The fluorescent antibody technique for diagnosis of equine histoplasmosis (epizootic lymphangitis).
Abstract: Summary
The fluorescent antibody technique (FA) was evaluated as a diagnostic aid for the diagnosis of equine histoplasmosis, using sera from ten clinical cases of epizootic lymphangitis. The globulin fraction was separated, quantitated and then labelled with fluorescein isothiocynate. Of several antigen fixatives, heat-fixation was the best. Conjugated sera diluted 1:5 yielded the highest fluorescence intensity with heat-fixed mycelial antigen preparations. Acetone and P-formaldehyde treated preparations gave a less efficient intensity. Glutaraldehyde was the least effective fixative.
The test confirmed that humoral antibody formation occurs during the course of the infection and the results seem promising for the use of FA as a diagnostic aid.
Zusammenfassung
Die Benutzung der fluoreszierenden Antikörpertechnik für den Nachweis von equiner Histoplasmose (epizootischer Lymphangitis)
Die fluoreszierende Antikörpertechnik wurde erstmals zum Nachweis der Histoplasmose der Pferde (epizootische Lymphangitis) herangezogen. Die Seren stammten von 10 klinischen Fällen. Die Globulinfraktion wurde abgetrennt und mit Fluorescein-Isothiocyanat markiert.
Mehrere Methoden der Antigenfixierung wurden ausprobiert. Am besten geeignet war die Hitzefixation. 1: 5 verdünnte konjugierte Seren zeigten die höchste Fluoreszenz-aktivität mit hitzefixierten Antigenpräparationen aus dem Mycel. Mit Azeton und p-Formaldehyd behandelte Präparationen ergaben eine schlechtere Intensität, Glutaraldehyd war am wenigsten geeignet.
Während der Infektion kommt es zur Bildung humoraler Antikörper, die mit dem Test nachweisbar waren. Die erzielten Ergebnisse lassen den Test als geeignet für die Diagnose erscheinen.
Résumé
La technique des anticorps fluorescents pour le diagnostic de l'histoplasmose équine (Lymphangite épizootique)
La technique des anticorps fluorescents a été utilisée pour la première fois dans la mise en évidence de l'histoplasmose chez les chevaux (lymphangite épizootique). Les sérums provenaient de 10 cas cliniques. La fraction des globulines a été séparée et marquée avec l'isothiocyanate de fluorescéine.
Différentes méthodes de fixation de l'antigène ont été testées. La fixation thermique fut la plus appropriée. Des sérums conjugés dilués 1: 5 ont montré la plus forte activité fluorescente avec des préparations d'antigène fixées à la chaleur à partir du mycèle. Des préparations traitées à l'acétone et au p-formaldéhyde ont rendu une intensité moins bonne et le glutaraldéhyde fut le moins approprié.
II est apparu pendant l'infection une formation d'anticorps humoraux qui ont été mis en évidence par le test. Les résultats obtenus laissent apparaître que le test est valable pour le diagnostic.
Resumen
El uso de la técnica de anticuerpos fluorescentes en el diagnóstico de la histoplasmosis equina (linfangitis epizoótica)
Por vez primera se recurrió a la técnica de anticuerpos fluorescentes para la puesta en evidencia de la histoplasmosis de los caballos (linfangitis epizoótica). Los sueros sanguíneos procedían de 10 casos clínicos. Se separó la fracción globulínica, marcándose con isotiocianato de fluoresceína.
Se probaron varias técnicas para la fijación del antígeno. La más adecuada era la fijación térmica. Sueros conjugados, diluidos a 1: 5, mostraron la actividad de fluorescencia máxima con preparaciones de antígeno miceliar fijadas con el calor. Las preparaciones tratadas con acetona y p-formaldehido acusaron una intensidad peor, mientras que el glutaraldehido era el menos apropiado para este fin.
Durante la infección se forman anticuerpos humorales, identificables con la prueba. Los resultados obtenidos indican que la prueba es adecuada para establecer el diagnóstico.
Publication Date: 1983-05-01 PubMed ID: 6346753DOI: 10.1111/j.1439-0450.1983.tb01844.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The researchers have conducted a study to assess the effectiveness of the fluorescent antibody technique (FA) as a diagnostic tool for equine histoplasmosis, a highly infectious fungal disease that affects horses. This was done by testing the sera from ten cases of epizootic lymphangitis, another horse disease that can be caused by the same fungus. The researched technique has shown promise in identifying antibodies produced during the infection, indicating that it could be used for diagnosis in the future.
Methodology and Findings
- The study involved testing sera from ten clinical cases of epizootic lymphangitis, a condition that can be caused by Histoplasma capsulatum, the fungus responsible for equine histoplasmosis.
- The globulin fraction from the sera was separated and labelled with a fluorescent dye called fluorescein isothiocynate. This process is the basis for the fluorescent antibody technique, which involves using fluorescently labelled antibodies to detect the presence of certain antigens.
- The researchers compared several methods of antigen fixation, a step in the process that involves making the antigens (in this case, elements of the fungus causing the disease) stay in place so they can be detected by the antibodies. It was found that heat-fixation was the most effective method. Other methods, such as using acetone, P-formaldehyde, and glutaraldehyde, were less effective.
- The conjugated sera (sera in which the antibodies have been labelled with a fluorescent dye) were diluted to 1:5, and this dilution resulted in the highest fluorescence intensity with the heat-fixed antigen preparations.
- The researchers found evidence of humoral antibody formation during the infection, confirming that the body’s immune system produces these antibodies in response to the infection. This finding supports the use of the fluorescent antibody technique for diagnosis, since the presence of these antibodies can be detected using this method.
Conclusion
- The fluorescent antibody technique showed promise as a diagnostic tool for equine histoplasmosis, as it was able to detect the presence of antibodies produced in response to the infection.
- This suggests it could be used as a diagnostic aid in the future, though further testing and validation would likely be necessary before it could be widely implemented.
Cite This Article
APA
Gabal MA, Bana AA, Gendi ME.
(1983).
The fluorescent antibody technique for diagnosis of equine histoplasmosis (epizootic lymphangitis).
Zentralbl Veterinarmed B, 30(4), 283-287.
https://doi.org/10.1111/j.1439-0450.1983.tb01844.x Publication
Researcher Affiliations
MeSH Terms
- Animals
- Antibodies, Fungal / analysis
- Fluorescent Antibody Technique
- Histoplasma / immunology
- Histoplasmosis / diagnosis
- Histoplasmosis / veterinary
- Horse Diseases / diagnosis
- Horses
- Lymphangitis / diagnosis
- Lymphangitis / veterinary
Citations
This article has been cited 0 times.Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
