Serologic response and lesions in goats experimentally infected with Corynebacterium pseudotuberculosis of caprine and equine origins.
Abstract: Fifteen goat kids were experimentally inoculated with Corynebacterium pseudotuberculosis. Five were given a strain of caprine origin (nitrate-negative biotype) intradermally, 5 were given a strain of equine origin (nitrate-positive biotype) intradermally, and 5 were inoculated intranasally with the caprine-origin strain. Animals were monitored for 127 days. The goats given the inocula intradermally developed abscesses; those given caprine-origin strain had multiple lesions both peripherally and in visceral locations (primarily endothoracic abscesses), whereas those given the equine-origin strain had abscesses only at injection sites and draining nodes. The difference in extent of lesions could be due to biotypic bacterial differences or to the individual strains used. Intranasally inoculated goats did not develop abscesses and were essentially no different from controls. The cranial part of the respiratory tract may not be an important portal of entry for C pseudotuberculosis. Serum samples obtained monthly from all animals were subjected to the synergistic hemolysis-inhibition test, which measures antibodies to the exotoxin of C pseudotuberculosis. Animals with abscesses developed titers within 1 month of inoculation. Animals without abscesses remained seronegative. The synergistic hemolysis-inhibition test may be a reliable diagnostic assay for caseous lymphadenitis in goats.
Publication Date: 1985-11-01 PubMed ID: 4073643
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- Comparative Study
- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research examined the effects of an infectious bacterium, Corynebacterium pseudotuberculosis, in goats. The bacterium was found to cause abscesses (swollen areas filled with pus) in goats, with different strains of the bacterium causing different types of abscesses.
Methodology
- Fifteen goat kids were chosen for the study and injected with C. pseudotuberculosis. The goats were divided into three groups of five.
- The first group was inoculated with a strain of the bacterium that originates from goats (caprine origin) through the skin (intradermally).
- The second group received a strain from horses (equine origin), also intradermally.
- The third group was given the caprine-origin strain through the nose (intranasally).
- The animals were monitored for a period of 127 days and their physical responses were recorded and analyzed.
Findings
- The goats that were inoculated intradermally developed abscesses. The goats inoculated with the caprine-origin strain developed multiple lesions in various parts of their bodies, primarily in the chest area (endothoracic abscesses). The ones that received the equine-origin strain developed abscesses only at the injection sites and in lymph nodes near the sites.
- The goats inoculated intranasally did not develop abscesses and showed no significant difference from control goats. The study suggests that the respiratory tract may not be a significant entry point for C. pseudotuberculosis.
- The researchers also used the synergistic hemolysis-inhibition test to measure antibodies to the exotoxin of C. pseudotuberculosis in serum samples taken from the goats. Those with abscesses developed titers, or concentrations of antibodies, within a month of inoculation. Goats without abscesses did not show these titers, remaining seronegative.
Implications
- The difference in the extent of lesions suggests that the biotype of the bacterium or the specific strains used could affect the pathogenicity of C. pseudotuberculosis.
- The synergistic hemolysis-inhibition test could potentially be used as a reliable diagnostic test for caseous lymphadenitis in goats, a disease caused by C. pseudotuberculosis.
Cite This Article
APA
Brown CC, Olander HJ, Biberstein EL, Moreno D.
(1985).
Serologic response and lesions in goats experimentally infected with Corynebacterium pseudotuberculosis of caprine and equine origins.
Am J Vet Res, 46(11), 2322-2326.
Publication
Researcher Affiliations
MeSH Terms
- Animals
- Corynebacterium Infections / immunology
- Corynebacterium Infections / microbiology
- Corynebacterium Infections / veterinary
- Exotoxins / immunology
- Goats / immunology
- Goats / microbiology
- Hemolysis
- Horses / microbiology
- Male
Citations
This article has been cited 4 times.- Burmayan A, Brundage CM. Caseous lymphadenitis outbreak in a small ruminant herd.. Open Vet J 2021 Oct-Dec;11(4):530-534.
- Brown CC, Olander HJ, Alves SF. Synergistic hemolysis-inhibition titers associated with caseous lymphadenitis in a slaughterhouse survey of goats and sheep in Northeastern Brazil.. Can J Vet Res 1987 Jan;51(1):46-9.
- Holstad G, Teige J Jr. Corynebacterium pseudotuberculosis infection in goats. VII. Clinical, pathological, serological and hematological changes after subcutaneous inoculation of the organism.. Acta Vet Scand 1988;29(3-4):287-94.
- Menzies PI, Muckle CA. The use of a microagglutination assay for the detection of antibodies to Corynebacterium pseudotuberculosis in naturally infected sheep and goat flocks.. Can J Vet Res 1989 Jul;53(3):313-8.
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