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Home / Equine Research Bank / Parasite / Seroprevalence of Theileria equi and Babesia caballi in hors...
Parasite (Paris, France)2017; 24; 14; doi: 10.1051/parasite/2017015

Seroprevalence of Theileria equi and Babesia caballi in horses in Spain.

Abstract: Equine piroplasmoses are enzootic parasitic diseases distributed worldwide with high incidence in tropical and subtropical regions. In Spain, there is insufficient epidemiological data about equine piroplasmoses. The main aim of the present study was therefore to estimate the prevalence of Theileria equi and Babesia caballi in five regions and obtain information about the risk factors. This study was conducted in the central and south-western regions of Spain, using indirect fluorescence antibody testing (IFAT) in 3,100 sera samples from apparently healthy horses of different ages, breeds, coat colours, genders and geographical locations. The overall seroprevalence was 52%, consisting of 44% seropositive for T. equi and 21% for B. caballi. There was a significant association between age (p < 0.0001), breed (p < 0.004), geographical location (p < 0.0001) and the seroprevalence, but neither the coat colour nor the gender was significantly associated with prevalence. In addition, it was proved that most of the geographic areas showed a moderate to high prevalence. The statistical κ value was used to compare the results obtained by the IFAT and the competitive enzyme-linked immunosorbent assay (cELISA) utilised to test some samples (n = 108) and showed a higher concordance for T. equi (κ = 0.68) than for B. caballi (κ = 0.22). Consequently, this revealed the importance of developing an appropriate technique to detect each haemoparasite. Les piroplasmoses équines sont des parasitoses enzootiques cosmopolites qui existent surtout dans les régions tropicales et subtropicales. En Espagne, peu de données épidémiologiques sont disponibles sur les piroplasmoses équines. Ainsi, le principal objectif de la présente étude a été d’estimer la séroprévalence de l’infection par Theileria equi et Babesia caballi dans cinq régions et obtenir des informations sur les facteurs de risque. Cette étude a été menée dans les régions du centre et du sud-ouest de l’Espagne en utilisant le test d’immunofluorescence indirecte (IFI) sur un échantillon de 3100 sérums de chevaux apparemment sains de différents âges, races, robes, sexe et origines géographiques. La séroprévalence globale était de 52 %, y compris 44 % séropositifs pour T. equi et 21 % pour B. caballi. Il y avait une association significative entre l’âge (p < 0.0001), la race (p < 0.004), la localisation géographique (p < 0.0001) et la séroprévalence. Cependant, ni la couleur du pelage ni le sexe n’avaient de corrélation significative avec la séroprévalence. En outre, la plupart des régions étaient dans un état de prévalence modérée à élevée. Le coefficient de concordance κ a été utilisé pour comparer les résultats obtenus par IFI et cELISA pour tester 108 échantillons, et a montré une concordance plus élevée pour T. equi (κ = 0.68) que pour B. caballi (κ = 0.22). Cela démontre l’importance de développer une technique appropriée pour détecter chaque hémoparasite.
© M.G. Montes Cortés et al., published by EDP Sciences, 2017.
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Publication Date: 2017-05-12 PubMed ID: 28497743PubMed Central: PMC5432961DOI: 10.1051/parasite/2017015Google Scholar: Lookup
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Summary

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This study investigates the prevalence of parasites Theileria equi and Babesia caballi in horses across different Spanish regions, finding a 52% prevalence overall. Factors such as age, breed, and geographical location significantly affected prevalence rates.

Research Background

  • Equine piroplasmoses are parasitic diseases that occur worldwide, particularly in tropical and subtropical regions. The study focuses on Theileria equi and Babesia caballi, two parasites causing these diseases. In Spain, there has been a lack of data on them.

Research Aims and Methods

  • The study’s main goal was to determine the prevalence of T. equi and B. caballi in five of Spain’s regions and identify risk factors.
  • It analyzed 3,100 sera samples from seemingly healthy horses of various ages, breeds, coats, genders, and geographical areas using an indirect fluorescence antibody testing (IFAT). Some samples were also evaluated using a competitive enzyme-linked immunosorbent assay (cELISA).

Findings

  • The overall seroprevalence was 52%, with 44% of the samples testing positive for T. equi and 21% for B. caballi.
  • There was a significant association between prevalence and age, breed, and geographical location. Neither the coat color nor the gender of the horses had a significant correlation with the prevalence of these parasites.
  • Most geographic areas showed moderate to high prevalence of these infections.
  • The study also compared the results derived from IFAT and cELISA techniques, finding a higher concordance for T. equi (κ = 0.68) than for B. caballi (κ = 0.22), highlighting the need for a suitable detection technique for each parasite.

Cite This Article

APA
Montes Cortés MG, Fernández-García JL, Habela Martínez-Estéllez MÁ. (2017). Seroprevalence of Theileria equi and Babesia caballi in horses in Spain. Parasite, 24, 14. https://doi.org/10.1051/parasite/2017015

Publication

Parasite (Paris, France)
ISSN: 1776-1042
NlmUniqueID: 9437094
Country: France
Language: English
Volume: 24
Pages: 14

Researcher Affiliations

Montes Cortés, Maria Guadalupe
  • Parasitology and Parasitic Diseases, Animal Health Department, Veterinary Faculty, University of Extremadura, 10071 Cáceres, Spain.
Fernández-García, José Luis
  • Genetics and Animal Breeding, Veterinary Faculty, University of Extremadura, 10071 Cáceres, Spain.
Habela Martínez-Estéllez, Miguel Ángel
  • Parasitology and Parasitic Diseases, Animal Health Department, Veterinary Faculty, University of Extremadura, 10071 Cáceres, Spain.

