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Home / Equine Research Bank / Chromosoma / The catalytic and the RNA subunits of human telomerase are r...
Chromosoma2012; 121(5); 475-488; doi: 10.1007/s00412-012-0379-4

The catalytic and the RNA subunits of human telomerase are required to immortalize equid primary fibroblasts.

Abstract: Many human primary somatic cells can be immortalized by inducing telomerase activity through the exogenous expression of the human telomerase catalytic subunit (hTERT). This approach has been extended to the immortalization of cell lines from several mammals. Here, we show that hTERT expression is not sufficient to immortalize primary fibroblasts from three equid species, namely donkey, Burchelli's zebra and Grevy's zebra. In vitro analysis of a reconstituted telomerase composed by hTERT and an equid RNA component of telomerase (TERC) revealed a low activity of this enzyme compared to human telomerase, suggesting a low compatibility of equid and human telomerase subunits. This conclusion was also strengthened by comparison of human and equid TERC sequences, which revealed nucleotide differences in key regions for TERC and TERT interaction. We then succeeded in immortalizing equid fibroblasts by expressing hTERT and hTERC concomitantly. Expression of both human telomerase subunits led to telomerase activity and telomere elongation, indicating that human telomerase is compatible with the other equid telomerase subunits and proteins involved in telomere metabolism. The immortalization procedure described herein could be extended to primary cells from other mammals. The availability of immortal cells from endangered species could be particularly useful for obtaining new information on the organization and function of their genomes, which is relevant for their preservation.
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Publication Date: 2012-07-14 PubMed ID: 22797876PubMed Central: PMC3443485DOI: 10.1007/s00412-012-0379-4Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research reveals that certain human telomerase parts can be combined with equivalent parts from three equid species to create immortal cell lines, providing implications for further genetic studies and species preservation.

Introduction

  • The study focuses on the possibility of immortalizing primary somatic cells from three equid species: donkeys, Burchelli’s zebras, and Grevy’s zebras.
  • Prior research has demonstrated that many human primary somatic cells can be immortalized by inducing telomerase activity, particularly through expressing the human telomerase catalytic subunit (hTERT).

Methodology

  • The researchers attempted to express hTERT in primary fibroblasts from the three equid species identified.
  • For in vitro analysis, they constructed a version of telomerase that included hTERT and an equid RNA component.

Research Findings

  • Initial attempts showed that hTERT expression was not sufficient to immortalize the equid fibroblasts uncovered in this study.
  • The in vitro analysis revealed that the reconstituted telomerase had a lower activity level compared with the native human telomerase, suggesting limited compatibility between human and equid telomerase components.
  • To further investigate the possible causes of this incompatibility, the researchers compared the human and equid variants of TERC, an RNA component of telomerase seen as critical for its interaction and function.
  • The comparison showed key nucleotide differences, providing further reason behind their incompatibility.

Immortalizing Equid Fibroblasts

  • Despite initial setbacks, the team managed to immortalize equid fibroblasts by expressing both hTERT and hTERC at the same time.
  • This concurrent expression triggered telomerase activity and telomere elongation, suggesting that human telomerase is compatible with other equid telomerase components and key proteins that play a role in telomere maintenance.

Implications and Future Directions

  • The successful immortalization of equid fibroblasts opens doors for the immortalization of primary cells from other mammal species as well.
  • The availability of immortal cells from endangered species could be particularly beneficial for obtaining new information related to genome structure and function in these beings, thereby contributing to their conservation.

Cite This Article

APA
Vidale P, Magnani E, Nergadze SG, Santagostino M, Cristofari G, Smirnova A, Mondello C, Giulotto E. (2012). The catalytic and the RNA subunits of human telomerase are required to immortalize equid primary fibroblasts. Chromosoma, 121(5), 475-488. https://doi.org/10.1007/s00412-012-0379-4

Publication

Chromosoma
ISSN: 1432-0886
NlmUniqueID: 2985138R
Country: Austria
Language: English
Volume: 121
Issue: 5
Pages: 475-488

Researcher Affiliations

Vidale, Pamela
  • Dipartimento di Biologia e Biotecnologie Lazzaro Spallanzani, Università degli Studi di Pavia, Via Ferrata 1, 27100, Pavia, Italy.
Magnani, Elisa
    Nergadze, Solomon G
      Santagostino, Marco
        Cristofari, Gael
          Smirnova, Alexandra
            Mondello, Chiara
              Giulotto, Elena

                MeSH Terms

                • Animals
                • Base Sequence
                • Catalytic Domain
                • Cells, Cultured
                • Equidae
                • Fibroblasts / cytology
                • Fibroblasts / metabolism
                • Horses
                • Humans
                • Mice
                • Molecular Sequence Data
                • Protein Subunits / chemistry
                • Protein Subunits / genetics
                • Protein Subunits / metabolism
                • RNA / chemistry
                • RNA / genetics
                • RNA / metabolism
                • Telomerase / chemistry
                • Telomerase / genetics
                • Telomerase / metabolism
                • Telomere / genetics
                • Telomere / metabolism
                • Transfection

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                Citations

                This article has been cited 10 times.
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