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Home / Equine Research Bank / Vet World / Species determination using the red blood cells morphometry ...
Veterinary world2016; 9(9); 960-963; doi: 10.14202/vetworld.2016.960-963

Species determination using the red blood cells morphometry in domestic animals.

Abstract: This investigation is placed in the context of continuity of a preliminary study already published; it was conducted in cattle, sheep, goats, horses, and dogs; the main aim is to reveal and develop criteria for the animal species determination based on the morphometric parameters of red blood cells. Methods: Blood samples were taken from the jugular vein; and the smears were confectioned on slides immediately after the blood collection and stained according to the May-Gründwald Giemsa method. For the morphometric study, three parameters were considered which are: The diameter, the circumference, and the surface of erythrocytes; and measurements were achieved using the OPTIKATM Vision Pro software. Statistical analysis was performed by both analysis of variances and Student's t analytical tests. Results: The recorded data showed that the three morphometric parameters of red blood cells are higher in dogs followed, respectively, by those of horses, cattle, and sheep, whereas, the goats have the lowest ones. In addition, the obtained results allowed us to propose new reference values for the circumference and the surface of erythrocyte in considered species. Conclusions: This investigation permit concluding that from a drop of blood it is possible to characterize the different animal species, taking into account the diameter, the circumference, and the surface of erythrocytes.
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Publication Date: 2016-09-11 PubMed ID: 27733796PubMed Central: PMC5057034DOI: 10.14202/vetworld.2016.960-963Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research explores the possibility of determining animal species through the analysis of red blood cell morphology in domestic animals like cattle, sheep, goats, horses, and dogs.

Research Methodology

  • As part of the study, blood samples were collected from the jugular vein of various animals including cattle, horses, sheep, goats, and dogs.
  • To maintain sample integrity, smears were prepared quickly on slides post-collection and stained using the May-Gründwald Giemsa method.
  • The morphology of the red blood cells was studied considering three parameters – diameter, circumference, and surface area. These measurements were accomplished using the OPTIKATM Vision Pro software.
  • Statistical analyses of the data obtained were performed employing analysis of variances and Student’s t analytic tests.

Research Findings

  • The data recorded highlighted that the morphometric parameters of red blood cells are highest in dogs, followed by horses, cattle, sheep, and goats, which displayed the lowest measurements.
  • The outcomes of the study also provided the opportunity to suggest new reference values for the circumference and surface area of red blood cells in the specified animal species.

Research Conclusion

  • The research concluded that characterizing different animal species can be accomplished by analyzing a single drop of blood, primarily looking at differences in the diameter, circumference, and surface of their red blood cells.
  • This investigation is a continuation of a previous preliminary study and adds to the understanding of animal species determination based on the shape and size of their red blood cells.

Cite This Article

APA
Adili N, Melizi M, Belabbas H. (2016). Species determination using the red blood cells morphometry in domestic animals. Vet World, 9(9), 960-963. https://doi.org/10.14202/vetworld.2016.960-963

Publication

Veterinary world
ISSN: 0972-8988
NlmUniqueID: 101504872
Country: India
Language: English
Volume: 9
Issue: 9
Pages: 960-963

Researcher Affiliations

Adili, Nezar
  • Department of Veterinary Medicine, Institute of Veterinary Sciences and Agricultural Sciences, University of Batna 1, Batna 05000, Algeria.
Melizi, Mohamed
  • Department of Veterinary Medicine, Institute of Veterinary Sciences and Agricultural Sciences, University of Batna 1, Batna 05000, Algeria.
Belabbas, Hadj
  • Department of Microbiology and Biochemistry, Faculty of Sciences, University of Mohamed BOUDIAF, M'sila 28000, Algeria.

