Seminal concentrations of trace elements in various animals and their correlations.

Abstract: To determine the seminal concentrations of copper, zinc, iron, cadmium, lead and nickel in bulls, rams, boars, stallions and foxes and study their correlations. Methods: Semen samples were obtained, digested and analyzed by means of the atomic absorption spectrophotometer. Data were analyzed statistically with the Student's t-test and Scheffe's test using PC programs SAS and Excel. Results: The seminal copper concentration was significantly higher in ram [(2.49+/-0.18) mg/kg] and fox [(2.16+/-0.53) mg/kg] than that in bull [(1.64+/-0.21) mg/kg], boar [(1.64+/-0.28) mg/kg] and stallion (0.86 mg/kg). In boar a significantly higher seminal zinc concentration (171.74+/-65.72) mg/kg] was found in comparison with stallion [(86.20+/-45.88) mg/kg], bull [(83.15+/-61.61) mg/kg], ram [(60.46+/-35.37) mg/kg] and fox (13.09+/-5.22) mg/kg]. The iron concentration in semen was significantly higher in ram [(40.32+/-10.81) mg/kg), bull [(38.04+/-22.07) mg/kg] and fox [(33.16+/-24.36) mg/kg] than that in boar [(16.14+/-10.35) mg/kg] and stallion (12.68 mg/kg). The seminal cadmium concentration was relatively low [(0.05~0.12) mg/kg] in all studied species. The seminal lead concentration was the highest in ram [(0.35+/-0.68) mg/kg], which was much higher than in fox [(0.08+/-0.06) mg/kg], bull [(0.06+/-0.04) mg/kg], stallion [(0.05+/-0.05) mg/kg] and boar [0.02+/-0.03) mg/kg]. The level of seminal nickel was significantly higher in fox [(0.35+/-0.24) mg/kg] and ram [(0.31+/-0.19) mg/kg] in comparison with bull [(0.12+/-0.07) mg/kg] and boar [(0.06+/-0.08) mg/kg]. The concentration of nickel in the semen of stallion was (0.20 +/-0.24) mg/kg. There was a high positive correlation between seminal iron and zinc in bull (r=0.723) and stallion (r=0.723), between cadmium and lead in ram (r=0.976) and boar (r=0.973) and between iron and cadmium (r=0.783) and iron and lead (r=0.791) in boar. A high negative correlation between seminal nickel and copper in ram (r=-0.709) and between seminal copper and lead in fox (r=-0.854) was found. Conclusions: There are significant differences in the concentrations of seminal elements in different animals. There is a high concentration of seminal copper in ram and fox, a high zinc level in boar, a high iron level in bull, ram and fox and a high nickel level in ram and fox.