Taxon-specific probes for the cellulolytic genus Fibrobacter reveal abundant and novel equine-associated populations.
Abstract: A total of six 16S rRNA targeted oligonucleotide probes were used to quantify Fibrobacter abundance and diversity in the gastrointestinal contents of a pony. Approximately 12% of the total 16S rRNA extracted from cecal contents hybridized with a Fibrobacter genus-specific probe and a Fibrobacter succinogenes species-specific probe. However, no significant hybridization was observed with a probe for the species. Fibrobacter intestinalis or with three probes for F. succinogenes subspecies. This suggested the presence of a previously undescribed population of F. succinogenes-like organisms. Novel lineages of F. succinogenes were subsequently identified by using PCR primers specific for the genus to amplify sequences coding for 16S rRNA from DNA extracted from cecal contents. Sequences of the cloned amplification products were shown to be affiliated with F. succinogenes but represented two distinct, and novel, lines of descent within the species.
Publication Date: 1995-04-01 PubMed ID: 7538274PubMed Central: PMC167390DOI: 10.1128/aem.61.4.1348-1351.1995Google Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- Non-P.H.S.
Summary
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The research focuses on the discovery and quantification of specific types of bacteria known as Fibrobacter, found within the gut of a pony. Interestingly, the analysis suggests that these bacteria could belong to variants of the species not previously described.
Overview of the Research
- The research article addresses the study of how the genus Fibrobacter, which are cellulose-digesting (cellulolytic) bacteria, is distributed and diversified in the gastrointestinal tract of a pony.
- The researchers used six 16S rRNA (ribosomal RNA) targeted oligonucleotide probes to investigate both the abundance and genetic diversity of Fibrobacter.
Key Research Findings
- About 12% of all the 16S rRNA extracted from the cecal contents of the pony hybridized with probes specific to the genus Fibrobacter and the species Fibrobacter succinogenes. The cecum is part of the large intestine, and in many animals, plays an important role in the digestion of plant material.
- No significant hybridization was observed with a probe for another species, Fibrobacter intestinalis, or with three probes for subspecies of F. succinogenes.
- This finding indicates that the bacterial population present in the pony’s gut might be a different type of F. succinogenes not identified before.
Novel Classifications within Fibrobacter
- The researchers then further explored the newly discovered population by using genus-specific primers to amplify the 16S rRNA sequences from the cecal contents.
- The sequences obtained were affiliated with F. succinogenes; however, they represented two distinct and novel lines of descent within the species. This suggests that there may be previously unidentified subspecies present in the equine gut.
Implications of the Research
- The discovery of novel Fibrobacter species or subspecies could have implications for understanding the microbiome of equine species and how these animals digest plant material.
- This research also emphasizes the importance of using rRNA-targeted probes in microbiological studies to reveal potentially novel microbial lineages, which might be overlooked using traditional culture-based methods.
Cite This Article
APA
Lin C, Stahl DA.
(1995).
Taxon-specific probes for the cellulolytic genus Fibrobacter reveal abundant and novel equine-associated populations.
Appl Environ Microbiol, 61(4), 1348-1351.
https://doi.org/10.1128/aem.61.4.1348-1351.1995 Publication
Researcher Affiliations
- Department of Veterinary Pathobiology, University of Illinois at Urbana-Champaign 61801, USA.
MeSH Terms
- Animals
- Bacteroides / classification
- Bacteroides / genetics
- Bacteroides / metabolism
- Base Sequence
- Cecum / microbiology
- Cellulose / metabolism
- DNA Primers / genetics
- DNA Probes / genetics
- Horses / microbiology
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Phylogeny
- Polymerase Chain Reaction
- RNA, Bacterial / genetics
- RNA, Ribosomal, 16S / genetics
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