References

This article includes 19 references
  1. ADAMS GC. A technique for the measurement of erythrocyte diameters.. J Clin Pathol 1954 Feb;7(1):76-8.
    pmc: PMC1023705pubmed: 13143111doi: 10.1136/jcp.7.1.76google scholar: lookup
  2. Tood J C. Clinical Diagnosis by Laboratory Methods. Philadelphia, PA U S,A: Lippincott, Williams and Wilkins; 1979.
  3. Wood D, Quiroz-Rocha G F. Normal hematology of cattle. Schalm's Veterinary Hematology 6th ed. Wiley-Blackwell Publishing Ltd., Ames, Iowa U S,A; 2010. pp. 829–835.
  4. Byers S R, Kramer J W. Normal hematology of sheep and goats. Schalm's Veterinary Hematology 6th ed. Wiley-Blackwell Publishing Ltd., Ames, Iowa U S,A; 2010. pp. 836–842.
  5. Lording PM. Erythrocytes.. Vet Clin North Am Equine Pract 2008 Aug;24(2):225-37, v.
    pubmed: 18652953doi: 10.1016/j.cveq.2008.04.002google scholar: lookup
  6. Reagan W J, Irizarryrovira A R, Denicola D B. Veterinary Hematology Atlas Common Domestic and Non-domestic Species. 2nd ed. Wiley-Blackwell Publishing Ltd., Ames, Iowa U S,A: 2008. Normal red blood cell morphology; pp. 13–16.
  7. Grondin T M, Dewitt S F. Normal hematology of the horse and donkey. Schalm's Veterinary Hematology 6th ed. Wiley- Blackwell Publishing Ltd., Ames, Iowa U S,A; 2010. pp. 821–828.
  8. Rizzi T E, Meinkoth J H, Clinkenbeard K D. Normal hematology of the dog. Schalm's Veterinary Hematology 6th ed. Ames, Iowa U S,A: Wiley-Blackwell Publishing Ltd; 2010. pp. 799–810.
  9. Meinkoth J H, Clinkenbeard K D. Normal hematology of the dog. Schalm's Veterinary Hematology 5th ed. Philadelphia, PA U S,A: Lippincott, Williams and Wilkins; 2000. pp. 1057–1063.
  10. Brun-Hansen HC, Kampen AH, Lund A. Hematologic values in calves during the first 6 months of life.. Vet Clin Pathol 2006 Jun;35(2):182-7.
    pubmed: 16783710doi: 10.1111/j.1939-165x.2006.tb00111.xgoogle scholar: lookup
  11. Malikides N, Mollison PJ, Reid SW, Murray M. Haematological responses of repeated large volume blood collection in the horse.. Res Vet Sci 2000 Jun;68(3):275-8.
    pubmed: 10877975doi: 10.1053/rvsc.2000.0376google scholar: lookup
  12. Taylor F G, Brazil T J, Hillyer M H. Diagnostic Techniques in Equine Medicine. 2nd ed. Philadelphia, PA U S,A: W.B. Saunders Company, Elsevier Limited; 2010.
  13. Adili N, Melizi M. Preliminary study of the influence of red blood cells morphometry on the species determinism of domestic animals. Vet. World 2014;7(4):219–223.
  14. Allison RW, Meinkoth JH. Hematology without the numbers: in-clinic blood film evaluation.. Vet Clin North Am Small Anim Pract 2007 Mar;37(2):245-66, vi.
    pubmed: 17336674doi: 10.1016/j.cvsm.2006.10.002google scholar: lookup
  15. DeNicola DB. Advances in hematology analyzers.. Top Companion Anim Med 2011 May;26(2):52-61.
    pubmed: 21596345doi: 10.1053/j.tcam.2011.02.001google scholar: lookup
  16. Gavazza A, Ricci M, Brettoni M, Gugliucci B, Pasquini A, Rispoli D, Bernabò N, Lubas G. Retrospective and Prospective Investigations about "Quatrefoil" Erythrocytes in Canine Blood Smears.. Vet Med Int 2014;2014:409573.
    pmc: PMC3913368pubmed: 24511413doi: 10.1155/2014/409573google scholar: lookup
  17. Houwen B. Blood film preparation and staining procedures. Lab. Hematol. 2000:1–7.
  18. Harvey J W. Atlas of Veterinary Hematology: Blood and Bone Marrow of Domestic Animals. Philadelphia, PA U S,A: W.B. Saunders Company; 2001.
  19. Ledieu D. Prélèvements en cytologie. Biologie Clinique: Encyclopédie Vétérinaire Elsevier SAS; 2003. p. 0030.

