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Home / Equine Research Bank / J Virol / Sequence variability of Borna disease virus open reading fra...
Journal of virology1996; 70(1); 635-640; doi: 10.1128/JVI.70.1.635-640.1996

Sequence variability of Borna disease virus open reading frame II found in human peripheral blood mononuclear cells.

Abstract: A cDNA fragment of the Borna disease virus (BDV) open reading frame II (ORF-II), which encodes a 24-kDa phosphoprotein (p24 [P protein]), was amplified from total RNA of peripheral blood mononuclear cells (PBMC) from three psychiatric inpatients. The amplified cDNA fragments were cloned, sequenced, and analyzed. A total of 15 clones, 5 from each patient, were studied. Intrapatient divergencies of the BDV ORF-II nucleotide sequence were 4.2 to 7.3%, 4.8 to 7.3%, and 2.8 to 7.1% for the three patients, leading to differences of 7.7 to 14.5%, 10.3 to 17.1%, and 6.0 to 16.2%, respectively, in the deduced amino acid sequence for BDV p24. Interpatient divergencies among the 15 clones were 5.9 to 12.7% at the nucleotide level and 12.8 to 28.2% at the amino acid level. Thus, in p24, BDV in human PBMC of the patients undergoes mutation at high rates in vivo. Additionally, we found that the nucleotide sequence of the 15 human BDV ORF-II cDNA clones differed from those of the horse strains V and He/80-1 by 4.2 to 9.3%. However, comparison of the consensus amino acid sequence deduced from the 15 human clones with those of the horse strains revealed no human-specific amino acid residue, suggesting that the BDV infecting humans may be related to that infecting horses.
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Publication Date: 1996-01-01 PubMed ID: 8523585PubMed Central: PMC189858DOI: 10.1128/JVI.70.1.635-640.1996Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the sequence variability of Borna Disease Virus open reading frame II in the peripheral blood mononuclear cells of psychiatric inpatients, showing high mutation rates of the virus in the human cells.

Research Methodology

  • The researchers sampled peripheral blood mononuclear cells (PBMC) from three psychiatric inpatients.
  • They then extracted total RNA from these cells.
  • A cDNA fragment of Borna Disease Virus (BDV) open reading frame II (ORF-II), which codes for a 24-kDa phosphoprotein—referred to as P protein or p24—was amplified from the extracted RNA.
  • The amplified cDNA fragments were cloned, sequenced, and then analyzed.
  • Each patient yielded 5 clones, making a total of 15 clones that the researchers analyzed.

Results

  • The researchers found intrapatient divergencies of the BDV ORF-II nucleotide sequence between 2.8% and 7.3% among the three patients.
  • This led to differences between 6.0% and 17.1% in the deduced amino acid sequence for the p24 protein.
  • Interpatient divergencies among the 15 clones were between 5.9% and 12.7% at the nucleotide level, and between 12.8% and 28.2% at the amino acid level.
  • The researchers concluded that BDV in human PBMC undergoes mutation at high rates in vivo.

Comparison with Horse Strains

  • The researchers compared the nucleotide sequence of the 15 human BDV ORF-II cDNA clones with those of horse strains V and He/80-1.
  • They found a difference between 4.2% and 9.3%.
  • However, when they compared the consensus amino acid sequence deduced from the 15 human clones with those of the horse strains, they found no human-specific amino acid residue.
  • This suggests that the BDV infecting humans might be related to that infecting horses.

Cite This Article

APA
Kishi M, Arimura Y, Ikuta K, Shoya Y, Lai PK, Kakinuma M. (1996). Sequence variability of Borna disease virus open reading frame II found in human peripheral blood mononuclear cells. J Virol, 70(1), 635-640. https://doi.org/10.1128/JVI.70.1.635-640.1996

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 70
Issue: 1
Pages: 635-640

Researcher Affiliations

Kishi, M
  • Section of Serology, Hokkaido University, Sapporo, Japan.
Arimura, Y
    Ikuta, K
      Shoya, Y
        Lai, P K
          Kakinuma, M

            MeSH Terms

            • Amino Acid Sequence
            • Animals
            • Base Sequence
            • Borna disease virus / genetics
            • Borna disease virus / isolation & purification
            • Cloning, Molecular
            • Consensus Sequence
            • DNA, Viral
            • Genetic Variation
            • Horse Diseases / virology
            • Horses
            • Humans
            • Leukocytes, Mononuclear / virology
            • Mental Disorders / virology
            • Molecular Sequence Data
            • Open Reading Frames
            • Viral Proteins / genetics

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