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Veterinary world2016; 9(3); 251-255; doi: 10.14202/vetworld.2016.251-255

Serum muscle-derived enzymes response during show jumping competition in horse.

Abstract: The effect of two jumping competitions, performed in two consecutive weekends, on serum creatine phosphokinase (CPK), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH), urea, creatinine (CREA) concentrations were evaluated in 12 healthy jumper horses. Methods: Blood sampling was performed before the 1(st) day of competition (T0), at the end of each show (J1, J2), on the day after the competition (T1); the same sampling plan was followed during the second weekend (J3, J4 and T2). Results: One-way repeated measures analysis of variance showed an increase in CPK at J1 and J2 respect to T0 and at J3 and J4 respect to all other time points (p<0.05). LDH activity showed an increase at J2 respect to T0, at J3 respect to T0, J1, J2 and at J4 respect to all other time points (p<0.05). AST values increased at J1 and J2 respect to T0 (p<0.05). A significant increase of CREA was found at J3 respect to T0, T1 and J1 and at J4 respect to all other time points (p<0.05). A decrease in serum urea levels was found at J1 respect to T0, at J2 and J4 respect to T0 and T1; at T2 respect to T0 (p<0.05). A positive correlation between urea/CPK (p=0.0042, r(2)=0.030), LDH/CPK (p<0.0001, r(2)=0.535), CREA/LDH (p<0.0001, r(2)=0.263), CREA/CPK (p<0.0001, r(2)=0.496) was observed. Conclusions: Our results suggest that 5 days recovery period between the two consecutive competition weekends is insufficient to allow muscle recovery and avoid potential additional stress. The findings obtained in this study improve the knowledge about metabolic changes occurring in athlete horse during the competition to identify muscle alterations following show jumping competitions.
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Publication Date: 2016-03-08 PubMed ID: 27057107PubMed Central: PMC4823284DOI: 10.14202/vetworld.2016.251-255Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the effect of jumping competitions on serum creatine phosphokinase (CPK), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), urea, and creatinine concentrations in horse serum. The results indicate that the 5-day recovery period between two consecutive competition weekends is insufficient for muscle recovery and could potentially lead to additional stress.

Objective and Methodology

  • The study aimed to evaluate the impact of show jumping competitions on serum levels of key muscle-associated enzymes in horses. The enzymes of focus were CPK, AST, LDH as well as urea and CREA concentrations.
  • The research involved 12 healthy jumper horses, who participated in two jumping competitions on two consecutive weekends.
  • Blood samples were taken at multiple points: before the first day of competition, at the end of each show, and on the day after the competition. This same process was repeated for the second weekend of competitions.

Findings

  • Following the jumps (J1 and J2), it was found that the CPK levels increased compared to T0, and at J3 and J4 compared to all other time points.
  • There was a noted increase in LDH activity at J2 compared to T0 and at J3 and J4 compared to T0, J1, and J2.
  • AST values showed an increase at J1 and J2 compared to T0.
  • A significant increase in CREA was found at J3 compared to T0, T1, and J1 and subsequently at J4 compared to all other time points.
  • A decrease in serum urea levels was identified at J1 compared to T0, at J2 and J4 compared to T0 and T1, and at T2 compared to T0.
  • Positive correlations were observed between urea/CPK, LDH/CPK, CREA/LDH, and CREA/CPK.

Conclusion

  • The study concluded that the five-day recovery period between two consecutive jumping competitions was insufficient for full muscle recovery. This could potentially lead to additional stress in competitive horses.
  • This research aids in the understanding of metabolic changes occurring in athlete horses during competitions and can help identify muscle alterations following intense activities like show jumping.

Cite This Article

APA
Assenza A, Marafioti S, Congiu F, Giannetto C, Fazio F, Bruschetta D, Piccione G. (2016). Serum muscle-derived enzymes response during show jumping competition in horse. Vet World, 9(3), 251-255. https://doi.org/10.14202/vetworld.2016.251-255

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 9
Issue: 3
Pages: 251-255

Researcher Affiliations

Assenza, Anna
  • Department of Cognitive Science, Education and Cultural Studies, University of Messina, Via Concezione n 6-8, 98122, Messina, Italy.
Marafioti, Simona
  • Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168, Messina, Italy.
Congiu, Fulvio
  • Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168, Messina, Italy.
Giannetto, Claudia
  • Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168, Messina, Italy.
Fazio, Francesco
  • Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168, Messina, Italy.
Bruschetta, Daniele
  • Department of Biomedical Sciences and Morpho-functional Imaging, University of Messina, Via Consolare Valeria 1, Messina, Italy.
Piccione, Giuseppe
  • Department of Veterinary Sciences, University of Messina, Polo Universitario dell'Annunziata, 98168, Messina, Italy.

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Citations

This article has been cited 5 times.
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  2. Wonghanchao T, Huangsaksri O, Sanigavatee K, Poochipakorn C, Chanprame S, Wongkosoljit S, Chotiyothin W, Rattanayanon N, Kiawwan R, Chanda M. Autonomic regulation in athletic horses repetitively participating in two novice jumping classes on consecutive days. Front Vet Sci 2024;11:1456733.
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  3. Kirsch K, Fercher C, Horstmann S, von Reitzenstein C, Augustin J, Lagershausen H. Monitoring Performance in Show Jumping Horses: Validity of Non-specific and Discipline-specific Field Exercise Tests for a Practicable Assessment of Aerobic Performance. Front Physiol 2021;12:818381.
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  4. Buckley P, Buckley DJ, Freire R, Hughes KJ. Pre-race and race management impacts serum muscle enzyme activity in Australian endurance horses. Equine Vet J 2022 Sep;54(5):895-904.
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  5. Miglio A, Gavazza A, Siepi D, Bagaglia F, Misia A, Antognoni MT. Hematological and Biochemical Reference Intervals for 5 Adult Hunting Dog Breeds Using a Blood Donor Database. Animals (Basel) 2020 Jul 16;10(7).
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