Seroprevalence of Anaplasma phagocytophilum in well maintained horses from northern Tunisia.
Abstract: The aim of the present study was to determine the seroprevalence of Anaplasma phagocytophilum in 343 well maintained horses belonging to 11 horse stables located in northern Tunisia with indirect immunofluorescence test. Overall, 16.3% (56/343) of tested animals were positive. Anti-A. phagocytophilum antibodies were present in horses located in all studied governorates except the governorate of Ben Arous. Nine horse stables out of 11 contained positive animals, the seroprevalence of each one varied from nought to 50%. Seroprevalence varied according to gender, breed and type of activity, contrary to age. Seroprevalence was higher in females than males (21.4 and 9.5% respectively). Similarly, seropositive animals belonging to the English thoroughbred breed was significantly higher (30.2%) than other breeds. According to the utilization of horses, the highest positivity rate (21.1%) was observed in brood mares. Our results demonstrate that A. phagocytophilum infection is widespread in well maintained horses from northern Tunisia, so equine granulocytic anaplasmosis should be suspected by veterinarians in front of suggestive clinical signs like fever, ataxia and reluctance to move.
Publication Date: 2014-11-11 PubMed ID: 25382469
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study aims to understand the prevalence of Anaplasma phagocytophilum, a disease affecting horses, in the northern region of Tunisia. The findings indicate that 16.3% of the horses tested were positive, with variances observed based on gender, breed, and type of horse activity.
Objective of the Research
- The research aimed to detect the prevalence of Anaplasma Phagocytophilum, a bacterial infection, in well-maintained horses from northern Tunisia. The study used an indirect immunofluorescence test to check 343 horses from 11 stables in the region.
Key Findings
- Out of the horses tested, 16.3% were found to be positive for the disease. This implies that the infection is quite widespread among the horsing community in the region.
- All the districts surveyed had horses that tested positive for the disease except for the governorate of Ben Arous. Likewise, all the tested stables except two had at least one horse that tested positive.
- The study found a wide range of prevalence rates among the stables tested, from as low as 0% to as high as 50%.
Variations in Seroprevalence
- Differences in infection rates were noticed between breed, gender, and type of activity the horse was involved in, but not the age of the horse.
- Female horses were more likely to test positive than males, with rates of 21.4% and 9.5% respectively.
- In terms of breed, English thoroughbred horses showed a significantly higher rate of positivity (30.2%) compared to the others.
- When the horses’ activities were considered, brood mares had an infection rate of 21.1%, the highest among all categories.
Implications of the Findings
- The results support the fact that Anaplasma Phagocytophilum infection is quite common in the horse population of northern Tunisia.
- The researchers recommend that veterinarians should suspect such an infection in horses showing symptoms like fever, ataxia (loss of full control over body movements), and reluctance to move.
- The study highlights the need for further investigations to address the disease and design suitable measures to prevent its spread.
Cite This Article
APA
Ben Said M, Belkahia H, Héni MM, Bouattour A, Ghorbel A, Gharbi M, Zouari A, Darghouth MA, Messadi L.
(2014).
Seroprevalence of Anaplasma phagocytophilum in well maintained horses from northern Tunisia.
Trop Biomed, 31(3), 432-440.
Publication
Researcher Affiliations
- Laboratoire de Microbiologie, Ecole Nationale de Médecine Vétérinaire, Université de la Manouba, 2020 Sidi Thabet, Tunisia.
- Laboratoire de Microbiologie, Ecole Nationale de Médecine Vétérinaire, Université de la Manouba, 2020 Sidi Thabet, Tunisia.
- Laboratoire de Microbiologie, Ecole Nationale de Médecine Vétérinaire, Université de la Manouba, 2020 Sidi Thabet, Tunisia.
- Laboratoire d'Epidémiologie et de Microbiologie Vétérinaire, Service d'Entomologie Médicale, Institut Pasteur de Tunis, 1002 Tunis, Tunisia.
- Laboratoire de Pathologie Médicale des Carnivores et des Équidés, Ecole Nationale de Médecine Vétérinaire, Université de la Manouba, 2020 Sidi Thabet, Tunisia.
- Laboratoire de Parasitologie, Ecole Nationale de Médecine Vétérinaire, Université de la Manouba, 2020 Sidi Thabet, Tunisia.
- Direction de la Santé, Ministère de l'Intérieur, Tunis, Tunisia.
- Laboratoire de Parasitologie, Ecole Nationale de Médecine Vétérinaire, Université de la Manouba, 2020 Sidi Thabet, Tunisia.
- Laboratoire de Microbiologie, Ecole Nationale de Médecine Vétérinaire, Université de la Manouba, 2020 Sidi Thabet, Tunisia.
MeSH Terms
- Anaplasma phagocytophilum / immunology
- Animals
- Antibodies, Bacterial / blood
- Ehrlichiosis / epidemiology
- Ehrlichiosis / microbiology
- Ehrlichiosis / veterinary
- Female
- Fluorescent Antibody Technique, Indirect
- Horse Diseases / epidemiology
- Horse Diseases / microbiology
- Horses
- Male
- Seroepidemiologic Studies
- Tunisia / epidemiology
Citations
This article has been cited 3 times.- Abdelbaset AE, Kwak ML, Nonaka N, Nakao R. Human-biting ticks and zoonotic tick-borne pathogens in North Africa: diversity, distribution, and trans-Mediterranean public health challenges. One Health 2023 Jun;16:100547.
- Seo MG, Ouh IO, Choi E, Kwon OD, Kwak D. Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Korea. Korean J Parasitol 2018 Dec;56(6):559-565.
- Ben Said M, Belkahia H, Alberti A, Zobba R, Bousrih M, Yahiaoui M, Daaloul-Jedidi M, Mamlouk A, Gharbi M, Messadi L. Molecular Survey of Anaplasma Species in Small Ruminants Reveals the Presence of Novel Strains Closely Related to A. phagocytophilum in Tunisia. Vector Borne Zoonotic Dis 2015 Oct;15(10):580-90.
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