The effects of different types of syringes on equine spermatozoa.
Abstract: Control extender was incubated at 4 degrees C for 24 hours. Rubber or plastic syringe plungers were separately incubated in semen extender for 24 hours at 4 degrees C. Following incubation, the extender was stored at -20 degrees C until the time of semen collection. The treatments consisted of the following: Group A = equine semen plus control extender; Group B=equine semen plus extender incubated with rubber plungers and Group C=equine semen plus extender incubated in plastic plungers; Group D=equine semen plus control extended in rubber plunger syringes and Group E=equine semen plus control extender in plastic plunger syringer. Each group contained a 5-ml volume of semen and extender at a concentration of 1.0 x 10(8) sperm/ml. The number of live spermatozoa, percentage of progressively motile spermatozoa and rate of progressive motility were taken following collection and every 15 minutes for 1 hour following application of treatments. In experiment 2, treatments were allowed to incubate with semen for 45 minutes, then the extender was removed and was replaced with fresh extender. The rate of progressive motility and the percentage of progressively motile spermatozoa were taken immediately, at 45 minutes, and then every 15 minutes for 1 hour. In experiment 1, the number of live spermatozoa was not affected among the 5 groups. However, there was a decrease (P<0.01) in the rate of progressive motility and in the percentage of progressively motile spermatozoa in Group B compared with the remaining 4 treatment groups at 30, 45 and 60 minutes, with no differences noted when semen was held in syringes with a rubber or a plastic plunger. In experiment 2, the percentage of progressively motile spermatozoa increased after the addition of the control extender.
Publication Date: 1993-02-01 PubMed ID: 16727219DOI: 10.1016/0093-691x(93)90382-fGoogle Scholar: Lookup
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- Journal Article
Summary
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The research looks at the effect of different types of syringes, particularly the materials (rubber or plastic) used on their plungers, on the vitality and motility of equine spermatozoa.
Research Methodology
- The experiment involved a control extender, a medium used to preserve the sperm, which was incubated at a temperature of 4 degrees Celsius for 24 hours.
- Two other types of extenders were also incubated at the same temperature but interfered by rubber and plastic plungers for 24 hours. After incubation, these extenders were frozen at -20 degrees celsius until the time of semen collection.
- The researchers created five experimental groups: Group A, B, C, D, and E. Group A used equine semen and the control extender. Group B incorporated semen and the extender incubated with rubber plungers. Group C used semen and the extender incubated with plastic plungers. Group D combined semen and control extender in rubber plunger syringes while Group E utilized semen and control extender stored in plastic plunger syringes. Each group contained a 5-ml volume of semen and extender at a concentration of 1.0 x 10(8) sperm/ml.
- Three major parameters of sperm health were closely monitored: the number of live spermatozoa, the percentage of progressively motile spermatozoa (the ability to move actively and linearly), and the rate of progressive motility. These parameters were taken immediately after collection and every 15 minutes for 1 hour following application of treatments.
Findings
In experiment 1:
- The vitality of spermatozoa, represented by the number of live cells, was not affected among different groups.
- However, the percentage and rate of progressively motile spermatozoa decreased significantly in Group B (sperm and extender incubated with rubber plungers) compared with the remaining 4 groups at 30, 45 and 60 minutes. There was no difference noted when the semen was stored in syringes using either rubber or plastic plunger.
In experiment 2:
- The researchers added fresh extender to the semen after an incubation period and noted an increase in the percentage of the progressively motile spermatozoa.
Conclusion
- The overall results suggest that the type of syringe used (with respect to the material of the plunger) could potentially have an impact on the health of equine spermatozoa, specifically on their motility.
Cite This Article
APA
Broussard JR, Goodeaux SD, Goodeaux LL, Thibodeaux JK, Moreau JD, Godke RA, Roussel JD.
(1993).
The effects of different types of syringes on equine spermatozoa.
Theriogenology, 39(2), 389-399.
https://doi.org/10.1016/0093-691x(93)90382-f Publication
Researcher Affiliations
- Department of Dairy, LAES, LSU Agricultural Center Louisiana State University, Baton Rouge, LA 70803 USA.
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