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Home / Equine Research Bank / Vet Sci / Study on NGF and VEGF during the Equine Perinatal Period-Par...
Veterinary sciences2022; 9(9); 451; doi: 10.3390/vetsci9090451

Study on NGF and VEGF during the Equine Perinatal Period-Part 1: Healthy Foals Born from Normal Pregnancy and Parturition.

Abstract: The importance of trophic factors, such as nerve growth factor (NGF), vascular endothelial growth factor (VEGF), and brain-derived neurotrophic factor (BDNF) during the perinatal period, is now emerging. Through their functional activities of neurogenesis and angiogenesis, they play a key role in the final maturation of the nervous and vascular systems. The present study aims to: (i) evaluate the NGF and VEGF levels obtained at parturition from the mare, foal and umbilical cord vein plasma, as well as in amniotic fluid; (ii) evaluate NGF and VEGF content in the plasma of healthy foals during the first 72 h of life (T0, T24 and T72); (iii) evaluate NGF and VEGF levels at parturition in relation to the selected mares’ and foals’ clinical parameters; (iv) evaluate the relationship between the two trophic factors and the thyroid hormone levels (TT3 and TT4) in the first 72 h of life; (v) assess mRNA expression of NGF, VEGF and BDNF and their cell surface receptors in the placenta. Fourteen Standardbred healthy foals born from mares with normal pregnancies and parturitions were included in the study. The dosage of NGF and VEGF levels was performed using commercial ELISA kits, whereas NGF, VEGF and BDNF placental gene expression was performed using semi-quantitative real-time PCR. In foal plasma, both NGF and VEGF levels decreased significantly over time, from T0 to T24 (p = 0.0066 for NGF; p < 0.0001 for VEGF) and from T0 to T72 (p = 0.0179 for NGF; p = 0.0016 for VEGF). In foal serum, TT3 levels increased significantly over time from T0 to T24 (p = 0.0058) and from T0 to T72 (p = 0.0013), whereas TT4 levels decreased significantly over time from T0 to T24 (p = 0.0201) and from T0 to T72 (p < 0.0001). A positive correlation was found in the levels of NGF and VEGF in foal plasma at each time point (p = 0.0115; r = 0.2862). A positive correlation was found between NGF levels in the foal plasma at T0 and lactate (p = 0.0359; r = 0.5634) as well as between VEGF levels in the foal plasma at T0 and creatine kinase (p = 0.0459; r = 0.5407). VEGF was expressed in all fetal membranes, whereas NGF and its receptors were not expressed in the amnion. The close relationship between the two trophic factors in foal plasma over time and their fine expression in placental tissues appear to be key regulators of fetal development and adaptation to extra-uterine life.
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Publication Date: 2022-08-23 PubMed ID: 36136667PubMed Central: PMC9504588DOI: 10.3390/vetsci9090451Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on assessing the presence and role of trophic factors (specifically nerve growth factor (NGF) and vascular endothelial growth factor (VEGF)) in foals during the perinatal period. It highlights their correlation with developmental processes and adaptation to extra-uterine life.

Objective and Methodology of the Study

  • The study aims to quantify the levels of trophic factors NGF and VEGF at parturition in the mare, foal, umbilical cord vein plasma, amniotic fluid, and evaluate their progression in foal plasma during the first 72 hours of life.
  • It also seeks to understand the correlation of these growth factors with selected clinical parameters and thyroid hormone levels in mares and foals during parturition.
  • The expression of NGF, VEGF, and another factor, brain-derived neurotropic factor (BDNF), and their cell surface receptors in the placenta was assessed.
  • The subjects of this study were 14 healthy foals born from mares with normal pregnancies and parturitions.
  • Commercial ELISA kits were used to measure NGF and VEGF levels, while semi-quantitative real-time PCR was used to assay the gene expression in the placenta.

