Rifaximin anti-inflammatory activity on bovine endometrium primary cell cultures: a preliminary study.
Abstract: Rifaximin is an unabsorbed oral antibiotic showing anti-inflammatory properties in human pathologies like irritable bowel syndrome and inflammatory bowel disease. In veterinary medicine, rifaximin is primarily used in the treatment of dermatological diseases in all animal species, in therapy and prophylaxis of mastitis in cows and in the treatment of endometritis in cattle and horses. The aim of this preliminary study was to evaluate the anti-inflammatory properties of rifaximin on primary cell cultures from bovine endometrium in which inflammatory response was induced by Lipopolysaccaride (LPS) treatment. Epithelial and stromal cells were isolated from bovine endometrium and separately incubated for 24 h with 1 μg mL-1 LPS after rifaximin (10, 50 and 100 μmol L-1 ) or dexamethasone (10 μmol L-1 ) pre-treatment for 24 h. Supernatants were collected 24 h after LPS treatment and interleukin (IL)-6 and IL-8 accumulation was measured by ELISA. Rifaximin (10, 50 and 100 μmol L-1 ) dose dependently inhibited the LPS-induced increase in IL-6 and IL-8 in stromal cells, whereas in epithelial cells it was not possible to detect any accumulation of these interleukins. Rifaximin reduced IL-6 and IL-8 production, showing a potential anti-inflammatory effect that opens up to new possibilities for the use of this drug in uterine inflammatory diseases.
© 2018 The Authors. Veterinary Medicine and Science Published by John Wiley & Sons Ltd.
Publication Date: 2018-07-09 PubMed ID: 29984902PubMed Central: PMC6236135DOI: 10.1002/vms3.115Google Scholar: Lookup
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- Journal Article
Summary
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This research explores the anti-inflammatory properties of rifaximin, an oral antibiotic, on cell cultures from the endometrium (inner lining of the uterus) of cows. The findings suggest that rifaximin may have potential uses for treating uterine diseases.
Summary of The Research
- The article is centered on the evaluation of the anti-inflammatory properties of rifaximin – an oral antibiotic – on primary cell cultures derived from the endometrium of cows. The inflammatory response was induced by Lipopolysaccharide (LPS) treatment.
- Rifaximin has shown anti-inflammatory properties in treating human conditions like irritable bowel syndrome and inflammatory bowel disease. In veterinary medicine, it has been primarily used to treat dermatological diseases in various animal species, and issues such as mastitis in cows and endometritis in cattle and horses.
- The researchers aimed to explore new possibilities for the usage of the drug in treating uterine inflammatory diseases.
The Methodology
- In the study, epithelial and stromal cells (different types of cells from the cow’s endometrium) were isolated and incubated separately for 24 hours with 1 μg mL LPS after being pre-treated for 24 hours with various concentrations of rifaximin or with dexamethasone.
- Dexamethasone, an anti-inflammatory and immunosuppressant, was used as a control.
- Following this incubation period, the supernatants (the fluid portion of a sample) were collected after 24 hours of LPS treatment.
Measuring Interleukin
- Interleukin (IL)-6 and IL-8, indicators of inflammation, were then measured in the cell culture supernatants using an ELISA (Enzyme-Linked Immunosorbent Assay, a method to measure the amount of proteins such as cytokines, in a sample).
- Here, IL-6 and IL-8 acted as biomarkers to assess the level of inflammation taking place.
Findings
- The study found that rifaximin dose-dependently inhibited the LPS-induced increase in IL-6 and IL-8 in stromal cells, indicating that the antibiotic has anti-inflammatory effects.
- In epithelial cells, however, no such accumulation of the interleukins was detected.
- The observations suggest that rifaximin could potentially be used to reduce inflammation in uterine diseases for animals, opening up new potential therapeutic applications for this drug.
Cite This Article
APA
Flammini L, Mantelli L, Volpe A, Domenichini G, Di Lecce R, Dondi M, Cantoni AM, Barocelli E, Quintavalla F.
(2018).
Rifaximin anti-inflammatory activity on bovine endometrium primary cell cultures: a preliminary study.
Vet Med Sci, 4(4), 326-332.
https://doi.org/10.1002/vms3.115 Publication
Researcher Affiliations
- Food and Drug Department, University of Parma, Parma, Italy.
- Department of Medical Veterinary Sciences, University of Parma, Parma, Italy.
- Food and Drug Department, University of Parma, Parma, Italy.
- Food and Drug Department, University of Parma, Parma, Italy.
- Department of Medical Veterinary Sciences, University of Parma, Parma, Italy.
- Department of Medical Veterinary Sciences, University of Parma, Parma, Italy.
- Department of Medical Veterinary Sciences, University of Parma, Parma, Italy.
- Food and Drug Department, University of Parma, Parma, Italy.
- Department of Medical Veterinary Sciences, University of Parma, Parma, Italy.
MeSH Terms
- Animals
- Anti-Bacterial Agents / pharmacology
- Cattle
- Cell Survival / drug effects
- Cells, Cultured
- Endometrium / cytology
- Epithelial Cells / drug effects
- Female
- Inflammation / chemically induced
- Inflammation / drug therapy
- Inflammation / veterinary
- Lipopolysaccharides / toxicity
- Rifaximin / pharmacology
References
This article includes 19 references
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