MeSH Terms

  • Age Factors
  • Animals
  • Antibodies, Protozoan / blood
  • Babesia / immunology
  • Babesiosis / epidemiology
  • Babesiosis / parasitology
  • Breeding
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Female
  • Fluorescent Antibody Technique, Indirect / veterinary
  • Horse Diseases / epidemiology
  • Horse Diseases / parasitology
  • Horses
  • Male
  • Risk Factors
  • Seroepidemiologic Studies
  • Spain / epidemiology
  • Theileria / immunology
  • Theileriasis / epidemiology
  • Theileriasis / parasitology

References

This article includes 105 references
  1. Abedi V, Razmi G, Seifi H, Naghibi A. Molecular and serological detection of Theileria equi and Babesia caballi infection in horses and ixodid ticks in Iran.. Ticks Tick Borne Dis 2014 Apr;5(3):239-44.
    pubmed: 24556274doi: 10.1016/j.ttbdis.2013.11.008google scholar: lookup
  2. Abutarbush SM, Alqawasmeh DM, Mukbel RM, Al-Majali AM. Equine babesiosis: seroprevalence, risk factors and comparison of different diagnostic methods in Jordan.. Transbound Emerg Dis 2012 Feb;59(1):72-8.
    pubmed: 21771287doi: 10.1111/j.1865-1682.2011.01244.xgoogle scholar: lookup
  3. Acici M, Umur S, Guvenc T, Arslan HH, Kurt M. Seroprevalence of equine babesiosis in the Black Sea region of Turkey.. Parasitol Int 2008 Jun;57(2):198-200.
    pubmed: 18234550doi: 10.1016/j.parint.2007.12.009google scholar: lookup
  4. Aharonson-Raz K, Rapoport A, Hawari IM, Lensky IM, Berlin D, Zivotofsky D, Klement E, Steinman A. Novel description of force of infection and risk factors associated with Theileria equi in horses in Israel and in The Palestinian Authority.. Ticks Tick Borne Dis 2014 Jun;5(4):366-72.
    pubmed: 24703834doi: 10.1016/j.ttbdis.2014.01.002google scholar: lookup
  5. Akkan HA, Karaca M, Tutuncu M, Deger S, Keles I, Agaoglu Z. Serologic and microscopic studies on babesiosis in horses in the eastern border of Turkey.. Journal of Equine Veterinary Sciences 5(23), 181–183.
  6. Alanazi AD, Alyousif MS, Hassieb MM. Seroprevalence study on Theileria equi and Babesia caballi antibodies in horses from central province of Saudi Arabia.. J Parasitol 2012 Oct;98(5):1015-7.
    pubmed: 22489955doi: 10.1645/ge-2997.1google scholar: lookup
  7. Alhassan A, Pumidonming W, Okamura M, Hirata H, Battsetseg B, Fujisaki K, Yokoyama N, Igarashi I. Development of a single-round and multiplex PCR method for the simultaneous detection of Babesia caballi and Babesia equi in horse blood.. Vet Parasitol 2005 Apr 20;129(1-2):43-9.
    pubmed: 15817201doi: 10.1016/j.vetpar.2004.12.018google scholar: lookup
  8. Asgarali Z, Coombs DK, Mohammed F, Campbell MD, Caesar E. A serological study of Babesia caballi and Theileria equi in Thoroughbreds in Trinidad.. Vet Parasitol 2007 Mar 15;144(1-2):167-71.
    pubmed: 17118557doi: 10.1016/j.vetpar.2006.09.015google scholar: lookup
  9. Ayala-Valdovinos MA, Lemus-Flores C, Galindo-García J, Bañuelos-Pineda J, Rodríguez-Carpena JG, Sánchez-Chiprés D, Duifhuis-Rivera T. Diagnosis and prevalence of Theileria equi horses in western Mexico by nested PCR.. Parasitol Int 2017 Feb;66(1):821-824.
    pubmed: 27671686doi: 10.1016/j.parint.2016.09.011google scholar: lookup
  10. Bahrami S, Ghadrdan AR, Mirabdollahi SM, Fayed MR. Diagnosis of subclinical equine theileriosis in center of Iran using parasitological and molecular methods.. Trop Biomed 2014 Mar;31(1):110-7.
    pubmed: 24862050
  11. Baptista C, Lopes MS, Tavares AC, Rojer H, Kappmeyer L, Mendonça D, da Câmara Machado A. Diagnosis of Theileria equi infections in horses in the Azores using cELISA and nested PCR.. Ticks Tick Borne Dis 2013 Apr;4(3):242-5.
    pubmed: 23337492doi: 10.1016/j.ttbdis.2012.11.008google scholar: lookup
  12. Bartolomé Del Pino LE, Nardini R, Veneziano V, Iacoponi F, Cersini A, Autorino GL, Buono F, Scicluna M. Babesia caballi and Theileria equi infections in horses in Central-Southern Italy: Sero-molecular survey and associated risk factors.. Ticks Tick Borne Dis 2016 Apr;7(3):462-9.
    pubmed: 26847198doi: 10.1016/j.ttbdis.2016.01.011google scholar: lookup
  13. Bashiruddin JB, Cammà C, Rebêlo E. Molecular detection of Babesia equi and Babesia caballi in horse blood by PCR amplification of part of the 16S rRNA gene.. Vet Parasitol 1999 Jul;84(1-2):75-83.
    pubmed: 10435792doi: 10.1016/s0304-4017(99)00049-7google scholar: lookup
  14. Bhoora R, Franssen L, Oosthuizen MC, Guthrie AJ, Zweygarth E, Penzhorn BL, Jongejan F, Collins NE. Sequence heterogeneity in the 18S rRNA gene within Theileria equi and Babesia caballi from horses in South Africa.. Vet Parasitol 2009 Feb 5;159(2):112-20.
    pubmed: 19019541doi: 10.1016/j.vetpar.2008.10.004google scholar: lookup
  15. Bhoora R, Quan M, Zweygarth E, Guthrie AJ, Prinsloo SA, Collins NE. Sequence heterogeneity in the gene encoding the rhoptry-associated protein-1 (RAP-1) of Babesia caballi isolates from South Africa.. Vet Parasitol 2010 May 11;169(3-4):279-88.
