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Theriogenology2007; 68(3); 423-428; doi: 10.1016/j.theriogenology.2007.04.039

The efficient use of equine cryopreserved semen.

Abstract: In order to optimize the efficient use of cryopreserved stallion semen, recent research has focused on the minimum insemination dose of frozen-thawed spermatozoa required for maximum fertility rate. The results appear to be highly stallion-dependent. Factors such as the timing of AI with respect to ovulation, as well as the site of insemination within the mare's reproductive tract, also affect success in breeding with frozen-thawed semen. Since acceptable pregnancy rates can be achieved from insemination of mares with very low numbers of spermatozoa, increasing the number of insemination doses processed from a single ejaculate may prove more cost-effective to stallion owners.
Publication Date: 2007-06-22 PubMed ID: 17588649DOI: 10.1016/j.theriogenology.2007.04.039Google Scholar: Lookup
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  • Journal Article

Summary

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This research article focuses on how to optimize the use of cryopreserved (frozen) stallion semen for horse breeding, with considerations including the minimum effective insemination dose, timing of AI relative to ovulation, and insemination location within the mare’s reproductive tract. The findings suggest that adjusting these factors could improve the efficiency and potentially the cost-effectiveness of using frozen semen.

Optimizing Use of Cryopreserved Stallion Semen

This research paper discusses the efficient use of cryopreserved, or frozen, stallion semen for artificial insemination in horse breeding. The primary cause of interest is finding the minimum insemination dose required for the highest fertility rate. The points discussed include:

  • Spermatozoa Dose: Researchers explored the least amount of frozen-thawed sperm cells needed for maximum fertility. This is a critical aspect as it directly impacts the number of possible insemination doses obtained from a single ejaculate, from a cost-efficiency point of view.
  • Stallion Dependence: The study emphasizes that these results are contingent on the particular stallion. This suggests that different stallions have varying effectiveness rates with the use of cryopreserved semen, meaning a tailored approach may be necessary.
  • AI and Ovulation Timing: The timing of artificial insemination (AI) with respect to the mare’s ovulation also plays a crucial role in successful breeding. Knowing when a mare is most fertile can help in determining the best time to inseminate for optimal results.
  • Insemination Site: Where within the mare’s reproductive tract the semen is introduced also affects the likelihood of successful insemination. Precise positioning can benefit successful fertilization and subsequent pregnancy.

Implications of the Research

The findings of this research can enhance the efficiency and reduce the costs linked with horse breeding using cryopreserved stallion semen. By figuring out the least amount of spermatozoa needed for successful insemination, breeders can potentially get more use out of a single ejaculate. Moreover, understanding how the timing of AI and the location of insemination affect fertility rates can pave the way for more controlled and predicted breeding outcomes. In short, this research aims to make the use of frozen semen a more accessible and viable option for horse breeders.

Cite This Article

APA
Metcalf ES. (2007). The efficient use of equine cryopreserved semen. Theriogenology, 68(3), 423-428. https://doi.org/10.1016/j.theriogenology.2007.04.039

Publication

ISSN: 0093-691X
NlmUniqueID: 0421510
Country: United States
Language: English
Volume: 68
Issue: 3
Pages: 423-428

Researcher Affiliations

Metcalf, E S
  • Honahlee PC, Sherwood, OR, USA. honahlee@imagina.com

MeSH Terms

  • Animals
  • Breeding
  • Cryopreservation
  • Female
  • Horses / physiology
  • Insemination, Artificial / methods
  • Male
  • Pregnancy
  • Pregnancy Rate
  • Semen / physiology
  • Semen Preservation
  • Time Factors

Citations

This article has been cited 2 times.
  1. Gil L, Galindo-Cardiel I, Malo C, González N, Alvarez C. Effect of Cholesterol and Equex-STM Addition to an Egg Yolk Extender on Pure Spanish Stallion Cryopreserved Sperm. ISRN Vet Sci 2013;2013:280143.
    doi: 10.1155/2013/280143pubmed: 24416597google scholar: lookup
  2. Gutiérrez-Cepeda L, Fernández A, Crespo F, Ramírez MÁ, Gosálvez J, Serres C. The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA. Acta Vet Scand 2012 Dec 5;54(1):72.
    doi: 10.1186/1751-0147-54-72pubmed: 23217215google scholar: lookup