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The American journal of pathology1985; 121(2); 361-368;

Stimulation of equine eosinophil migration by hydroxyacid metabolites of arachidonic acid.

Abstract: Lipoxygenase products of arachidonic acid are important mediators of inflammation, affecting several aspects of cell function. Monohydroxyeicosatetraenoic acid (mono-HETE) and 5,12-dihydroxyeicosatetraenoic acid (LTB4) enhance migration of both neutrophils and eosinophils in several species. The relative ability of positional isomers of HETE and of LTB4 to affect migration of equine eosinophils was studied. The 5, 8, 9, 11, 12, and 15 isomers of HETE were prepared by autooxidation of arachidonic acid, separated by sequential normal phase and reverse phase high performance liquid chromatography, and their identities verified by gas chromatography-mass spectrometry. Equine eosinophils were isolated to 30-70% purity on discontinuous metrizamide gradients. All isomers of HETE stimulated directed migration (chemotaxis) at concentrations ranging from 10(-5) to 10(-8) M. The relative activities of isomers were 11 greater than 9 = 8 = 5 greater than 12 greater than 15. The dihydroxy acid LTB4 maximally stimulated chemotaxis of equine eosinophils at a concentration of 3 X 10(-7) M. The eosinophil migration that resulted was less than the maximal stimulation observed in response to isomers of HETE. The results of our study suggest that equine eosinophils are excellent indicator cells for assay of arachidonic acid metabolites with chemotactic activity. Equine eosinophils are more sensitive to chemotactic stimulation by HETEs than cells from other animal species but are far less sensitive to stimulation by LTB4.
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Publication Date: 1985-11-01 PubMed ID: 4061569PubMed Central: PMC1888053
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  • Journal Article
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  • U.S. Gov't
  • P.H.S.

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study investigates the effect of specific metabolites of arachidonic acid in promoting the migration of eosinophils, a type of white blood cell, in horses. The specific metabolites studied are positional isomers of HETE and LTB4.

Methods

  • In order to perform the study, the researchers created 5, 8, 9, 11, 12, and 15 isomers of HETE by inducing autoxidation of arachidonic acid.
  • The identities of these isomers were confirmed using gas chromatography-mass spectrometry.
  • Eosinophils from horses were isolated to a purity level of 30-70% using discontinuous metrizamide gradients.

Findings

  • The researchers found that all isomers of HETE stimulated directed migration (chemotaxis) at concentrations ranging from 10(-5) to 10(-8) M.
  • The study further shows the relative activities of the isomers were 11 greater than 9 = 8 = 5 greater than 12 greater than 15.
  • The dihydroxy acid LTB4 maximally stimulated chemotaxis of equine eosinophils at a concentration of 3 X 10(-7) M.
  • The eosinophil migration that resulted was less than the maximal stimulation observed in response to isomers of HETE.

Conclusion

  • The findings of the study suggest that equine eosinophils are excellent indicator cells for assay of arachidonic acid metabolites with chemotactic activity.
  • Equine eosinophils are more sensitive to chemotactic stimulation by HETEs than cells from other animal species but are far less sensitive to stimulation by LTB4.

The study therefore contributes to our understanding of how inflammation is mediated in horses, which could have implications for addressing inflammatory conditions. The findings may also have broader relevance for understanding inflammation in other species.

Cite This Article

APA
Potter KA, Leid RW, Kolattukudy PE, Espelie KE. (1985). Stimulation of equine eosinophil migration by hydroxyacid metabolites of arachidonic acid. Am J Pathol, 121(2), 361-368.

Publication

The American journal of pathology
ISSN: 0002-9440
NlmUniqueID: 0370502
Country: United States
Language: English
Volume: 121
Issue: 2
Pages: 361-368

Researcher Affiliations

Potter, K A
    Leid, R W
      Kolattukudy, P E
        Espelie, K E

          MeSH Terms

          • Animals
          • Chemotaxis, Leukocyte
          • Chromatography, High Pressure Liquid
          • Eosinophils / immunology
          • Horses
          • Hydroxyeicosatetraenoic Acids / pharmacology

          Grant Funding

          • AI-07025 / NIAID NIH HHS
          • AI-17913 / NIAID NIH HHS
          • GM-07853 / NIGMS NIH HHS

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          Citations

          This article has been cited 3 times.
          1. Laegreid WW, Taylor SM, Silflow RM, Straub KM, Breeze RG, Liggitt HD, Leid RW. Production of lipoxygenase metabolites of eicosapentaenoic acid by bovine alveolar macrophages in vitro. Inflammation 1988 Oct;12(5):503-14.
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          2. McEwen BJ, Wilcock BP, Eyre P. The effect of leukotriene B4, leukotriene C4, zymosan activated serum, histamine, tabanid extract and N-formyl-methionyl-leucyl-phenylalanine on the in vitro migration of equine eosinophils. Can J Vet Res 1990 Oct;54(4):400-4.
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