    pubmed: 20138703doi: 10.1016/j.vetpar.2010.01.009google scholar: lookup
  16. Boldbaatar D, Xuan X, Battsetseg B, Igarashi I, Battur B, Batsukh Z, Bayambaa B, Fujisaki K. Epidemiological study of equine piroplasmosis in Mongolia.. Vet Parasitol 2005 Jan 4;127(1):29-32.
    pubmed: 15619372doi: 10.1016/j.vetpar.2004.08.021google scholar: lookup
  17. Botteon PTL, Massard CL, Botteon RCCM, Loss ZG, Linhares GFC. Seroprevalence of Babesia equi in three breeding systems of equines. Rio de Janeiro-Brazil.. Parasitología Latinoamericana 57(3–4), 141–145.
  18. Brüning A. Equine piroplasmosis an update on diagnosis, treatment and prevention.. Br Vet J 1996 Mar;152(2):139-51.
    pubmed: 8680838doi: 10.1016/s0007-1935(96)80070-4google scholar: lookup
  19. Butler CM, Sloet van Oldruitenborgh-Oosterbaan MM, Stout TA, van der Kolk JH, Wollenberg Lv, Nielen M, Jongejan F, Werners AH, Houwers DJ. Prevalence of the causative agents of equine piroplasmosis in the South West of The Netherlands and the identification of two autochthonous clinical Theileria equi infections.. Vet J 2012 Aug;193(2):381-5.
    pubmed: 22266019doi: 10.1016/j.tvjl.2011.12.014google scholar: lookup
  20. Cacciò S, Cammà C, Onuma M, Severini C. The beta-tubulin gene of Babesia and Theileria parasites is an informative marker for species discrimination.. Int J Parasitol 2000 Oct;30(11):1181-5.
    pubmed: 11027785doi: 10.1016/s0020-7519(00)00105-3google scholar: lookup
  21. Camacho AT, Guitian FJ, Pallas E, Gestal JJ, Olmeda AS, Habela MA, Telford SR 3rd, Spielman A. Theileria (Babesia) equi and Babesia caballi infections in horses in Galicia, Spain.. Trop Anim Health Prod 2005 May;37(4):293-302.
    pubmed: 15934637doi: 10.1007/s11250-005-5691-zgoogle scholar: lookup
  22. Camino E, Carvajal KA, Lozano FJ, Viñolo C, Alende T, Domínguez L, Cruz F. Estudio de seroprevalencia de piroplamosis equina en España en muestras previas a la exportación.. XXI Simposio Anual Avedila, Murcia .
  23. Cantú-Martínez MA, Segura-Correa JC, Silva-Páez ML, Avalos-Ramírez R, Wagner GG. Prevalence of antibodies to Theileria equi and Babesia caballi in horses from northeastern Mexico.. J Parasitol 2012 Aug;98(4):869-70.
    pubmed: 22339765doi: 10.1645/ge-3064.1google scholar: lookup
  24. Cohen J. A coefficient of agreement for nominal scales.. Educational and Psychological Measurement 20, 37–46.
  25. Criado-Fornelio A, Gónzalez-del-Río MA, Buling-Saraña A, Barba-Carretero JC. The "expanding universe" of piroplasms.. Vet Parasitol 2004 Feb 6;119(4):337-45.
    pubmed: 15154598doi: 10.1016/j.vetpar.2003.11.015google scholar: lookup
  26. Davitkov D, Vucicevic M, Stevanovic J, Krstic V, Slijepcevic D, Glavinic U, Stanimirovic Z. Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan.. Acta Parasitol 2016 Mar;61(2):337-42.
    pubmed: 27078657doi: 10.1515/ap-2016-0044google scholar: lookup
  27. De Campos CHC, Prado RFS, Guimãraes A, da Silva AT, Baldani CD, Cordeiro MD, Pires MS, Peixoto MP, dos Santos HA, Machado RZ, Fonseca AH, Massard CL. Aspectos epidemiológicos e soroprevalência de Theileria equi em equinos de uso military no munícipio de Resende, estado do Rio de Janeiro, Brasil.. Revista Brasileira de Medicina Veterinária 35(S2), 106–112.
  28. De Waal DT, Van Heerden J. Equine Piroplasmosis, in Infectious Diseases of Livestock. Oxford University Press p. 295–304.
  29. Dos Santos TM, Roier EC, Santos HA, Pires MS, Vilela JA, Moraes LM, Almeida FQ, Baldani CD, Machado RZ, Massard CL. Factors associated to Theileria equi in equids of two microregions from Rio de Janeiro, Brazil.. Rev Bras Parasitol Vet 2011 Jul-Sep;20(3):235-41.
    pubmed: 21961755doi: 10.1590/s1984-29612011000300011google scholar: lookup
  30. Farkas R, Tánczos B, Gyurkovszky M, Földvári G, Solymosi N, Edelhofer R, Hornok S. Serological and molecular detection of Theileria equi infection in horses in Hungary.. Vet Parasitol 2013 Feb 18;192(1-3):143-8.
    pubmed: 23099089doi: 10.1016/j.vetpar.2012.09.035google scholar: lookup
  31. Friedhoff KT, Tenter AM, Müller I. Haemoparasites of equines: impact on international trade of horses.. Rev Sci Tech 1990 Dec;9(4):1187-94.
    pubmed: 2132711
  32. Friedhoff KT, Soulé C. An account on equine babesioses.. Rev Sci Tech 1996 Sep;15(3):1191-201.
    pubmed: 9025154doi: 10.20506/rst.15.3.972google scholar: lookup
  33. Fritz D. A PCR study of piroplasms in 166 dogs and 111 horses in France (March 2006 to March 2008).. Parasitol Res 2010 May;106(6):1339-42.
    pubmed: 20221639doi: 10.1007/s00436-010-1804-3google scholar: lookup
  34. Gallusová M, Qablan MA, D'Amico G, Oborník M, Petrželková KJ, Mihalca AD, Modrý D. Piroplasms in feral and domestic equines in rural areas of the Danube Delta, Romania, with survey of dogs as a possible reservoir.. Vet Parasitol 2014 Dec 15;206(3-4):287-92.
    pubmed: 25468026doi: 10.1016/j.vetpar.2014.10.018google scholar: lookup
