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Home / Equine Research Bank / Mol Cell Probes / Reverse transcription real-time PCR assays for detection and...
Molecular and cellular probes2006; 21(1); 47-55; doi: 10.1016/j.mcp.2006.08.001

Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts.

Abstract: Borna disease is a severe, immunopathological disorder of the central nervous system caused by the infection with the Borna disease virus (BDV). The detection of BDV in diseased animals, mainly sheep and horses, is achieved by histological, immunohistochemical and serological approaches and/or PCR-based technologies. In the present study, reverse transcription, real-time PCR assays were established for the detection of BDV in the brain tissue from sheep and horses, using loci for the p40 (nucleoprotein) and the p24 (phosphoprotein) genes. The PCRs were equally specific and sensitive, detecting 10 target molecules per reaction and one BDV-infected cell among 10(6) non-infected cells. In tissues from BDV-diseased sheep and horses, the p24 target was detected at higher abundance than for p40. Therefore, the p24 test is suggested to be of higher value in the diagnostic laboratory. However, both assays should be useful for addressing questions in pathogenesis and for detecting BDV reservoirs in endemic areas.
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Publication Date: 2006-08-30 PubMed ID: 17014984PubMed Central: PMC7127217DOI: 10.1016/j.mcp.2006.08.001Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study focuses on the development and application of reverse transcription real-time PCR assays to detect and quantify Borna disease virus in affected hosts, particularly sheep and horses.

Context and Objective

  • The research study is based on Borna disease, a severe disorder affecting the central nervous system of hosts. The disease is caused by the Borna Disease Virus (BDV) and it mainly affects animals like horses and sheep.
  • The study’s main objective is to explore new methods of detecting BDV in the hosts. The traditional methods of detection are histological, immunohistochemical, and serological approaches, and/or PCR-based technologies.
  • The researchers have developed reverse transcription real-time PCR assays for this purpose using loci for the p40 (nucleoprotein) and the p24 (phosphoprotein) genes.

Methods and Findings

  • The assays developed for the study were equally specific and sensitive and could detect up to 10 target molecules per reaction. Importantly, they could identify one BDV-infected cell among 1,000,000 non-infected cells.
  • The study found the p24 target was detected in higher abundance compared to the p40 target in the tissues from BDV-diseased sheep and horses.

Conclusions and Implications

  • Based on the results, the study suggests that the p24 test has a higher value in diagnostic laboratories because of the higher abundance detection of p24.
  • The researchers also suggest that both p40 and p24 assays could be beneficial for addressing questions regarding the pathogenesis of the disease, as well as for detecting BDV reservoirs in areas endemic with the disease.

Cite This Article

APA
Schindler AR, Vögtlin A, Hilbe M, Puorger M, Zlinszky K, Ackermann M, Ehrensperger F. (2006). Reverse transcription real-time PCR assays for detection and quantification of Borna disease virus in diseased hosts. Mol Cell Probes, 21(1), 47-55. https://doi.org/10.1016/j.mcp.2006.08.001

Publication

Molecular and cellular probes
ISSN: 0890-8508
NlmUniqueID: 8709751
Country: England
Language: English
Volume: 21
Issue: 1
Pages: 47-55

Researcher Affiliations

Schindler, A R
  • Institute of Veterinary Pathology, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland.
Vögtlin, A
    Hilbe, M
      Puorger, M
        Zlinszky, K
          Ackermann, M
            Ehrensperger, F

              MeSH Terms

              • Animals
              • Borna Disease / virology
              • Borna disease virus / genetics
              • Borna disease virus / isolation & purification
              • Brain / virology
              • Chlorocebus aethiops
              • Dogs
              • Horse Diseases / virology
              • Horses / virology
              • Immunohistochemistry
              • RNA, Viral / analysis
              • RNA, Viral / genetics
              • Reverse Transcriptase Polymerase Chain Reaction / methods
              • Sensitivity and Specificity
              • Sheep / virology
              • Sheep Diseases / virology
              • Swine / virology
              • Vero Cells

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