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Home / Equine Research Bank / Equine Vet J / The effect of medical grade honey on tensile strength, strai...
Equine veterinary journal2023; doi: 10.1111/evj.13966

The effect of medical grade honey on tensile strength, strain, and Young’s modulus of synthetic absorbable suture material used in equine surgery.

Abstract: Medical grade honey (MGH) has been introduced as a protective measure against surgical site infections in equine surgery, but the effect on suture material has not been investigated. Objective: To evaluate the effect of MGH on the tensile properties of three synthetic absorbable suture materials. Methods: In vitro experiments. Methods: Ten strands of Polydioxanone USP 2 (PD2), Polyglactin 910 USP 2 (PG2) and Polyglecaprone USP 2-0 (PC2-0) were incubated 1, 7, 14, 21 and 28 days in MGH, phosphate-buffered saline (PBS), equine plasma (EP) and MGH mixed with equine plasma (HP). Mechanical testing was performed recording maximum load at failure (N), strain at failure and Young's modulus (N/mm ). The results were reported from a Welch or regular ANOVA. Results: PD2 in MGH had a significantly higher tensile strength compared to PD2 in EP (p < 0.05, mean difference (md) = 16.95 N, CI95% (9.19-24.70 N)) and PBS (p < 0.05, md = 14.48 N CI95% (6.73-22.23 N)) on day 7 and the remaining time points. PG2 incubated in MGH had a significantly higher tensile strength compared to EP (p < 0.05, md = 69.28 N CI95% (64.16-74.40 N)) and PBS (p < 0.05, md = 56.90 N CI95% (51.78-62.02 N)) until day 28. PC2-0 incubated in MGH had a significantly higher tensile strength compared to EP (p < 0.05 md = 12.40 N CI95% (4.59 N-20.20 N)) and PBS (p < 0.05 md = 11.23 CI95% (3.42-19.03 N)) on day 7 and the remaining time points. Conclusions: The sutures were unloaded during incubation and a single cycle to failure test was only performed, which does not reflect the in vivo environment where shear forces are present. Conclusions: MGH did not negatively affect the tensile strength of the suture material and can safely be applied in contact with suture materials commonly used in equine surgery. Unassigned: Medizinischer Honig (Medical Grade Honey, MGH) wurde in der Pferdechirurgie als Schutzmaßnahme gegen Infektionen an der Operationsstelle eingeführt, aber die Auswirkungen auf das Nahtmaterial wurden noch nicht untersucht. ZIEL: Bewertung der Auswirkungen von MGH auf die Zugeigenschaften von drei synthetischen resorbierbaren Nahtmaterialien. Methods: In vitro experimente. Methods: Zehn Stränge von Polydioxanon USP 2 (PD2), Polyglactin 910 USP 2 (PG2) und Polyglecapron USP 2-0 (PC2-0) wurden 1, 7, 14, 21 und 28 Tage in MGH, phosphatgepufferter Kochsalzlösung (PBS), Pferdeplasma (EP) und MGH gemischt mit Pferdeplasma (HP) inkubiert. Bei den mechanischen Prüfungen wurden die maximale Bruchlast (N), die Bruchdehnung und der Elastizitätsmodul (Youngscher modul; N/mm ) aufgezeichnet. Die Ergebnisse wurden anhand einer Welch- oder regulären ANOVA ermittelt. Unassigned: PD2 in MGH hatte eine signifikant höhere Zugfestigkeit im Vergleich zu PD2 in EP (p < 0.05, md = 16.95 N, CI95% (9.19 N bis 24.70 N)) und PBS (p < 0.05, md = 14.48 N CI95% (6.73 N bis 22.23 N)) an Tag 7 und den übrigen Zeitpunkten. PG2, das in MGH bebrütet wurde, hatte bis zum 28. Tag eine signifikant höhere Zugfestigkeit als EP (p < 0.05, md = 69.28 N CI95% (64.16 N bis 74.40 N)) und PBS (p < 0.05, md = 56.90 N CI95% (51.78 N bis 62.02 N)). PC2-0, das in MGH inkubiert wurde, hatte eine signifikant höhere Zugfestigkeit im Vergleich zu EP (p < 0.05 md = 12.40 N CI95% (4.59 N bis 20.20 N)) und PBS (p < 0.05 md = 11.23 CI95% (3.42 N bis 19.03 N)) an Tag 7 und den übrigen Zeitpunkten. Unassigned: Die Nähte wurden unbelastet inkubiert, und es wurde nur ein einziger Testzyklus bis zum Versagen durchgeführt, was nicht der Umgebung in vivo entspricht, in der Scherkräfte vorhanden sind. Unassigned: MGH hat keinen negativen Einfluss auf die Zugfestigkeit des Nahtmaterials und kann sicher in Kontakt mit den üblicherweise in der Pferdechirurgie verwendeten Nahtmaterialien benutzt werden.
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Publication Date: 2023-06-16 PubMed ID: 37326536DOI: 10.1111/evj.13966Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the effects of medical grade honey (MGH) on the tensile strength of three synthetic absorbable suture materials used in equine surgery. The results indicate that MGH does not negative affect the tensile strength of these materials and can be safely applied in contact with them.

Objective and Methods of Research

  • The aim of this research was to evaluate the impact of MGH on the tensile properties of three synthetic absorbable suture materials: Polydioxanone USP 2 (PD2), Polyglactin 910 USP 2 (PG2) and Polyglecaprone USP 2-0 (PC2-0).
  • The materials were subjected to an in vitro experiment where they were incubated, for a range of days (1, 7, 14, 21, 28), in MGH, phosphate-buffered saline (PBS), equine plasma (EP) and a mix of MGH and equine plasma (HP).
  • The properties evaluated included maximum load at failure, strain at failure and Young’s modulus, which measures the stiffness of the material.

Results of the Research

  • The results were reported using a Welch or regular ANOVA statistical tests.
  • Among various findings, one key observation was that PD2 in MGH had a significantly higher tensile strength compared to PD2 in EP and PBS on day 7 and the remaining time points.
  • Similarly, PG2 and PC2-0 incubated in MGH also showed significantly higher tensile strength compared to when they were incubated in EP and PBS.

Conclusions and Implications

  • The researchers note that the sutures underwent a single cycle of load at failure during incubation, which is not representative of the in vivo environment where constant shear forces are present.
  • Despite this limitation, the study concludes that MGH did not negatively affect the tensile strength of the suture material. This indicates that MGH can safely be applied in contact with suture materials commonly used in equine surgery.
  • This suggests that the use of MGH as a protective measure against surgical site infections in equine surgery could potentially be extended without negative impacts on suture material strength.

Cite This Article

APA
Madsen K, Martens A, Haspeslagh M, Meulyzer M, Gustafsson K. (2023). The effect of medical grade honey on tensile strength, strain, and Young’s modulus of synthetic absorbable suture material used in equine surgery. Equine Vet J. https://doi.org/10.1111/evj.13966

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English

Researcher Affiliations

Madsen, Kira
  • Morette Equine Hospital, Asse, Belgium.
Martens, Ann
  • Faculty of Veterinary Medicine, Department of Large Animal Surgery, Anaesthesia and Orthopaedic, Ghent University, Merelbeke, Belgium.
Haspeslagh, Maarten
  • Faculty of Veterinary Medicine, Department of Large Animal Surgery, Anaesthesia and Orthopaedic, Ghent University, Merelbeke, Belgium.
Meulyzer, Michael
  • Morette Equine Hospital, Asse, Belgium.
Gustafsson, Kajsa
  • Department of Veterinary Medicine and Animal Science (DIVAS), University of Milan, Lodi, Italy.

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