Citations

This article has been cited 11 times.
  1. Matsuura K, Takata K. Blood Cell Separation Using Polypropylene-Based Microfluidic Devices Based on Deterministic Lateral Displacement. Micromachines (Basel) 2023 Jan 17;14(2).
    doi: 10.3390/mi14020238pubmed: 36837938google scholar: lookup
  2. Mistek-Morabito E, Lednev IK. Discrimination between human and animal blood by attenuated total reflection Fourier transform-infrared spectroscopy. Commun Chem 2020 Dec 10;3(1):178.
    doi: 10.1038/s42004-020-00424-8pubmed: 36703343google scholar: lookup
  3. Montoya-Navarrete AL, Guerrero-Barrera AL, Quezada-Tristán T, Valdivia-Flores AG, Cano-Rábano MJ. Red blood cells morphology and morphometry in adult, senior, and geriatricians dogs by optical and scanning electron microscopy. Front Vet Sci 2022;9:998438.
    doi: 10.3389/fvets.2022.998438pubmed: 36439358google scholar: lookup
  4. Prata JC, Silva ALP, da Costa JP, Dias-Pereira P, Carvalho A, Fernandes AJS, da Costa FM, Duarte AC, Rocha-Santos T. Microplastics in Internal Tissues of Companion Animals from Urban Environments. Animals (Basel) 2022 Aug 4;12(15).
    doi: 10.3390/ani12151979pubmed: 35953968google scholar: lookup
  5. Smith JS, Viall AK, Breuer RM, Walton RA, Plummer PJ, Griffith RW, Kreuder AJ. Preliminary Investigation of Bovine Whole Blood Xenotransfusion as a Therapeutic Modality for the Treatment of Anemia in Goats. Front Vet Sci 2021;8:637988.
    doi: 10.3389/fvets.2021.637988pubmed: 33748213google scholar: lookup
  6. O'Connor T, Anand A, Andemariam B, Javidi B. Deep learning-based cell identification and disease diagnosis using spatio-temporal cellular dynamics in compact digital holographic microscopy. Biomed Opt Express 2020 Aug 1;11(8):4491-4508.
    doi: 10.1364/BOE.399020pubmed: 32923059google scholar: lookup
  7. Žura Žaja I, Vince S, Poljičak Milas N, Lobpreis IRA, Špoljarić B, Shek Vugrovečki A, Milinković-Tur S, Šimpraga M, Pajurin L, Mikuš T, Vlahović K, Popović M, Špoljarić D. A New Method of Assessing Sheep Red Blood Cell Types from Their Morphology. Animals (Basel) 2019 Dec 12;9(12).
    doi: 10.3390/ani9121130pubmed: 31842441google scholar: lookup
  8. Behrangzade A, Ye SH, Maestas DR Jr, Wagner WR, Vande Geest JP. Improving the hemocompatibility of a porohyperelastic layered vascular graft using luminal reversal microflows. J Mech Behav Biomed Mater 2024 Sep;157:106638.
    doi: 10.1016/j.jmbbm.2024.106638pubmed: 38996626google scholar: lookup
  9. Shah SSH, Elmorsy E, Othman RQA, Syed A, Armaghan SU, Khalid Bokhari SU, Elmorsy ME, Bawadekji A. The Evaluation of Artificial Intelligence Technology for the Differentiation of Fresh Human Blood Cells From Other Species' Blood in the Investigation of Crime Scenes. Cureus 2024 Apr;16(4):e58496.
    doi: 10.7759/cureus.58496pubmed: 38765447google scholar: lookup
  10. Alhaddad H, Alnughaimish A, Alhajeri D, Alhajeri BH. Towards phenotyping adaptive traits in camels: A study of the influence of hypotonic saline solutions on blood cell area. PLoS One 2024;19(3):e0298336.
    doi: 10.1371/journal.pone.0298336pubmed: 38466651google scholar: lookup
  11. ElHamdi S, Sassi L, Rekik M, Dhehibi M, Cheikh M'hamed H, Gharbi M. Conservation agriculture has no significant impact on sheep digestive parasitism. Front Vet Sci 2023;10:1244355.
    doi: 10.3389/fvets.2023.1244355pubmed: 37808117google scholar: lookup
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