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Reproduction in domestic animals = Zuchthygiene2006; 41(6); 535-537; doi: 10.1111/j.1439-0531.2006.00709.x

Successful timing of ovulation using deslorelin (Ovuplant) is labour-saving in mares aimed for single ai with frozen semen.

Abstract: To minimize the number of matings/inseminations, controlled ovulation has been practised since a long time ago. A potent short-term implant, releasing the GnRH analogue deslorelin (Ovuplant((R))) has been used in Australia and North America for several years for hastening the ovulation time in mares, but the product is not registered on the European market. This study was aimed to investigate: (1) ovulation time in mares implanted with Ovuplant when the largest follicle was 42 mm or more in size, (2) repeatability of ovulation time in successive oestruses when treated with Ovuplant, (3) pregnancy rate after single insemination with frozen-thawed semen near ovulation. This study included 11 mares, and altogether 17 timed ovulations. Follicular growth and ovulation were determined by palpation per rectum and by ultrasonography in the morning (at 7:00 hours) every second day until observation of a follicle of at least 42 mm in diameter. Then the mares were re-examined in the afternoon (at 19:00 hours), and an Ovuplant was inserted in the mucosa of the vulva. For detection of ovulation, the mares were palpated and ultrasounded repeatedly from 36-42 h after the insert. The mares were inseminated with frozen-thawed semen once at ovulation. All mares ovulated at 36-48 h after treatment and 94% at 38-42 h after treatment. The six mares that were treated at two oestruses ovulated at 39.9 and 39.7 h, respectively. Five of 11 mares (45.4%), inseminated with frozen-thawed semen at the first oestrous cycle were pregnant day 14-16 after ovulation. Using this protocol, there is no need of palpation/ultrasonography during night hours, and examination at 36 and 41 h after implantation might be enough for estimation of ovulation time.
Publication Date: 2006-11-17 PubMed ID: 17107513DOI: 10.1111/j.1439-0531.2006.00709.xGoogle Scholar: Lookup
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  • Journal Article

Summary

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The research article explores the use of deslorelin (Ovuplant) to successfully time ovulation in mares for single artificial insemination using frozen semen, making the process more efficient and increasing chances of pregnancy.

Research Aims and Methodology

  • The research had three primary objectives. The first to establish the ovulation time in mares implanted with deslorelin (Ovuplant) when the largest follicle was 42 mm or more. The second target was to assess the repeatability of ovulation time in successive estrus cycles when treated with deslorelin. Lastly, the paper sought to investigate the pregnancy rate after a single insemination with frozen-thawed semen.
  • The study included a total of 11 mares, with 17 timed ovulations in total. The growth of the follicular and ovulation was determined by rectal palpation and ultrasonography every two days. When a follicle of at least 42mm in diameter was observed, an Ovuplant was inserted into the vulva’s mucosa and the mares re-examined.
  • Signs of ovulation were tracked by repeated palpation and ultrasonography 36-42 hours after the implant insertion. The mares were then inseminated once at the moment of ovulation with the thawed frozen semen.

Findings and Conclusion

  • All of the mares in the study ovulated 36-48 hours post-treatment, with 94% ovulating 38-42 hours post treatment.
  • Mares treated in two estrus cycles ovulated around the same time (39.9 and 39.7 hours, respectively).
  • Nearly half (45.4%) of the mares inseminated with thawed frozen semen during the first estrus cycle successfully became pregnant between day 14-16 post ovulation.
  • The research demonstrated that there is no need for nighttime palpation/ultrasonography and examination at 36 and 41 hours post-implantation could be sufficient to estimate ovulation time.
  • Hence, the use of deslorelin (Ovuplant) to time ovulation in mares proves to be an efficient method in managing single artificial insemination using frozen semen. The process in turn could lower labour costs and increase the chances of successful pregnancies.

Cite This Article

APA
Hemberg E, Lundeheim N, Einarsson S. (2006). Successful timing of ovulation using deslorelin (Ovuplant) is labour-saving in mares aimed for single ai with frozen semen. Reprod Domest Anim, 41(6), 535-537. https://doi.org/10.1111/j.1439-0531.2006.00709.x

Publication

ISSN: 0936-6768
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 41
Issue: 6
Pages: 535-537

Researcher Affiliations

Hemberg, E
  • Herrgården, Haddebo Bruk, SE-697 93 Hjortkvarn, Sweden.
Lundeheim, N
    Einarsson, S

      MeSH Terms

      • Animals
      • Cryopreservation / veterinary
      • Digital Rectal Examination / veterinary
      • Enzyme Inhibitors
      • Female
      • Gonadotropin-Releasing Hormone / analogs & derivatives
      • Horses / physiology
      • Insemination, Artificial / veterinary
      • Ovulation / drug effects
      • Ovulation / physiology
      • Ovulation Detection / methods
      • Ovulation Detection / veterinary
      • Ovulation Induction / methods
      • Ovulation Induction / veterinary
      • Pregnancy
      • Pregnancy Outcome
      • Pregnancy Rate
      • Reproducibility of Results
      • Semen Preservation / methods
      • Semen Preservation / veterinary
      • Time Factors
      • Triptorelin Pamoate / analogs & derivatives
      • Triptorelin Pamoate / pharmacology

      Citations

      This article has been cited 3 times.
      1. Teng M, Zhao M, Mu B, Lei A. Allogenic Follicular Fosterage Technology: Problems, Progress and Potential. Vet Sci 2024 Jun 17;11(6).
        doi: 10.3390/vetsci11060276pubmed: 38922023google scholar: lookup
      2. Kowalczyk A, Czerniawska-Piątkowska E, Kuczaj M. Factors Influencing the Popularity of Artificial Insemination of Mares in Europe. Animals (Basel) 2019 Jul 19;9(7).
        doi: 10.3390/ani9070460pubmed: 31331026google scholar: lookup
      3. Martinez de Andino EV, Brom-de-Luna JG, Canesin HS, Rader K, Resende HL, Ripley AM, Love CC, Hinrichs K. Intrafollicular oocyte transfer in the horse: effect of autologous vs. allogeneic transfer and time of administration of ovulatory stimulus before transfer. J Assist Reprod Genet 2019 Jun;36(6):1237-1250.
        doi: 10.1007/s10815-019-01460-7pubmed: 31073725google scholar: lookup