Stress, Metabolic and Serum Muscle-Derived Enzymes Response of Horses Employed in Wooded Area and Field Trekking Courses.

This research investigates the impact of trekking, a non-competitive sports activity, on the metabolic responses, stress levels, and muscle damage markers of horses. The study specifically measures metabolic and muscle-related enzymes in blood before and after the activity, finding that extended trekking, particularly without adequate recovery periods, can incite metabolic changes and muscle damage in the equines.

Study Design and Methodology

• The research involved 30 horses, all of which were in good health. They were divided into three groups (A, B and C) based on different course lengths and schedules:
• Group A undertook a 15km trekking course.
• Group B engaged in a 35km trekking course.
• Group C followed a three-day schedule packed with three trekking courses, each trek covering 25km on the first and third days and 40km on the second day.

Blood Sampling and Analysis

• Blood samples were collected from each horse at different intervals. These included before the trek (TPRE), 5 minutes after the trek (TPOST), and 1 hour following the trek’s completion.
• The assessed values in the blood samples included lactate, glucose, total proteins, non-esterified fatty acids (NEFAs), creatinine, aspartate aminotransferase (AST), creatine phosphokinase (CPK), lactate dehydrogenase (LDH), and cortisol concentration.

Results and Findings

• The results revealed a significant increase in cortisol, lactate, glucose, NEFAs, creatinine, CPK, LDH and AST values following the trek compared to their resting values.
• It was observed that horses in Group C, participating in consecutive-day trekking courses, showed higher levels of the studied haematochemical parameters and muscle enzymes than the other groups (A and B).

Implications of the Study

• The findings of the study suggest that trekking leads to metabolic changes and sparks an acute stress response in horses.
• It emphasizes that this form of recreational exercise, if not coupled with sufficient recovery periods, may enhance the likelihood of muscle damage in these animals.
• Overall, the research indicates the need to consider the physiological impacts of trekking on horses and to ensure adequate resting periods to prevent potential harm.