Screening of nematophagous-fungi from fresh faeces of grazing animals and soils.
Abstract: An investigation was undertaken for screening and isolating nematophagous-fungi from the faecal samples of various grazing animals and soils in Malaysia. Total of 111 faeces and 50 soil samples were collected and the samples were cultured on 2% water agar plates. The growth of nematophagous-fungi was stimulated by sprinkling-baiting technique. The conidia of suspected nematophagous-fungi were inoculated on 2% water agar plates. All isolated were maintained on 2% cornmeal agar plates. Verticillium spp., Fusarium spp. and Arthrobotrys spp. were identified from the faecal and soil samples. 62.5% of the faecal samples and 100% of the soil samples were shown to be positive with nematophagous-fungi. This study highlights the present of nematophagous-fungi population in faecal and soil samples. Much study remains to be done to better understanding some fungi especially their mode of action and their predatory behaviour against parasitic nematodes.
Publication Date: 2019-09-01 PubMed ID: 33597490
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- Journal Article
Summary
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The research article focuses on a study aimed at isolating nematophagous fungi present in the fecal samples of various grazing animals and soils in Malaysia.
Overview of the Research
- The research was conducted with the objective of screening and isolating nematophagous fungi from the feces of different grazing animals and soils in Malaysia.
- A total of 111 feces and 50 soil samples were collected for the study.
- Cultures were grown from these samples on 2% water agar plates with the growth of nematophagous fungi encouraged using a sprinkling-baiting technique.
Culturing Process and Isolation of Fungi
- The conidia, or spores, of the suspected nematophagous fungi were then inoculated, meaning they were deliberately introduced, onto 2% water agar plates.
- All isolates, the selected and cultivated microorganisms, were maintained on 2% cornmeal agar plates.
- Through this process, various species of pathogens, including Verticillium, Fusarium, and Arthrobotrys, were identified from the respective fecal and soil samples.
Results of the Research
- From the collected samples, 62.5% of the fecal samples and 100% of the soil samples were positive for nematophagous fungi.
- This result underscores the prevalence of a significant nematophagous fungi population in the faecal and soil samples.
Implication and Future Direction
- Despite the results, the study emphasizes that a lot of research still needs to be done to better understand these fungi.
- Aspects that require further study include the different fungi’s mode of action as well as their predatory behavior against parasitic nematodes—roundworms that can be harmful to both people and animals.
Cite This Article
APA
Farah Haziqah MT, Nur Hikmah AM, Mat Hasan H, Hamdan A, Nik Him NAII.
(2019).
Screening of nematophagous-fungi from fresh faeces of grazing animals and soils.
Trop Biomed, 36(3), 687-693.
Publication
Researcher Affiliations
- School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
- School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
- School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
- School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
- Vector Control Research Unit, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
- School of Biological Sciences, Universiti Sains Malaysia, 11800 USM, Penang, Malaysia.
MeSH Terms
- Animals
- Cattle
- Deer
- Feces / microbiology
- Goats
- Horses
- Malaysia
- Mitosporic Fungi / classification
- Mitosporic Fungi / isolation & purification
- Nematoda
- Pest Control, Biological
- Soil
- Soil Microbiology
Citations
This article has been cited 1 times.- Sander C, Neumann S. Nematophagous fungi as biological control agents of parasitic nematodes in soils of wildlife parks. Int J Parasitol Parasites Wildl 2025 Apr;26:101033.
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