Key Findings of the Study

  • The study found that both NGF and VEGF levels in the foal’s plasma descended significantly in the first 72 hours after birth.
  • Contrarily, the TT3 hormone levels elevated while TT4 levels fell in foal serum during the same timeframe.
  • The study observed a positive correlation between NGF and VEGF levels in the foal plasma.
  • Additionally, there were positive correlations between NGF levels in foal plasma and lactate and between VEGF levels in the foal plasma and creatine kinase.
  • VEGF was found to be expressed in all examined fetal membranes, while NGF and its receptors had no expression in the amnion.
  • In summary, a tight relationship between NGF and VEGF in foal plasma over time and their distinct expression in placental tissues appear crucial to the developmental processes and adaptation to extra-uterine life.

Conclusion

  • The study offers valuable insights into the roles of trophic factors like NGF and VEGF in the development and adaptation stages of a foal’s life during the perinatal period.
  • Understanding these mechanisms may have important implications for maintaining the health and vitality of newborn foals and further contribute to advancing veterinary medicine and equine neonatal care.

Cite This Article

APA
Ellero N, Lanci A, Baldassarro VA, Alastra G, Mariella J, Cescatti M, Giardino L, Castagnetti C. (2022). Study on NGF and VEGF during the Equine Perinatal Period-Part 1: Healthy Foals Born from Normal Pregnancy and Parturition. Vet Sci, 9(9), 451. https://doi.org/10.3390/vetsci9090451

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 9
Issue: 9
PII: 451

Researcher Affiliations

Ellero, Nicola
  • Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, 40064 Bologna, Italy.
Lanci, Aliai
  • Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, 40064 Bologna, Italy.
Baldassarro, Vito Antonio
  • Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, 40064 Bologna, Italy.
Alastra, Giuseppe
  • Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, 40064 Bologna, Italy.
Mariella, Jole
  • Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, 40064 Bologna, Italy.
Cescatti, Maura
  • IRET Foundation, 40064 Bologna, Italy.
Giardino, Luciana
  • Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, 40064 Bologna, Italy.
  • Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, 40064 Bologna, Italy.
Castagnetti, Carolina
  • Department of Veterinary Medical Sciences (DIMEVET), University of Bologna, 40064 Bologna, Italy.
  • Health Science and Technologies Interdepartmental Center for Industrial Research (HST-ICIR), University of Bologna, 40064 Bologna, Italy.

