1):342.
    doi: 10.1186/s13071-020-04212-0pubmed: 32646465google scholar: lookup
  17. Studzińska MB, Sallé G, Roczeń-Karczmarz M, Szczepaniak K, Demkowska-Kutrzepa M, Tomczuk K. A survey of ivermectin resistance in Parascaris species infected foals in south-eastern Poland. Acta Vet Scand 2020 Jun 5;62(1):28.
    doi: 10.1186/s13028-020-00526-2pubmed: 32503589google scholar: lookup
  18. Scare JA, Dini P, Norris JK, Steuer AE, Scoggin K, Gravatte HS, Howe DK, Slusarewicz P, Nielsen MK. Ascarids exposed: a method for in vitro drug exposure and gene expression analysis of anthelmintic naïve Parascaris spp. Parasitology 2020 May;147(6):659-666.
    doi: 10.1017/S0031182020000189pubmed: 32046800google scholar: lookup
  19. Tydén E, Jansson A, Ringmark S. Parasites in Horses Kept in A 2.5 Year-Round Grazing System in Nordic Conditions without Supplementary Feeding. Animals (Basel) 2019 Dec 17;9(12).
    doi: 10.3390/ani9121156pubmed: 31861066google scholar: lookup
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