  35. García-Bocanegra I, Arenas-Montes A, Hernández E, Adaszek L, Carbonero A, Almería S, Jaén-Téllez JA, Gutiérrez-Palomino P, Arenas A. Seroprevalence and risk factors associated with Babesia caballi and Theileria equi infection in equids.. Vet J 2013 Feb;195(2):172-8.
    pubmed: 22784418doi: 10.1016/j.tvjl.2012.06.012google scholar: lookup
  36. Golynski AA, Fernandes KR, Baldani CD, Golynski AL, Madeiro AS, Machado RZ, Botteon Pde T, Massard CL. [Seroepidemiological studies on Babesia equi in horses from the State of Rio Grande do Sul determined by indirect immunoflourecence test and Elisa].. Rev Bras Parasitol Vet 2008 Sep;17 Suppl 1:317-21.
    pubmed: 20059868
  37. Güçlü HZ, Karaer KZ. [Detection of Babesia caballi (Nuttall, 1910) and Theileria equi (Syn. Babesia equi , Laveran, 1901) by the polymerase chain reaction (PCR) in show and sport horses in the region of Ankara].. Turkiye Parazitol Derg 2007;31(2):89-93.
    pubmed: 17594644
  38. Guidi E, Pradier S, Lebert I, Leblond A. Piroplasmosis in an endemic area: analysis of the risk factors and their implications in the control of Theileriosis and Babesiosis in horses.. Parasitol Res 2015 Jan;114(1):71-83.
    pubmed: 25280516doi: 10.1007/s00436-014-4161-9google scholar: lookup
  39. Guven E, Avcioglu H, Deniz A, Balkaya İ, Abay U, Yavuz Ş, Akyüz M. Prevalence and molecular characterization of Theileria equi and Babesia caballi in jereed horses in Erzurum, Turkey.. Acta Parasitol 2017 Mar 1;62(1):207-213.
    pubmed: 28030350doi: 10.1515/ap-2017-0025google scholar: lookup
  40. Grandi G, Molinari G, Tittarelli M, Sassera D, Kramer LH. Prevalence of Theileria equi and Babesia caballi infection in horses from northern Italy.. Vector Borne Zoonotic Dis 2011 Jul;11(7):955-6.
    pubmed: 21254835doi: 10.1089/vbz.2010.0193google scholar: lookup
  41. Habela M, Reina D, Nieto D, Verdugo SG, Navarrete I. Epidemiología de la babesiosis equina en Extremadura: estudio preliminar.. Medicina Veterinaria 6, 31–39.
  42. Habela M, Grande A, Antón JM, Mora JA, Moreno F, Pérez-Martín JE. Aportaciones al conocimiento de la distribución y clínica de la babesiosis equina en España.. IV Congreso Ibérico de Parasitología, Santiago de Compostela .
  43. Habela MA, Gragera-Slikker A, Moreno A, Montes G, Sevilla R. Piroplasmosis equina: conocimiento y grado de concienciación de los productores de caballos Pura Raza Española.. Revista Equinus 11, 17–34.
  44. Heim A, Passos LM, Ribeiro MF, Costa-Júnior LM, Bastos CV, Cabral DD, Hirzmann J, Pfister K. Detection and molecular characterization of Babesia caballi and Theileria equi isolates from endemic areas of Brazil.. Parasitol Res 2007 Dec;102(1):63-8.
    pubmed: 17828553doi: 10.1007/s00436-007-0726-1google scholar: lookup
  45. Heuchert CM, de Giulli V Jr, de Athaide DF, Böse R, Friedhoff KT. Seroepidemiologic studies on Babesia equi and Babesia caballi infections in Brazil.. Vet Parasitol 1999 Aug 16;85(1):1-11.
    pubmed: 10447188doi: 10.1016/s0304-4017(99)00108-9google scholar: lookup
  46. Hussain MH, Saqib M, Raza F, Muhammad G, Asi MN, Mansoor MK, Saleem M, Jabbar A. Seroprevalence of Babesia caballi and Theileria equi in five draught equine populated metropolises of Punjab, Pakistan.. Vet Parasitol 2014 May 28;202(3-4):248-56.
    pubmed: 24582524doi: 10.1016/j.vetpar.2014.01.026google scholar: lookup
  47. Ikadai H, Nagai A, Xuan X, Igarashi I, Tsugihiko K, Tsuji N, Oyamada T, Suzuki N, Fujisaki K. Seroepidemiologic studies on Babesia caballi and Babesia equi infections in Japan.. J Vet Med Sci 2002 Apr;64(4):325-8.
    pubmed: 12014577doi: 10.1292/jvms.64.325google scholar: lookup
  48. Jaffer O, Abdishakur F, Hakimuddin F, Riya A, Wernery U, Schuster RK. A comparative study of serological tests and PCR for the diagnosis of equine piroplasmosis.. Parasitol Res 2010 Feb;106(3):709-13.
    pubmed: 19894063doi: 10.1007/s00436-009-1669-5google scholar: lookup
  49. Javed K, Ijaz M, Ali MM, Khan I, Mehmood K, Ali S. Prevalence and hematology of tick borne hemoparasitic diseases in Equines in and around Lahore, Pakistan.. Journal of Zoology 46(2), 401–408.
  50. Kamyingkird K, Yangtara S, Desquesnes M, Cao S, Adjou PK, Jittapalapong S, Nimsupan B, Terkawi MA, Masatani T, Nishikawa Y, Igarashi I, Xuan X. Seroprevalence of Babesia caballi and Theileria equi in horses and mules from Northern Thailand.. Journal of Protozoology Research 24, 11–17.
  51. Karatepe B, Karatepe M, Cakmak A, Karaer Z, Ergün G. Investigation of seroprevalence of Theileria equi and Babesia caballi in horses in Nigde province, Turkey.. Trop Anim Health Prod 2009 Jan;41(1):109-13.
    pubmed: 19052908doi: 10.1007/s11250-008-9164-zgoogle scholar: lookup
  52. Kerber CE, Ferreira F, Pereira MC. Control of equine piroplasmosis in Brazil.. Onderstepoort J Vet Res 1999 Jun;66(2):123-7.
    pubmed: 10486829
