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Home / Equine Research Bank / Anat Embryol (Berl) / The immunocytochemical distribution of seven peptides in the...
Anatomy and embryology1990; 181(3); 271-280; doi: 10.1007/BF00174620

The immunocytochemical distribution of seven peptides in the spinal cord and dorsal root ganglia of horse and pig.

Abstract: The distribution of calcitonin gene-related peptide (CGRP), enkephalin, galanin, neuropeptide Y (NPY), somatostatin, tachykinins and vasoactive intestinal polypeptide (VIP) was compared in cervical, thoracic, lumbar and sacral segmental levels of spinal cord and dorsal root ganglia of horse and pig. In both species, immunoreactivity for the peptides under study was observed at all segmental levels of the spinal cord. Peptide-immunoreactive fibres were generally concentrated in laminae I-III, the region around the central canal, and in the autonomic nuclei. A general increase in the number of immunoreactive nerve fibres was noted in the lumbosacral segments of the spinal cord, which was particularly exaggerated in the case of VIP immunoreactivity. In the horse, some CGRP-, somatostatin- or tachykinin-immunoreactive cell bodies were present in the dorsal horn. In the pig, cells immunoreactive for somatostatin, enkephalin or NPY were noted in a similar location. In the ventral horn most motoneurones were CGRP-immunoreactive in both species. However, in pig many other cell types were CGRP-immunoreactive not only in the ventral horn, but also in laminae V-VI of the dorsal horn. With the exception of enkephalin and NPY immunoreactivity, which was not seen in pig dorsal root ganglia, all peptides studied were localised to neuronal cell bodies and/or fibres in the dorsal root ganglia. In both species, immunolabeled cell bodies were observed in ganglia from cervical, thoracic, lumbar and sacral levels, with the exception of VIP-immunoreactive cells that were detected only in the lumbosacral ganglia. Numerous CGRP- and tachykinin-immunoreactive cell bodies were visualised in both species, while the cells immunolabeled with other peptide antisera were much lower in number. In both species, immunostaining of serial sections revealed that a subset of CGRP-immunoreactive cells co-expressed tachykinin, galanin or somatostatin immunoreactivity. In the horse some enkephalin-immunoreactive cells were also CGRP positive and occasionally combinations of three peptides, e.g. CGRP, tachykinin and galanin or CGRP, tachykinin and enkephalin were identified. The results obtained suggest that the overall pattern of distribution of peptide immunoreactivities is in general agreement with that so far described in other mammals, although some species variations have been observed, particularly regarding the presence of immunoreactive cell bodies in the dorsal horn of the spinal cord.
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Publication Date: 1990-01-01 PubMed ID: 1692451DOI: 10.1007/BF00174620Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the distribution of seven peptides in the spinal cord and dorsal root ganglia in horses and pigs, revealing various similarities, differences, and species-specific variations.

Peptides Studied and Their Distribution

  • The research focused on seven peptides – calcitonin gene-related peptide (CGRP), enkephalin, galanin, neuropeptide Y (NPY), somatostatin, tachykinins, and vasoactive intestinal polypeptide (VIP).
  • The distribution was observed in different segmental levels of the spinal cord – cervical, thoracic, lumbar, and sacral, and also in the dorsal root ganglia of both horses and pigs.
  • It was noted that immunoreactivity for these peptides existed in all segmental levels in both species. This reactivity usually concentrated around the region of the central canal, autonomic nuclei, and in laminae I-III.
  • The lumbosacral segments generally had an increased number of peptide-immunoreactive nerve fibres, with VIP immunoreactivity being noticeably above average.

Species Differences in Cell Bodies

  • In horses, the dorsal horn showed CGRP-, somatostatin-, or tachykinin-immunoreactive cell bodies. In pigs, similar locations had cells immune-reactive for somatostatin, enkephalin, or NPY.
  • Most motoneurons in the ventral horn showed CGRP-immunoreactivity in both species. However, many other cell types in pigs showed this same reactivity, especially in the ventral horn and laminae V-VI of the dorsal horn.

Comparison of Peptide Immunoreactivities in Dorsal Root Ganglia

  • Except for enkephalin and NPY which were not visible in pig dorsal root ganglia, the other peptides were localised in neuronal cell bodies and/or fibres in the region.
  • Immunolabeled cell bodies, including VIP-immunoreactive cells, were present in cervical, thoracic, lumbar and sacral ganglia. However, VIP-immunoreactive cells were seen only in lumbosacral ganglia.
  • A significant number of CGRP- and tachykinin-immunoreactive cells were observed in both species, but cells immune-labeled with other peptides were found in lower quantities.

Co-Expression of Peptides

  • Immunostaining of serial sections revealed subsets of CGRP-immunoreactive cells expressing tachykinin, galanin, or somatostatin as well in both animals.
  • In horses, some enkephalin-immunoreactive cells were also CGRP positive. Occasionally, cells exhibited three peptides, such as CGRP, tachykinin, and galanin or CGRP, tachykinin, and enkephalin.

Conclusion

  • The overall immunoreactive pattern observed for the seven peptides is generally in line with what is seen in other mammals. Nevertheless, there were some species-specific variations, especially regarding the presence of immunoreactive cell bodies in the dorsal horn of the spinal cord.

Cite This Article

APA
Merighi A, Kar S, Gibson SJ, Ghidella S, Gobetto A, Peirone SM, Polak JM. (1990). The immunocytochemical distribution of seven peptides in the spinal cord and dorsal root ganglia of horse and pig. Anat Embryol (Berl), 181(3), 271-280. https://doi.org/10.1007/BF00174620

Publication

Anatomy and embryology
ISSN: 0340-2061
NlmUniqueID: 7505194
Country: Germany
Language: English
Volume: 181
Issue: 3
Pages: 271-280

Researcher Affiliations

Merighi, A
  • Department of Histochemistry, Royal Postgraduate Medical School, London, United Kingdom.
Kar, S
    Gibson, S J
      Ghidella, S
        Gobetto, A
          Peirone, S M
            Polak, J M

              MeSH Terms

              • Animals
              • Calcitonin Gene-Related Peptide / metabolism
              • Enkephalins / metabolism
              • Galanin
              • Ganglia, Spinal / metabolism
              • Horses
              • Immunohistochemistry
              • Neuropeptide Y / metabolism
              • Neuropeptides / metabolism
              • Peptides / metabolism
              • Somatostatin / metabolism
              • Spinal Cord / metabolism
              • Swine
              • Tachykinins / metabolism
              • Vasoactive Intestinal Peptide / metabolism

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