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 57 references
  1. Tucker KL, Meyer M, Barde YA. Neurotrophins are required for nerve growth during development.. Nat Neurosci 2001 Jan;4(1):29-37.
    doi: 10.1038/82868pubmed: 11135642google scholar: lookup
  2. Chao MV. Trophic factors: An evolutionary cul-de-sac or door into higher neuronal function?. J Neurosci Res 2000 Feb 1;59(3):353-5.
    doi: 10.1002/(SICI)1097-4547(20000201)59:3<353::AID-JNR8>3.0.CO;2-Spubmed: 10679770google scholar: lookup
  3. Lu B, Figurov A. Role of neurotrophins in synapse development and plasticity.. Rev Neurosci 1997 Jan-Mar;8(1):1-12.
    doi: 10.1515/REVNEURO.1997.8.1.1pubmed: 9402641google scholar: lookup
  4. Takei N, Nawa H. Roles of neurotrophins on synaptic development and functions in the central nervous system.. Hum Cell 1998 Sep;11(3):157-65.
    pubmed: 10086277
  5. Frade JM, Rodríguez-Tébar A, Barde YA. Induction of cell death by endogenous nerve growth factor through its p75 receptor.. Nature 1996 Sep 12;383(6596):166-8.
    doi: 10.1038/383166a0pubmed: 8774880google scholar: lookup
  6. Stadelmann C, Kerschensteiner M, Misgeld T, Brück W, Hohlfeld R, Lassmann H. BDNF and gp145trkB in multiple sclerosis brain lesions: neuroprotective interactions between immune and neuronal cells?. Brain 2002 Jan;125(Pt 1):75-85.
    doi: 10.1093/brain/awf015pubmed: 11834594google scholar: lookup
  7. Skaper SD. Nerve growth factor: a neuroimmune crosstalk mediator for all seasons.. Immunology 2017 May;151(1):1-15.
    doi: 10.1111/imm.12717pmc: PMC5382350pubmed: 28112808google scholar: lookup
  8. Calzà L, Giardino L, Aloe L. NGF content and expression in the rat pituitary gland and regulation by thyroid hormone.. Brain Res Mol Brain Res 1997 Nov;51(1-2):60-8.
    doi: 10.1016/S0169-328X(97)00213-1pubmed: 9427507google scholar: lookup
  9. Gostynska N, Pannella M, Rocco ML, Giardino L, Aloe L, Calzà L. The pleiotropic molecule NGF regulates the in vitro properties of fibroblasts, keratinocytes, and endothelial cells: implications for wound healing.. Am J Physiol Cell Physiol 2020 Feb 1;318(2):C360-C371.
    doi: 10.1152/ajpcell.00180.2019pubmed: 31774700google scholar: lookup
  10. Pius-Sadowska E, Machaliński B. Pleiotropic activity of nerve growth factor in regulating cardiac functions and counteracting pathogenesis.. ESC Heart Fail 2021 Apr;8(2):974-987.
    doi: 10.1002/ehf2.13138pmc: PMC8006610pubmed: 33465292google scholar: lookup
  11. Amagai Y, Sato H, Ishizaka S, Matsuda K, Aurich C, Tanaka A, Matsuda H. Cloning and Expression of Equine β-Nerve Growth Factor. J. Equine Vet. Sci. 2016;45:28–31.
    doi: 10.1016/j.jevs.2016.06.001google scholar: lookup
  12. Charnock-Jones DS, Kaufmann P, Mayhew TM. Aspects of human fetoplacental vasculogenesis and angiogenesis. I. Molecular regulation.. Placenta 2004 Feb-Mar;25(2-3):103-13.
    doi: 10.1016/j.placenta.2003.10.004pubmed: 14972443google scholar: lookup
  13. Allen WR, Gower S, Wilsher S. Immunohistochemical localization of vascular endothelial growth factor (VEGF) and its two receptors (Flt-I and KDR) in the endometrium and placenta of the mare during the oestrous cycle and pregnancy.. Reprod Domest Anim 2007 Oct;42(5):516-26.
    doi: 10.1111/j.1439-0531.2006.00815.xpubmed: 17845608google scholar: lookup
