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Animals : an open access journal from MDPI2024; 14(8); doi: 10.3390/ani14081242

Stability and Detection Limit of Avian Influenza, Newcastle Disease Virus, and African Horse Sickness Virus on Flinders Technology Associates Card by Conventional Polymerase Chain Reaction.

Abstract: The Flinders Technology Associates (FTA) card, a cotton-based cellulose membrane impregnated with a chaotropic agent, effectively inactivates infectious microorganisms, lyses cellular material, and fixes nucleic acid. The aim of this study is to assess the stability and detection limit of various RNA viruses, especially the avian influenza virus (AIV), Newcastle disease virus (NDV), and African horse sickness virus (AHSV), on the FTA card, which could significantly impact virus storage and transport practices. To achieve this, each virus dilution was inoculated onto an FTA card and stored at room temperature in plastic bags for durations ranging from 1 week to 6 months. Following storage, the target genome was detected using conventional reverse transcription polymerase chain reaction. The present study demonstrated that the detection limit of AIV ranged from 1.17 to 6.17 EID50 values over durations ranging from 1 week to 5 months, while for NDV, it ranged from 2.83 to 5.83 ELD50 over the same duration. Additionally, the detection limit of AHSV was determined as 4.01 PFU for both 1 and 2 weeks, respectively. Based on the demonstrated effectiveness, stability, and safety implications observed in the study, FTA cards are recommended for virus storage and transport, thus facilitating the molecular detection and identification of RNA viral pathogens.
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Publication Date: 2024-04-21 PubMed ID: 38672390PubMed Central: PMC11047397DOI: 10.3390/ani14081242Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research examines the ability of Flinders Technology Associates (FTA) Cards to store and preserve three types of RNA viruses – Avian influenza, Newcastle Disease Virus, and African Horse Sickness Virus. The researchers introduce viruses into the FTA cards and analyze their stability over time.

Research Objective

The study aimed to determine the efficacy of the FTA card in stabilizing and preserving the Avian influenza virus (AIV), Newcastle disease virus (NDV), and African horse sickness virus (AHSV). The researchers sought to identify the detection limit, or the smallest amount of viral presence that can be accurately identified, for each virus over a period of one week to six months.

Methodology

  • The researchers introduced varying amounts of the viruses into the FTA card.
  • These cards were then stored at room temperature inside plastic bags for various periods ranging from one week to six months.
  • Following the storage period, the researchers identified the presence of the virus genome on the card using a technique called reverse transcription polymerase chain reaction.

Findings

The results demonstrate that the detection limit of AIV ranged from 1.17 to 6.17 EID (a measure of virus quantity) depending on the duration of storage; one week to five months.

For NDV, the detection limit ranged from 2.83 to 5.83 ELD, over the same period. The detection limit for AHSV was consistently 4.01 PFU at both one and two weeks.

Implications

From their findings, the researchers recommend FTA cards as a viable method for storing and transporting viruses, given their demonstrated stability and safety for these RNA viral pathogens. This could greatly facilitate the detection and identification of such viruses in a lab environment, potentially improving disease control efforts. The FTA cards do not only inactivate the viruses but also preserve their genetic material, allowing for conclusive identification.

Cite This Article

APA
Taesuji M, Rattanamas K, Yim PB, Ruenphet S. (2024). Stability and Detection Limit of Avian Influenza, Newcastle Disease Virus, and African Horse Sickness Virus on Flinders Technology Associates Card by Conventional Polymerase Chain Reaction. Animals (Basel), 14(8). https://doi.org/10.3390/ani14081242

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 8

Researcher Affiliations

Taesuji, Machimaporn
  • Clinic for Horse, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok 10530, Thailand.
Rattanamas, Khate
  • Master of Science Program in Animal Biotechnology, Mahanakorn University of Technology, Bangkok 10530, Thailand.
Yim, Peter B
  • Master of Science Program in Animal Biotechnology, Mahanakorn University of Technology, Bangkok 10530, Thailand.
Ruenphet, Sakchai
  • Master of Science Program in Animal Biotechnology, Mahanakorn University of Technology, Bangkok 10530, Thailand.
  • Immunology and Virology Department, Mahanakorn University of Technology, Bangkok 10530, Thailand.

Grant Funding

  • 64-RIO-4.1 / Mahanakorn University of Technology

Conflict of Interest Statement

The authors declare no conflict of interest.

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