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Home / Equine Research Bank / Arch Virol / Serological relationships between rotaviruses from different...
Archives of virology1977; 53(4); 287-294; doi: 10.1007/BF01315627

Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization.

Abstract: Human, piglet, mouse, foal, lamb, calf and rabbit rotaviruses all infected, but could not readily be subcultured in LLC MK2 cells. Cells infected with mouse and calf rotaviruses reacted by indirect immunofluorescence (FA) with convalescent serum from children, piglets, mice, foals, lambs, calves or rabbits, taken after rotavirus infection. Human, calf, piglet, mouse and foal rotaviruses reacted with human, calf, mouse, foal and lamb convalescent serum by complement fixation (CF). It was not possible to distinguish between different rotaviruses by CF or FA. Neutralization tests, however, detected species-specific rotavirus antigens. Any virus was neutralized by a much higher dilution of homologous species convalescent serum than by any heterologous serum. With the exception of the mouse virus there was very little cross reaction. However, in sera with a very high neutralizing titre for the homologous virus the titre was proportionately raised against heterologous virus. It is, therefore, now possible to type to species an unknown rotavirus by a neutralization test in LLC MK2 cells using convalescent serum from each species.
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Publication Date: 1977-01-01 PubMed ID: 68765DOI: 10.1007/BF01315627Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article studies the serological connections within rotaviruses from various species using the complement fixation and neutralization tests.

Research Focus

  • The study primarily focuses on understanding the relationships between rotaviruses derived from distinct species, including humans, piglets, mice, foals, lambs, calves, and rabbits.
  • These viruses were examined in terms of their effect on LLC MK2 cells and the subsequent responses of the cells following rotavirus infection.
  • The research also sought to ascertain whether differentiation between various rotaviruses was possible using complement fixation (CF) or indirect immunofluorescence (FA) methods.

Experimental Findings and Observations

  • All the viruses from different species mentioned were found to infect the LLC MK2 cells but could not be subcultured easily.
  • The cells infected with rotaviruses from mice and calves reacted to the indirect immunofluorescence with the convalescent serum (serum collected post-infection) of all the species tested.
  • Additionally, the human, calf, piglet, mouse, and foal rotavirus reacted with corresponding convalescent serum by the complement fixation approach.
  • However, differentiation between varying rotaviruses using CF or FA proved challenging.

Neutralization Test Results

  • Unlike CF or FA methods, neutralization tests helped in detecting species-specific rotavirus antigens.
  • The analysis suggested that a virus was neutralized more effectively by a greater dilution of homologous species convalescent serum than any heterologous serum.
  • There was minimal cross-reaction observed for these neutralizations, barring the mouse virus.
  • In high neutralizing titre cases for a homologous (similar) virus, the titre’s proportion also increased against a heterologous (different) virus.

Implications and Conclusions

  • The findings make it feasible to classify an unidentifiable rotavirus to a species via a neutralization test in LLC MK2 cells, using convalescent serum obtained from each species.
  • The limited cross-reaction observed between the viruses, along with the marked differences in neutralizing capabilities of homologous and heterologous serum, underscores the immense strength and utility of the neutralization test for rotavirus detection and classification.

Cite This Article

APA
Thouless ME, Bryden AS, Flewett TH, Woode GN, Bridger JC, Snodgrass DR, Herring JA. (1977). Serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. Arch Virol, 53(4), 287-294. https://doi.org/10.1007/BF01315627

Publication

Archives of virology
ISSN: 0304-8608
NlmUniqueID: 7506870
Country: Austria
Language: English
Volume: 53
Issue: 4
Pages: 287-294

Researcher Affiliations

Thouless, M E
    Bryden, A S
      Flewett, T H
        Woode, G N
          Bridger, J C
            Snodgrass, D R
              Herring, J A

                MeSH Terms

                • Animals
                • Antigens, Viral / analysis
                • Cattle
                • Cell Line
                • Complement Fixation Tests
                • Epitopes
                • Feces / microbiology
                • Gastroenteritis / microbiology
                • Horses
                • Humans
                • Mice
                • Neutralization Tests
                • Rabbits
                • Sheep
                • Swine
                • Virus Cultivation
                • Viruses, Unclassified / growth & development
                • Viruses, Unclassified / immunology

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