  14. Li Y, Zhang F, Nagai N, Tang Z, Zhang S, Scotney P, Lennartsson J, Zhu C, Qu Y, Fang C, Hua J, Matsuo O, Fong GH, Ding H, Cao Y, Becker KG, Nash A, Heldin CH, Li X. VEGF-B inhibits apoptosis via VEGFR-1-mediated suppression of the expression of BH3-only protein genes in mice and rats.. J Clin Invest 2008 Mar;118(3):913-23.
    doi: 10.1172/JCI33673pmc: PMC2230661pubmed: 18259607google scholar: lookup
  15. Jin K, Zhu Y, Sun Y, Mao XO, Xie L, Greenberg DA. Vascular endothelial growth factor (VEGF) stimulates neurogenesis in vitro and in vivo.. Proc Natl Acad Sci U S A 2002 Sep 3;99(18):11946-50.
    doi: 10.1073/pnas.182296499pmc: PMC129374pubmed: 12181492google scholar: lookup
  16. Sawicka-Gutaj N, Zawalna N, Gut P, Ruchała M. Relationship between thyroid hormones and central nervous system metabolism in physiological and pathological conditions.. Pharmacol Rep 2022 Oct;74(5):847-858.
    doi: 10.1007/s43440-022-00377-wpubmed: 35771431google scholar: lookup
  17. Calza L, Fernandez M, Giuliani A, Aloe L, Giardino L. Thyroid hormone activates oligodendrocyte precursors and increases a myelin-forming protein and NGF content in the spinal cord during experimental allergic encephalomyelitis.. Proc Natl Acad Sci U S A 2002 Mar 5;99(5):3258-63.
    doi: 10.1073/pnas.052704499pmc: PMC122506pubmed: 11867745google scholar: lookup
  18. Santschi E.M., Vaala W.E.. Identification of the high-risk pregnancy. In: McKinnon A.O., Squires E.L., Vaala W.E., Varner D.D., editors. Equine Reproduction. Wiley-Blackwell; Oxford, UK: 2011. pp. 5–15.
  19. Lanci A, Mariella J, Ellero N, Faoro A, Peric T, Prandi A, Freccero F, Castagnetti C. Hair Cortisol and DHEA-S in Foals and Mares as a Retrospective Picture of Feto-Maternal Relationship under Physiological and Pathological Conditions.. Animals (Basel) 2022 May 14;12(10).
    doi: 10.3390/ani12101266pmc: PMC9138058pubmed: 35625111google scholar: lookup
  20. Frazer G.S., Perkins N.R., Embertson R.M.. Normal parturition and evaluation of the mare in dystocia. Equine Vet. Educ. 1999;11:41–46.
    doi: 10.1111/j.2042-3292.1999.tb00918.xgoogle scholar: lookup
  21. Vaala WE, Sertich PL. Management strategies for mares at risk for periparturient complications.. Vet Clin North Am Equine Pract 1994 Apr;10(1):237-65.
    doi: 10.1016/S0749-0739(17)30376-0pubmed: 8039034google scholar: lookup
  22. Ellero N, Lanci A, Ferlizza E, Andreani G, Mariella J, Isani G, Castagnetti C. Activities of matrix metalloproteinase-2 and -9 in amniotic fluid at parturition in mares with normal and high-risk pregnancy.. Theriogenology 2021 Sep 15;172:116-122.
    doi: 10.1016/j.theriogenology.2021.06.009pubmed: 34153567google scholar: lookup
  23. Vaala W.E., House J.K., Madigan J.E.. Initial management and physical examination of the neonate. Mosby; St. Louis, MO, USA: 2002. pp. 277–293.
  24. Bianco C, Pirrone A, Boldini S, Sarli G, Castagnetti C. Histomorphometric parameters and fractal complexity of the equine placenta from healthy and sick foals.. Theriogenology 2014 Nov;82(8):1106-12.
    doi: 10.1016/j.theriogenology.2014.07.036pubmed: 25193631google scholar: lookup
  25. Lopez-Rodriguez MF, Cymbaluk NF, Epp T, Laarveld B, Thrasher M, Card CE. A Field Study of Serum, Colostrum, Milk Iodine, and Thyroid Hormone Concentrations in Postpartum Draft Mares and Foals.. J Equine Vet Sci 2020 Jul;90:103018.
