Seminal plasma proteins as potential biomarkers for sperm motility and velocities.
Abstract: Seminal plasma proteins have important roles in sperm functionality, and different mechanisms including micro-vesicle transport of proteins are involved in the regulation of sperm biology. Due to the role of seminal plasma, we hypothesized that specific proteins present in seminal plasma may be used as discriminant variables with potential to identify stallions producing different quality ejaculates; 10 fertile stallions, with different motility and velocity values (although within normal ranges) were used in this study. Motilities and velocities were studied using computer assisted sperm analysis (CASA), while protein composition of the seminal plasma was studied using UHPLC-MS/MS. Specific proteins were more abundant in samples with poorer percentages of total motility, average path velocity and circular velocity, and were: Secreted phosphoprotein 1, Fructose-bisphosphate aldolase (p = 1,95E-09; q = 0.0005) and Malate dehydrogenase 1 (p = 1,41E-11; q = 0.002), to the contrary samples with better straight-line velocity values were enriched in Glutathione peroxidase (p=0.00013; q=0.04) and Triosephosphate isomerase (p=0.00015; q=0.04).
Copyright © 2021 Elsevier Inc. All rights reserved.
Publication Date: 2021-10-12 PubMed ID: 34656835DOI: 10.1016/j.theriogenology.2021.10.007Google Scholar: Lookup
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- Journal Article
Summary
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The study investigates how specific proteins present in seminal plasma may act as indicators for different qualities of semen in stallions, specifically concerning sperm motility and velocity.
Objective of research
- The research aims to understand the correlation of specific seminal plasma proteins with the functionality of sperm, particularly in aspects of sperm motility and velocity.
- The study looks at how these proteins could be used as biomarkers to identify stallions producing different quality ejaculates.
The Study Sample
- The research was based on a sample of 10 fertile stallions, all of which displayed varying motility and velocity values but still within normal ranges.
Research Methodology
- Sperm motility and velocity were analyzed using computer assisted sperm analysis (CASA).
- The protein composition in the seminal plasma of these samples was studied using Ultra High Performance Liquid Chromatography with Tandem Mass Spectrometry (UHPLC-MS/MS).
Findings of research
- Results indicated that certain proteins were more prominently present in semen with lower percentages of total motility, average path velocity, and circular velocity. These proteins were identified to be Secreted phosphoprotein 1, Fructose-bisphosphate aldolase and Malate dehydrogenase 1.
- In contrast, sperm samples with better straight-line velocity values were significantly rich in two proteins – Glutathione peroxidase and Triosephosphate isomerase.
Implication of research
- The observations suggest that these specific proteins could be used as potential biomarkers for distinguishing sperm quality.
- They could play an integral role in aiding veterinarians and horse breeders to understand the fertility potential of stallions.
Cite This Article
APA
Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, da Silva-Álvarez E, Masot J, Redondo E, Gil MC, Ortega-Ferrusola C, Peña FJ.
(2021).
Seminal plasma proteins as potential biomarkers for sperm motility and velocities.
Theriogenology, 177, 34-41.
https://doi.org/10.1016/j.theriogenology.2021.10.007 Publication
Researcher Affiliations
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain.
- Laboratory of Equine Reproduction and Equine Spermatology, Veterinary Teaching Hospital, University of Extremadura, Cáceres, Spain. Electronic address: fjuanpvega@unex.es.
MeSH Terms
- Animals
- Biomarkers
- Horses
- Male
- Semen
- Seminal Plasma Proteins
- Sperm Motility
- Spermatozoa
- Tandem Mass Spectrometry / veterinary
Citations
This article has been cited 1 times.- Gaitskell-Phillips G, Martín-Cano FE, Ortiz-Rodríguez JM, Silva-Rodríguez A, da Silva-Álvarez E, Gil MC, Ortega-Ferrusola C, Peña FJ. Dataset of the sperm proteome of stallions with different motility.. Data Brief 2022 Dec;45:108578.
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