  53. Kerber CE, Labruna MB, Ferreira F, De Waal DT, Knowles DP, Gennari SM. Prevalence of equine Piroplasmosis and its association with tick infestation in the State of São Paulo, Brazil.. Rev Bras Parasitol Vet 2009 Oct-Dec;18(4):1-8.
    pubmed: 20040201doi: 10.4322/rbpv.01804001google scholar: lookup
  54. Kim CM, Blanco LB, Alhassan A, Iseki H, Yokoyama N, Xuan X, Igarashi I. Diagnostic real-time PCR assay for the quantitative detection of Theileria equi from equine blood samples.. Vet Parasitol 2008 Feb 14;151(2-4):158-63.
    pubmed: 18077095doi: 10.1016/j.vetpar.2007.10.023google scholar: lookup
  55. Kizilarslan F, Yildirim A, Duzlu O, Inci A, Onder Z, Ciloglu A. Molecular detection and characterization of Theileria equi and Babesia caballi in horses (Equus ferus caballus) in Turkey.. Journal of Equine Veterinary Science 35, 830–835.
  56. Kouam MK, Kantzoura V, Gajadhar AA, Theis JH, Papadopoulos E, Theodoropoulos G. Seroprevalence of equine piroplasms and host-related factors associated with infection in Greece.. Vet Parasitol 2010 May 11;169(3-4):273-8.
    pubmed: 20138434doi: 10.1016/j.vetpar.2010.01.011google scholar: lookup
  57. Kurt C, Yaman M. The investigation of the prevalence of Babesia equi and Babesia caballi in horses by microscopic and serologic (cELISA) methods in Adana Province.. Yüzüncü yil Úniversitesi Veterinary Fakültesi Dergisi 23(1), 1–4.
  58. Kuttler KL. World-Wide impact of babesiosis, in Babesiosis of domestic animals and man. CRC Press p. 1–22.
  59. Kuttler KL, Goff WL, Gipson CA, Blackburn BO. Serologic response of Babesia equi-infected horses as measured by complement-fixation and indirect fluorescent antibody tests.. Vet Parasitol 1988 Jan;26(3-4):199-205.
    pubmed: 3279699doi: 10.1016/0304-4017(88)90088-xgoogle scholar: lookup
  60. Laus F, Veronesi F, Passamonti F, Paggi E, Cerquetella M, Hyatt D, Tesei B, Fioretti DP. Prevalence of tick borne pathogens in horses from Italy.. J Vet Med Sci 2013;75(6):715-20.
    pubmed: 23328633doi: 10.1292/jvms.12-0449google scholar: lookup
  61. nMAGRAMA. 2013. nhttp://www.mapama.gob.es/es/ganaderia/temas/produccion-y-mercados-ganaderos/indicadoreseconomicossectorequino2015_tcm7-386080.pdfn(accessed 27 February 2017).
  62. Mahmoud MS, El-Ezz NT, Abdel-Shafy S, Nassar SA, El Namaky AH, Khalil WK, Knowles D, Kappmeyer L, Silva MG, Suarez CE. Assessment of Theileria equi and Babesia caballi infections in equine populations in Egypt by molecular, serological and hematological approaches.. Parasit Vectors 2016 May 4;9:260.
    pmc: PMC4857240pubmed: 27146413doi: 10.1186/s13071-016-1539-9google scholar: lookup
  63. Malekifard F, Tavassoli M, Yakhchali M, Darvishzadeh R. Detection of Theileria equi and Babesia caballi using microscopic and molecular methods in horses in suburb of Urmia, Iran.. Vet Res Forum 2014 Spring;5(2):129-33.
    pmc: PMC4279624pubmed: 25568706
  64. Mans BJ, Pienaar R, Latif AA. A review of Theileria diagnostics and epidemiology.. Int J Parasitol Parasites Wildl 2015 Apr;4(1):104-18.
    pmc: PMC4356873pubmed: 25830110doi: 10.1016/j.ijppaw.2014.12.006google scholar: lookup
  65. Mehlhorn H, Schein E. Redescription of Babesia equi Laveran, 1901 as Theileria equi Mehlhorn, Schein 1998.. Parasitol Res 1998 Jun;84(6):467-75.
    pubmed: 9660136doi: 10.1007/s004360050431google scholar: lookup
  66. Montes-Cortés MG, Fernández-García JL, Martínez-Estéllez MÁH. Genetic Variation of the β-tubulin Gene of Babesia caballi Strains.. J Arthropod Borne Dis 2017 Sep;11(3):344-353.
    pmc: PMC5758630pubmed: 29322051
  67. Moretti A, Mangili V, Salvatori R, Maresca C, Scoccia E, Torina A, Moretta I, Gabrielli S, Tampieri MP, Pietrobelli M. Prevalence and diagnosis of Babesia and Theileria infections in horses in Italy: a preliminary study.. Vet J 2010 Jun;184(3):346-50.
    pubmed: 19394253doi: 10.1016/j.tvjl.2009.03.021google scholar: lookup
  68. Motloang MY, Thekisoe OM, Alhassan A, Bakheit M, Motheo MP, Masangane FE, Thibedi ML, Inoue N, Igarashi I, Sugimoto C, Mbati PA. Prevalence of Theileria equi and Babesia caballi infections in horses belonging to resource-poor farmers in the north-eastern Free State Province, South Africa.. Onderstepoort J Vet Res 2008 Jun;75(2):141-6.
    pubmed: 18788207doi: 10.4102/ojvr.v75i2.12google scholar: lookup
  69. Mujica FF, Perrone T, Forlano M, Coronado A, Meléndez RD, Barrios N, Alvarez R, Granda F. Serological prevalence of Babesia caballi and Theileria equi in horses of Lara State, Venezuela.. Vet Parasitol 2011 May 31;178(1-2):180-3.
    pubmed: 21273001doi: 10.1016/j.vetpar.2010.12.036google scholar: lookup
  70. Munkhjargal T, Sivakumar T, Battsetseg B, Nyamjargal T, Aboulaila M, Purevtseren B, Bayarsaikhan D, Byambaa B, Terkawi MA, Yokoyama N, Igarashi I. Prevalence and genetic diversity of equine piroplasms in Tov province, Mongolia.. Infect Genet Evol 2013 Jun;16:178-85.
    pubmed: 23416256doi: 10.1016/j.meegid.2013.02.005google scholar: lookup