    doi: 10.1016/j.jevs.2020.103018pubmed: 32534782google scholar: lookup
  26. Lopez-Rodriguez MF, Cymbaluk N, Epp T, Laarveld B, Serrano Recalde EC, Simko E, Card C. Effects of the Glucosinolate Sinigrin in Combination With a Noniodine Supplemented Diet on Serum Iodine and Thyroid Hormone Concentrations in Nonpregnant Mares.. J Equine Vet Sci 2020 Aug;91:103110.
    doi: 10.1016/j.jevs.2020.103110pubmed: 32684255google scholar: lookup
  27. Harvey J.W.. Normal hematologic values. In: Koterba A.M., Drummond W.H., Kosch P.C., editors. Equine Clinical Neonatology. Lea and Febiger; Philadelphia, PA, USA: 1990. pp. 561–570.
  28. Bauer JE, Harvey JW, Asquith RL, McNulty PK, Kivipelto J. Clinical chemistry reference values of foals during the first year of life.. Equine Vet J 1984 Jul;16(4):361-3.
    doi: 10.1111/j.2042-3306.1984.tb01944.xpubmed: 6479134google scholar: lookup
  29. Stoneham SJ, Palmer L, Cash R, Rossdale PD. Measurement of serum amyloid A in the neonatal foal using a latex agglutination immunoturbidimetric assay: determination of the normal range, variation with age and response to disease.. Equine Vet J 2001 Nov;33(6):599-603.
    doi: 10.2746/042516401776563472pubmed: 11720032google scholar: lookup
  30. Pirrone A, Mariella J, Gentilini F, Castagnetti C. Amniotic fluid and blood lactate concentrations in mares and foals in the early postpartum period.. Theriogenology 2012 Oct 1;78(6):1182-9.
    doi: 10.1016/j.theriogenology.2012.02.032pubmed: 22898010google scholar: lookup
  31. Malamitsi-Puchner A, Nikolaou KE, Economou E, Boutsikou M, Boutsikou T, Kyriakakou M, Puchner KP, Hassiakos D. Intrauterine growth restriction and circulating neurotrophin levels at term.. Early Hum Dev 2007 Jul;83(7):465-9.
    doi: 10.1016/j.earlhumdev.2006.09.001pubmed: 17071024google scholar: lookup
  32. Hogan KA, Ambler CA, Chapman DL, Bautch VL. The neural tube patterns vessels developmentally using the VEGF signaling pathway.. Development 2004 Apr;131(7):1503-13.
    doi: 10.1242/dev.01039pubmed: 14998923google scholar: lookup
  33. Schwarz Q, Gu C, Fujisawa H, Sabelko K, Gertsenstein M, Nagy A, Taniguchi M, Kolodkin AL, Ginty DD, Shima DT, Ruhrberg C. Vascular endothelial growth factor controls neuronal migration and cooperates with Sema3A to pattern distinct compartments of the facial nerve.. Genes Dev 2004 Nov 15;18(22):2822-34.
    doi: 10.1101/gad.322904pmc: PMC528901pubmed: 15545635google scholar: lookup
  34. Aisa MC, Barbati A, Cappuccini B, De Rosa F, Gerli S, Clerici G, Kaptilnyy VA, Ishenko AI, Di Renzo GC. Urinary Nerve Growth Factor in full-term, preterm and intra uterine growth restriction neonates: Association with brain growth at 30-40 days of postnatal period and with neuro-development outcome at two years. A pilot study.. Neurosci Lett 2021 Jan 10;741:135459.
    doi: 10.1016/j.neulet.2020.135459pubmed: 33223047google scholar: lookup
  35. Williams MA, Schmidt AR, Carleton CL, Darien BJ, Goyert GL, Sokol RJ, Derksen FJ. Amniotic fluid analysis for ante-partum foetal assessment in the horse.. Equine Vet J 1992 May;24(3):236-8.
    doi: 10.1111/j.2042-3306.1992.tb02821.xpubmed: 1606938google scholar: lookup
