Sero-diagnosis of surra exploiting recombinant VSG antigen based ELISA for surveillance.
Abstract: Trypanosoma evansi, a haemoflagellate, causes "surra" an important chronic wasting disease of a wide range of wild and domestic herbivorous and carnivorous animals including cattle, buffaloes, camels, horses, etc. The untreated recovered animal can act as a carrier without exhibiting the disease symptoms and can be a source of infection to healthy animals. The diagnosis and subsequent treatment of the carrier animals is helpful to curb the disease. As the parasitaemia in carrier animals is very scanty, the conventional blood smear examination, which is widely practiced in the field, cannot detect such condition. For this purpose improved diagnostics are very much useful for mass sero-screening test such as ELISA. In the present study, the VSG of T. evansi was expressed in prokaryotic system (E. coli) and thereafter its immunoreactivity has been evaluated in immuno blot and enzyme immuno assay. The expressed protein showed 95.6% sensitivity, 98.0% specificity and 0.93 Cohen's kappa value, when compared with standard antigens. The developed antigen has also been validated with field serum samples from bovine, camel and horse collected from different states of India. The data showed that the developed recombinant antigen can be a diagnostic tool to detect carrier animals as well as control of the disease.
Copyright © 2014 Elsevier B.V. All rights reserved.
Publication Date: 2014-09-06 PubMed ID: 25269987DOI: 10.1016/j.vetpar.2014.08.012Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Animal Health
- Antibodies
- Antigen
- Biotechnology
- Diagnostic Technique
- Disease control
- Disease Diagnosis
- Disease Surveillance
- Domestic Animals
- Equine Diseases
- Equine Health
- Equine Research
- Molecular biology
- Recurrent Airway Obstruction
- Serodiagnosis
- Serological Surveys
- Seroprevalence
- Veterinary Research
- Veterinary Science
Summary
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The research study presents the development and testing of an ELISA-based diagnostic tool using a recombinantly-expressed antigen for the detection of Trypanosoma evansi, the cause of surra, a disease affecting a wide range of animal species.
Objective of Research
- The main objective of this study was to develop an improved diagnostic tool for the early and reliable detection of Trypanosoma evansi, a parasite that causes surra, an important chronic wasting disease in many animals. This was done by exploiting the recombinant variable surface glycoprotein (VSG) antigen in an Enzyme-Linked Immunosorbent Assay (ELISA) testing method to enhance sensitivity, specificity and detection rate of the infection, particularly in carrier animals with low parasitaemia.
Methodology
- The VSG of Trypanosoma evansi was expressed in a prokaryotic system (E. coli) to create the VSG antigen test. Its immunoreactivity was assessed using an immuno blot and enzyme immuno assay.
- The developed test was compared with standard antigens to evaluate its sensitivity, specificity, and Cohen’s kappa value (a measure of reliability).
- Validation of the developed antigen was performed using field serum samples collected from cattle, camels, and horses in different Indian states.
Results
- Findings from the study revealed that the developed antigen showed a high sensitivity of 95.6% and specificity of 98.0%, and a Cohen’s kappa value of 0.93 when compared with standard antigens. This suggests a high reliability of the test.
- Upon validation with field samples, the recombinant antigen proved to be a useful diagnostic tool for identifying carrier animals, hence can aid in effective disease control by enabling early treatment of these carriers before spreading the infection to healthy animals.
Implication
- The diagnostic tool developed in this research can help in the timely and accurate diagnosis of Trypanosoma evansi infection, especially in carriers with low parasitaemia levels that cannot be detected using conventional blood smear examination. This can be a significant contribution to the control and prevention of surra, an important chronic wasting disease in animals.
Cite This Article
APA
Sengupta PP, Rudramurthy GR, Ligi M, Roy M, Balamurugan V, Krishnamoorthy P, Nagalingam M, Singh L, Rahman H.
(2014).
Sero-diagnosis of surra exploiting recombinant VSG antigen based ELISA for surveillance.
Vet Parasitol, 205(3-4), 490-498.
https://doi.org/10.1016/j.vetpar.2014.08.012 Publication
Researcher Affiliations
- National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru 560024, Karnataka, India. Electronic address: pinakiprasad_s@rediffmail.com.
- National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru 560024, Karnataka, India.
- National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru 560024, Karnataka, India.
- National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru 560024, Karnataka, India.
- National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru 560024, Karnataka, India.
- National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru 560024, Karnataka, India.
- National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru 560024, Karnataka, India.
- Collaborating Unit, AICRP on ADMAS, State Disease Diagnostic Centre, Old B.P. Lab. Campus, Gopinath Marg, New Colony, Jaipur 302001, Rajasthan, India.
- National Institute of Veterinary Epidemiology and Disease Informatics, Hebbal, Bengaluru 560024, Karnataka, India.
MeSH Terms
- Animals
- Antibodies, Protozoan / blood
- Antigens, Protozoan / genetics
- Antigens, Protozoan / immunology
- Buffaloes
- Camelus / parasitology
- Cattle
- DNA, Protozoan / genetics
- Enzyme-Linked Immunosorbent Assay / veterinary
- Escherichia coli / genetics
- Escherichia coli / metabolism
- Gene Expression
- Horse Diseases / epidemiology
- Horse Diseases / parasitology
- Horses
- India / epidemiology
- Membrane Glycoproteins / genetics
- Membrane Glycoproteins / immunology
- Protozoan Proteins / genetics
- Protozoan Proteins / immunology
- Recombinant Proteins
- Sensitivity and Specificity
- Trypanosoma / genetics
- Trypanosoma / immunology
- Trypanosoma / isolation & purification
- Trypanosomiasis / epidemiology
- Trypanosomiasis / parasitology
- Trypanosomiasis / veterinary
- Trypanosomiasis, Bovine / epidemiology
- Trypanosomiasis, Bovine / parasitology
Citations
This article has been cited 1 times.- Chandu AGS, Sengupta PP, Jacob SS, Suresh KP, Borthakur SK, Patra G, Roy P. Seroprevalence of Trypanosoma evansi in cattle and analysis of associated climatic risk factors in Mizoram, India.. J Parasit Dis 2021 Mar;45(1):244-251.
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