  71. Nagore D, García-Sanmartín J, García-Pérez AL, Juste RA, Hurtado A. Detection and identification of equine Theileria and Babesia species by reverse line blotting: epidemiological survey and phylogenetic analysis.. Vet Parasitol 2004 Aug 13;123(1-2):41-54.
    pubmed: 15265570doi: 10.1016/j.vetpar.2004.04.010google scholar: lookup
  72. Nicolaiewsky TB, Richter MF, Lunge VR, Cunha CW, Delagostin O, Ikuta N, Fonseca AS, da Silva SS, Ozaki LS. Detection of Babesia equi (Laveran, 1901) by nested polymerase chain reaction.. Vet Parasitol 2001 Oct 31;101(1):9-21.
    pubmed: 11587829doi: 10.1016/s0304-4017(01)00471-xgoogle scholar: lookup
  73. . Equine piroplasmosis, in Manual of diagnostic test and vaccines for terrestrial animals. (Chapter 12.7).
  74. Olivera M, García F. Equine babesiosis seroprevalence in thoroughbred racing horse farms from Aragua and Carabobo State, Venezuela.. Revista de la Facultad de Ciencias Veterinarias de la Universidad Central de Venezuela 42(1–2), 3–13.
  75. Posada-Guzmán MF, Dolz G, Romero-Zúñiga JJ, Jiménez-Rocha AE. Detection of Babesia caballi and Theileria equi in Blood from Equines from Four Indigenous Communities in Costa Rica.. Vet Med Int 2015;2015:236278.
    pmc: PMC4663365pubmed: 26649225doi: 10.1155/2015/236278google scholar: lookup
  76. Prochno HC, Scorsin LM, De Melo FR, Baldani CD, Falbo MK, de Aquino LC, Lemos KR. Seroprevalence rates of antibodies against Theileria equi in team roping horses from central-western region of Paraná.. Rev Bras Parasitol Vet 2014 Mar;23(1):85-9.
    pubmed: 24728366doi: 10.1590/s1984-29612014012google scholar: lookup
  77. Qablan MA, Oborník M, Petrželková KJ, Sloboda M, Shudiefat MF, Hořín P, Lukeš J, Modrý D. Infections by Babesia caballi and Theileria equi in Jordanian equids: epidemiology and genetic diversity.. Parasitology 2013 Aug;140(9):1096-103.
    pubmed: 23673249doi: 10.1017/s0031182013000486google scholar: lookup
  78. Rampersad J, Cesar E, Campbell MD, Samlal M, Ammons D. A field evaluation of PCR for the routine detection of Babesia equi in horses.. Vet Parasitol 2003 May 30;114(2):81-7.
    pubmed: 12781470doi: 10.1016/s0304-4017(03)00129-8google scholar: lookup
  79. Rapoport A, Aharonson-Raz K, Berlin D, Tal S, Gottlieb Y, Klement E, Steinman A. Molecular characterization of the Babesia caballi rap-1 gene and epidemiological survey in horses in Israel.. Infect Genet Evol 2014 Apr;23:115-20.
    pubmed: 24524984doi: 10.1016/j.meegid.2014.01.033google scholar: lookup
  80. Ribeiro AJ, Cardoso L, Maia JM, Coutinho T, Cotovio M. Prevalence of Theileria equi, Babesia caballi, and Anaplasma phagocytophilum in horses from the north of Portugal.. Parasitol Res 2013 Jul;112(7):2611-7.
    pubmed: 23591484doi: 10.1007/s00436-013-3429-9google scholar: lookup
  81. Rosales R, Rangel-Rivas A, Escalona A, Jordan LS, Gonzatti MI, Aso PM, Perrone T, Silva-Iturriza A, Mijares A. Detection of Theileria equi and Babesia caballi infections in Venezuelan horses using Competitive-Inhibition ELISA and PCR.. Vet Parasitol 2013 Sep 1;196(1-2):37-43.
    pubmed: 23582233doi: 10.1016/j.vetpar.2013.02.004google scholar: lookup
  82. Ros-García A, M'ghirbi Y, Hurtado A, Bouattour A. Prevalence and genetic diversity of piroplasm species in horses and ticks from Tunisia.. Infect Genet Evol 2013 Jul;17:33-7.
    pubmed: 23542456doi: 10.1016/j.meegid.2013.03.038google scholar: lookup
  83. Rothschild CM, Knowles DP. Equine Piroplasmosis, in Equine Infectious Diseases. Saunders Elsevier p. 465–473.
  84. Rüegg SR, Torgerson P, Deplazes P, Mathis A. Age-dependent dynamics of Theileria equi and Babesia caballi infections in southwest Mongolia based on IFAT and/or PCR prevalence data from domestic horses and ticks.. Parasitology 2007 Jul;134(Pt 7):939-47.
    pubmed: 17306055doi: 10.1017/s0031182007002405google scholar: lookup
  85. Salim BO, Hassan SM, Bakheit MA, Alhassan A, Igarashi I, Karanis P, Abdelrahman MB. Diagnosis of Babesia caballi and Theileria equi infections in horses in Sudan using ELISA and PCR.. Parasitol Res 2008 Oct;103(5):1145-50.
    pubmed: 18618143doi: 10.1007/s00436-008-1108-zgoogle scholar: lookup
  86. Salim B, Bakheit MA, Kamau J, Sugimoto C. Current status of equine piroplasmosis in the Sudan.. Infect Genet Evol 2013 Jun;16:191-9.
    pubmed: 23485745doi: 10.1016/j.meegid.2013.02.008google scholar: lookup
  87. Seo MG, Yun SH, Choi SK, Cho GJ, Park YS, Kwon OD, Cho KH, Kim TH, Jeong KS, Park SJ, Kwon YS, Kwak D. Seroprevalence of equine piroplasms in the Republic of Korea.. Vet Parasitol 2011 Jun 30;179(1-3):224-6.
    pubmed: 21429670doi: 10.1016/j.vetpar.2011.02.020google scholar: lookup
  88. Seo MG, Yun SH, Choi SK, Cho GJ, Park YS, Cho KH, Kwon OD, Kwak D. Molecular and phylogenetic analysis of equine piroplasms in the Republic of Korea.. Res Vet Sci 2013 Jun;94(3):579-83.
    pubmed: 23415067doi: 10.1016/j.rvsc.2013.01.014google scholar: lookup
