Structure of the alpha 1 subunit of horse Na,K-ATPase gene.
Abstract: Genomic DNA for Na,K-ATPase alpha 1 subunit was obtained from libraries of horse kidney genomic DNA in Charon 4A and in EMBL3 bacteriophages by screening with the full sized cDNA probe of the alpha 1 subunit of rat Na,K-ATPase as probe. The gene spans 30 kb and consists of 23 exons and 22 intervening sequences. Intron-exon boundaries were analyzed. The protein-coding nucleotide sequence encodes 1016 amino acids with an Mr of 112,264. The putative amino acid sequence of horse alpha 1 is 96-97% homologous to those of other mammalian species.
Publication Date: 1989-06-19 PubMed ID: 2544461DOI: 10.1016/0014-5793(89)80691-xGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
Summary
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The research article details the structure of the Na,K-ATPase alpha 1 subunit gene in horses, obtained from horse kidney genomic DNA libraries. This gene spans 30 kb with 23 exons and 22 intervening sequences and encodes 1016 amino acids for protein production. The protein shows 96-97% similarity to those of other mammalian species.
Extraction of Genomic DNA
- The team obtained genomic DNA representing the Na,K-ATPase alpha 1 subunit from horse kidney libraries.
- These libraries were housed in Charon 4A and EMBL3 bacteriophages, effectively biological storage houses for DNA.
Screening and Identifying the Gene
- To locate the desired gene, scientists screened these libraries using a probe. This probe consisted of the full-sized complementary DNA (cDNA) of the alpha 1 subunit of rat Na,K-ATPase.
- Having identified the gene, the team discovered it spans over approximately 30 kilobases (kb) of DNA.
- This gene is not a continuous block but consists of 23 exons, or coding regions, and the 22 introns or ‘intervening sequences’ neglected during the transfer of genetic info from DNA to RNA.
Sequence Analysis and Protein Coding
- The researchers analyzed the boundaries between the exons and introns in the horse alpha 1 subunit gene, crucial to appreciate the gene’s structure.
- If transcribed and translated into a protein, the nucleotide sequence of this gene would code for 1016 amino acids. This is consistent with the typical size for this type of protein.
Comparative Homology
- The team compared the putative (presumed) amino acid sequence of the horse alpha 1 subunit with those of other mammalian species.
- The results showed an impressive 96-97% homology, suggesting the sequence and structure of this protein are highly conserved across different species of mammals.
Cite This Article
APA
Kano I, Nagai F, Satoh K, Ushiyama K, Nakao T, Kano K.
(1989).
Structure of the alpha 1 subunit of horse Na,K-ATPase gene.
FEBS Lett, 250(1), 91-98.
https://doi.org/10.1016/0014-5793(89)80691-x Publication
Researcher Affiliations
- Department of Toxicology, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- Exons
- Genes
- Horses
- Humans
- Kidney Medulla / enzymology
- Macromolecular Substances
- Molecular Sequence Data
- Restriction Mapping
- Sequence Homology, Nucleic Acid
- Sodium-Potassium-Exchanging ATPase / genetics
- Species Specificity
Citations
This article has been cited 10 times.- Johar K, Priya A, Wong-Riley MT. Regulation of Na(+)/K(+)-ATPase by nuclear respiratory factor 1: implication in the tight coupling of neuronal activity, energy generation, and energy consumption. J Biol Chem 2012 Nov 23;287(48):40381-90.
- Ko YH, Pedersen PL. Cystic fibrosis: a brief look at some highlights of a decade of research focused on elucidating and correcting the molecular basis of the disease. J Bioenerg Biomembr 2001 Dec;33(6):513-21.
- García-Sáez A, Perona R, Sastre L. Polymorphism and structure of the gene coding for the alpha 1 subunit of the Artemia franciscana Na/K-ATPase. Biochem J 1997 Jan 15;321 ( Pt 2)(Pt 2):509-18.
- Pathak BG, Neumann JC, Croyle ML, Lingrel JB. The presence of both negative and positive elements in the 5'-flanking sequence of the rat Na,K-ATPase alpha 3 subunit gene are required for brain expression in transgenic mice. Nucleic Acids Res 1994 Nov 11;22(22):4748-55.
- Shamraj OI, Lingrel JB. A putative fourth Na+,K(+)-ATPase alpha-subunit gene is expressed in testis. Proc Natl Acad Sci U S A 1994 Dec 20;91(26):12952-6.
- Kobayashi M, Kawakami K. ATF-1CREB heterodimer is involved in constitutive expression of the housekeeping Na,K-ATPase alpha 1 subunit gene. Nucleic Acids Res 1995 Aug 11;23(15):2848-55.
- . New nucleotide sequence data on the EMBL File Server. Nucleic Acids Res 1989 Nov 25;17(22):9511-24.
- Sverdlov ED. The genes of Na,K-ATPase, a selfreview. Genetica 1991;85(1):91-101.
- Wimmers LE, Ewing NN, Bennett AB. Higher plant Ca(2+)-ATPase: primary structure and regulation of mRNA abundance by salt. Proc Natl Acad Sci U S A 1992 Oct 1;89(19):9205-9.
- Suzuki-Yagawa Y, Kawakami K, Nagano K. Housekeeping Na,K-ATPase alpha 1 subunit gene promoter is composed of multiple cis elements to which common and cell type-specific factors bind. Mol Cell Biol 1992 Sep;12(9):4046-55.
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