  36. Kodomari I, Wada E, Nakamura S, Wada K. Maternal supply of BDNF to mouse fetal brain through the placenta.. Neurochem Int 2009 Feb;54(2):95-8.
    doi: 10.1016/j.neuint.2008.11.005pubmed: 19114070google scholar: lookup
  37. Marx CE, Vance BJ, Jarskog LF, Chescheir NC, Gilmore JH. Nerve growth factor, brain-derived neurotrophic factor, and neurotrophin-3 levels in human amniotic fluid.. Am J Obstet Gynecol 1999 Nov;181(5 Pt 1):1225-30.
    doi: 10.1016/S0002-9378(99)70113-4pubmed: 10561650google scholar: lookup
  38. Dhobale M, Mehendale S, Pisal H, Nimbargi V, Joshi S. Reduced maternal and cord nerve growth factor levels in preterm deliveries.. Int J Dev Neurosci 2012 Apr;30(2):99-103.
    doi: 10.1016/j.ijdevneu.2011.12.007pubmed: 22245319google scholar: lookup
  39. Sabour S. Prediction of preterm delivery using levels of VEGF and leptin in amniotic fluid from the second trimester: prediction rules.. Arch Gynecol Obstet 2015 Apr;291(4):719.
    doi: 10.1007/s00404-014-3568-ypubmed: 25490880google scholar: lookup
  40. De Backer D. Lactic acidosis.. Intensive Care Med 2003 May;29(5):699-702.
    doi: 10.1007/s00134-003-1746-7pubmed: 12682722google scholar: lookup
  41. Chiba A., Aoki T., Itoh M., Yamagishi N., Shibano K.. Hematological and Blood Biochemical Characteristics of Newborn Heavy Draft Foals After Dystocia. J. Equine Vet. Sci. 2017;50:69–75.
    doi: 10.1016/j.jevs.2016.10.013google scholar: lookup
  42. Pirrone A, Panzani S, Govoni N, Castagnetti C, Veronesi MC. Thyroid hormone concentrations in foals affected by perinatal asphyxia syndrome.. Theriogenology 2013 Oct 1;80(6):624-9.
    doi: 10.1016/j.theriogenology.2013.06.003pubmed: 23849257google scholar: lookup
  43. Walker P, Weil ML, Weichsel ME Jr, Fisher DA. Effect of thyroxine on nerve growth factor concentration in neonatal mouse brain.. Life Sci 1981 Apr 13-20;28(15-16):1777-87.
    doi: 10.1016/0024-3205(81)90349-0pubmed: 7242260google scholar: lookup
  44. Figueiredo BC, Almazan G, Ma Y, Tetzlaff W, Miller FD, Cuello AC. Gene expression in the developing cerebellum during perinatal hypo- and hyperthyroidism.. Brain Res Mol Brain Res 1993 Mar;17(3-4):258-68.
    doi: 10.1016/0169-328X(93)90010-Mpubmed: 7685463google scholar: lookup
  45. Lanci A, Ingrà L, Dondi F, Tomasello F, Teti G, Mariella J, Falconi M, Castagnetti C. Morphological study of equine amniotic compartment.. Theriogenology 2022 Jan 1;177:165-171.
    doi: 10.1016/j.theriogenology.2021.10.019pubmed: 34710648google scholar: lookup
  46. Fujita K, Tatsumi K, Kondoh E, Chigusa Y, Mogami H, Fujii T, Yura S, Kakui K, Konishi I. Differential expression and the anti-apoptotic effect of human placental neurotrophins and their receptors.. Placenta 2011 Oct;32(10):737-44.
    doi: 10.1016/j.placenta.2011.07.001pubmed: 21831423google scholar: lookup
  47. Toti P, Ciarmela P, Florio P, Volpi N, Occhini R, Petraglia F. Human placenta and fetal membranes express nerve growth factor mRNA and protein.. J Endocrinol Invest 2006 Apr;29(4):337-41.
    doi: 10.1007/BF03344105pubmed: 16699300google scholar: lookup
  48. Frank P, Barrientos G, Tirado-González I, Cohen M, Moschansky P, Peters EM, Klapp BF, Rose M, Tometten M, Blois SM. Balanced levels of nerve growth factor are required for normal pregnancy progression.. Reproduction 2014 Aug;148(2):179-89.
    doi: 10.1530/REP-14-0112pubmed: 24825909google scholar: lookup