  89. Sgorbini M, Bonelli F, Nardoni S, Rocchigiani G, Corazza M, Mancianti F. Seroprevalence and molecular analysis of Babesia caballi and Theileria equi from Central Italy during a 10-year period.. Journal of Equine Veterinary Science 35, 865–868.
  90. Sevinc F, Maden M, Kumas C, Sevinc M, Ekici OD. A comparative study on the prevalence of Theileria equi and Babesia caballi infections in horse sub-populations in Turkey.. Vet Parasitol 2008 Oct 1;156(3-4):173-7.
    pubmed: 18672330doi: 10.1016/j.vetpar.2008.06.006google scholar: lookup
  91. Shkap V, Cohen I, Leibovitz B, Savitsky, Pipano E, Avni G, Shofer S, Giger U, Kappmeyer L, Knowles D. Seroprevalence of Babesia equi among horses in Israel using competitive inhibition ELISA and IFA assays.. Vet Parasitol 1998 Apr 30;76(4):251-9.
    pubmed: 9650862doi: 10.1016/s0304-4017(97)00228-8google scholar: lookup
  92. Sigg L, Gerber V, Gottstein B, Doherr MG, Frey CF. Seroprevalence of Babesia caballi and Theileria equi in the Swiss horse population.. Parasitol Int 2010 Sep;59(3):313-7.
    pubmed: 20178863doi: 10.1016/j.parint.2010.02.005google scholar: lookup
  93. Sloboda M, Jirků M, Lukešová D, Qablan M, Batsukh Z, Fiala I, Hořín P, Modrý D, Lukeš J. A survey for piroplasmids in horses and Bactrian camels in North-Eastern Mongolia.. Vet Parasitol 2011 Jun 30;179(1-3):246-9.
    pubmed: 21402446doi: 10.1016/j.vetpar.2011.01.064google scholar: lookup
  94. Steinman A, Zimmerman T, Klement E, Lensky IM, Berlin D, Gottlieb Y, Baneth G. Demographic and environmental risk factors for infection by Theileria equi in 590 horses in Israel.. Vet Parasitol 2012 Jul 6;187(3-4):558-62.
    pubmed: 22293151doi: 10.1016/j.vetpar.2012.01.018google scholar: lookup
  95. Sumbria D, Singla LD, Kumar S, Sharma A, Dahiya RK, Setia R. Spatial distribution, risk factors and haemato-biochemical alterations associated with Theileria equi infected equids of Punjab (India) diagnosed by indirect ELISA and nested PCR.. Acta Trop 2016 Mar;155:104-12.
    pubmed: 26747007doi: 10.1016/j.actatropica.2015.12.020google scholar: lookup
  96. Sumbria D, Das Singla L, Sharma A. Theileria equi and Babesia caballi infection of equids in Punjab, India: a serological and molecular survey.. Trop Anim Health Prod 2016 Jan;48(1):45-52.
    pubmed: 26387094doi: 10.1007/s11250-015-0917-1google scholar: lookup
  97. Teglas M, Matern E, Lein S, Foley P, Mahan SM, Foley J. Ticks and tick-borne disease in Guatemalan cattle and horses.. Vet Parasitol 2005 Jul 15;131(1-2):119-27.
    pubmed: 15936147doi: 10.1016/j.vetpar.2005.04.033google scholar: lookup
  98. Tenter AM, Otte MJ, Gonzalez CA, Abuabara Y. Prevalence of piroplasmosis in equines in the Colombian province of Cordoba.. Trop Anim Health Prod 1988 May;20(2):93-8.
    pubmed: 3041656doi: 10.1007/bf02242234google scholar: lookup
  99. Thrusfield M, Ortega C, de Blas I, Noordhuizen JP, Frankena K. WIN EPISCOPE 2.0: improved epidemiological software for veterinary medicine.. Vet Rec 2001 May 5;148(18):567-72.
    pubmed: 11370882doi: 10.1136/vr.148.18.567google scholar: lookup
  100. Vieira TS, Vieira RF, Finger MA, Nascimento DA, Sicupira PM, Dutra LH, Deconto I, Barros-Filho IR, Dornbusch PT, Biondo AW, Vidotto O. Seroepidemiological survey of Theileria equi and Babesia caballi in horses from a rural and from urban areas of Paraná State, southern Brazil.. Ticks Tick Borne Dis 2013 Dec;4(6):537-41.
    pubmed: 24209493doi: 10.1016/j.ttbdis.2013.07.005google scholar: lookup
  101. Wang M, Guo W, Igarashi I, Xuan X, Wang X, Xiang W, Jia H. Epidemiological investigation of equine piroplasmosis in China by enzyme-linked immunosorbent assays.. J Vet Med Sci 2014 Apr;76(4):549-52.
    pmc: PMC4064140pubmed: 24292247doi: 10.1292/jvms.13-0477google scholar: lookup
  102. Wise LN, Kappmeyer LS, Mealey RH, Knowles DP. Review of equine piroplasmosis.. J Vet Intern Med 2013 Nov-Dec;27(6):1334-46.
    pubmed: 24033559doi: 10.1111/jvim.12168google scholar: lookup
  103. Xu Y, Zhang S, Huang X, Bayin C, Xuan X, Igarashi I, Fujisaki K, Kabeya H, Maruyama S, Mikami T. Seroepidemiologic studies on Babesia equi and Babesia caballi infections in horses in Jilin province of China.. J Vet Med Sci 2003 Sep;65(9):1015-7.
    pubmed: 14532697doi: 10.1292/jvms.65.1015google scholar: lookup
  104. Xuan X, Nagai A, Battsetseg B, Fukumoto S, Makala LH, Inoue N, Igarashi I, Mikami T, Fujisaki K. Diagnosis of equine piroplasmosis in Brazil by serodiagnostic methods with recombinant antigens.. J Vet Med Sci 2001 Oct;63(10):1159-60.
    pubmed: 11714038doi: 10.1292/jvms.63.1159google scholar: lookup
  105. Xuan X, Chahan B, Huang X, Yokoyama N, Makala LH, Igarashi I, Fujisaki K, Maruyama S, Sakai T, Mikami T. Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens.. Vet Parasitol 2002 Sep 10;108(2):179-82.
    pubmed: 12208045doi: 10.1016/s0304-4017(02)00193-0google scholar: lookup

Citations

This article has been cited 16 times.
  1. Giubega S, Ilie MS, Luca I, Florea T, Dreghiciu C, Oprescu I, Morariu S, Dărăbuș G. Seroprevalence of Anti-Theileria equi Antibodies in Horses from Three Geographically Distinct Areas of Romania. Pathogens 2022 Jun 9;11(6).
    doi: 10.3390/pathogens11060669pubmed: 35745523google scholar: lookup
  2. Villa L, Gazzonis AL, Allievi C, De Maria C, Persichetti MF, Caracappa G, Zanzani SA, Manfredi MT. Seroprevalence of Tick-Borne Infections in Horses from Northern Italy. Animals (Basel) 2022 Apr 12;12(8).
    doi: 10.3390/ani12080999pubmed: 35454246google scholar: lookup
  3. Bravo-Barriga D, Serrano-Aguilera FJ, Barrasa-Rita R, Habela MÁ, Chacón RB, Ezquerra LJ, Martín-Cuervo M. Effects of Competitive ELISA-Positive Results of Piroplasmosis on the Performance of Endurance Horses. Animals (Basel) 2022 Mar 3;12(5).
    doi: 10.3390/ani12050637pubmed: 35268210google scholar: lookup
  4. Idoko IS, Edeh RE, Adamu AM, Machunga-Mambula S, Okubanjo OO, Balogun EO, Adamu S, Johnson W, Kappmeyer L, Mousel M, Ueti MW. Molecular and Serological Detection of Piroplasms in Horses from Nigeria. Pathogens 2021 Apr 23;10(5).
    doi: 10.3390/pathogens10050508pubmed: 33922468google scholar: lookup
  5. Fuehrer HP, Alho AM, Kayikci FN, Shahi Barogh B, Rosa H, Tomás J, Rocha H, Harl J, Madeira de Carvalho L. Survey of Zoonotic and Non-zoonotic Vector-Borne Pathogens in Military Horses in Lisbon, Portugal. Front Vet Sci 2020;7:591943.
    doi: 10.3389/fvets.2020.591943pubmed: 33195629google scholar: lookup
  6. Tirosh-Levy S, Gottlieb Y, Fry LM, Knowles DP, Steinman A. Twenty Years of Equine Piroplasmosis Research: Global Distribution, Molecular Diagnosis, and Phylogeny. Pathogens 2020 Nov 8;9(11).
    doi: 10.3390/pathogens9110926pubmed: 33171698google scholar: lookup
  7. Onyiche TE, Taioe MO, Molefe NI, Biu AA, Luka J, Omeh IJ, Yokoyama N, Thekisoe O. Equine piroplasmosis: an insight into global exposure of equids from 1990 to 2019 by systematic review and meta-analysis. Parasitology 2020 Nov;147(13):1411-1424.
    doi: 10.1017/S0031182020001407pubmed: 32741382google scholar: lookup
  8. Onyiche TE, Taioe MO, Ogo NI, Sivakumar T, Biu AA, Mbaya AW, Xuan X, Yokoyama N, Thekisoe O. Molecular evidence of Babesia caballi and Theileria equi in equines and ticks in Nigeria: prevalence and risk factors analysis. Parasitology 2020 Sep;147(11):1238-1248.
    doi: 10.1017/S0031182020000992pubmed: 32605687google scholar: lookup
  9. Onyiche TE, Suganuma K, Igarashi I, Yokoyama N, Xuan X, Thekisoe O. A Review on Equine Piroplasmosis: Epidemiology, Vector Ecology, Risk Factors, Host Immunity, Diagnosis and Control. Int J Environ Res Public Health 2019 May 16;16(10).
    doi: 10.3390/ijerph16101736pubmed: 31100920google scholar: lookup
  10. Duaso J, Perez-Ecija A, Navarro A, Martínez E, De Las Heras A, Mendoza FJ. True Prevalence and Seroprevalence of Piroplasmosis in Horses in Southwestern Europe. Animals (Basel) 2025 Jul 11;15(14).
    doi: 10.3390/ani15142047pubmed: 40723509google scholar: lookup
  11. Villa L, Cafiso A, Cialini C, Olivieri E, Allievi C, Pintore E, Garippa G, Manfredi MT, Bazzocchi C. Serological and molecular insights into tick-borne pathogens in wild donkeys from an unexplored Mediterranean nature reserve. Curr Res Parasitol Vector Borne Dis 2025;7:100267.
    doi: 10.1016/j.crpvbd.2025.100267pubmed: 40503032google scholar: lookup
  12. Giubega S, Ilie MS, Morariu S, Imre M, Dreghiciu C, Rugea T, Ivascu S, Simion G, Dărăbuș G. Molecular Investigations of Babesia caballi from Clinically Healthy Horses in Southwestern Romania. Vet Sci 2024 Nov 27;11(12).
    doi: 10.3390/vetsci11120600pubmed: 39728940google scholar: lookup
  13. Axt CW, Springer A, von Luckner J, Naucke TJ, Müller E, Strube C, Schäfer I. [Equine piroplasmosis: Case descriptions and overview of the epidemiological situation in Europe with focus on Germany]. Tierarztl Prax Ausg G Grosstiere Nutztiere 2025 Feb;53(1):49-58.
    doi: 10.1055/a-2457-5516pubmed: 39631762google scholar: lookup
  14. Altay K, Erol U, Sahin OF, Ulucesme MC, Aytmirzakizi A, Aktas M. Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi. Front Vet Sci 2024;11:1359974.
    doi: 10.3389/fvets.2024.1359974pubmed: 38746933google scholar: lookup
  15. Axt CW, Springer A, Strube C, Jung C, Naucke TJ, Müller E, Schäfer I. Molecular and Serological Detection of Vector-Borne Pathogens Responsible for Equine Piroplasmosis in Europe between 2008 and 2021. Microorganisms 2024 Apr 17;12(4).
    doi: 10.3390/microorganisms12040816pubmed: 38674760google scholar: lookup
  16. Coluccia P, Gizzarelli M, Scicluna MT, Manna G, Foglia Manzillo V, Buono F, Auletta L, Palumbo V, Pasolini MP. A cross-sectional study on performance evaluation in Italian standardbred horses' real-time PCR-positive for Theileria equi. BMC Vet Res 2024 Mar 5;20(1):79.
    doi: 10.1186/s12917-024-03908-0pubmed: 38443906google scholar: lookup
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