  49. Zacchigna S, Lambrechts D, Carmeliet P. Neurovascular signalling defects in neurodegeneration.. Nat Rev Neurosci 2008 Mar;9(3):169-81.
    doi: 10.1038/nrn2336pubmed: 18253131google scholar: lookup
  50. Wessels JM, Wu L, Leyland NA, Wang H, Foster WG. The brain-uterus connection: brain derived neurotrophic factor (BDNF) and its receptor (Ntrk2) are conserved in the mammalian uterus.. PLoS One 2014;9(4):e94036.
    pmc: PMC3979719pubmed: 24714156doi: 10.1371/journal.pone.0094036google scholar: lookup
  51. Cassens C, Kleene R, Xiao MF, Friedrich C, Dityateva G, Schafer-Nielsen C, Schachner M. Binding of the receptor tyrosine kinase TrkB to the neural cell adhesion molecule (NCAM) regulates phosphorylation of NCAM and NCAM-dependent neurite outgrowth.. J Biol Chem 2010 Sep 10;285(37):28959-67.
    doi: 10.1074/jbc.M110.114835pmc: PMC2937923pubmed: 20605779google scholar: lookup
  52. Nakamura K, Martin KC, Jackson JK, Beppu K, Woo CW, Thiele CJ. Brain-derived neurotrophic factor activation of TrkB induces vascular endothelial growth factor expression via hypoxia-inducible factor-1alpha in neuroblastoma cells.. Cancer Res 2006 Apr 15;66(8):4249-55.
    doi: 10.1158/0008-5472.CAN-05-2789pubmed: 16618748google scholar: lookup
  53. Wang LH, Paden AJ, Johnson EM Jr. Mixed-lineage kinase inhibitors require the activation of Trk receptors to maintain long-term neuronal trophism and survival.. J Pharmacol Exp Ther 2005 Mar;312(3):1007-19.
    doi: 10.1124/jpet.104.077800pubmed: 15525794google scholar: lookup
  54. Tervonen TA, Ajamian F, De Wit J, Verhaagen J, Castrén E, Castrén M. Overexpression of a truncated TrkB isoform increases the proliferation of neural progenitors.. Eur J Neurosci 2006 Sep;24(5):1277-85.
    doi: 10.1111/j.1460-9568.2006.05010.xpubmed: 16987215google scholar: lookup
  55. Garcés MF, Sanchez E, Torres-Sierra AL, Ruíz-Parra AI, Angel-Müller E, Alzate JP, Sánchez ÁY, Gomez MA, Romero XC, Castañeda ZE, Sanchez-Rebordelo E, Diéguez C, Nogueiras R, Caminos JE. Brain-derived neurotrophic factor is expressed in rat and human placenta and its serum levels are similarly regulated throughout pregnancy in both species.. Clin Endocrinol (Oxf) 2014 Jul;81(1):141-51.
    doi: 10.1111/cen.12391pubmed: 24372023google scholar: lookup
  56. Dini P, Carossino M, Loynachan AT, El-Sheikh Ali H, Wolfsdorf KE, Scoggin KE, Daels P, Ball BA. Equine hydrallantois is associated with impaired angiogenesis in the placenta.. Placenta 2020 Apr;93:101-112.
    doi: 10.1016/j.placenta.2020.03.001pubmed: 32250734google scholar: lookup
  57. Daneshmand SS, Cheung CY, Brace RA. Regulation of amniotic fluid volume by intramembranous absorption in sheep: role of passive permeability and vascular endothelial growth factor.. Am J Obstet Gynecol 2003 Mar;188(3):786-93.
    doi: 10.1067/mob.2003.160pubmed: 12634658google scholar: lookup

Citations

This article has been cited 1 times.
  1. Ellero N, Lanci A, Baldassarro VA, Alastra G, Mariella J, Cescatti M, Castagnetti C, Giardino L. Study on NGF and VEGF during the Equine Perinatal Period-Part 2: Foals Affected by Neonatal Encephalopathy.. Vet Sci 2022 Aug 26;9(9).
    doi: 10.3390/vetsci9090459pubmed: 36136675google scholar: lookup
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