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Home / Equine Research Bank / Int J Mol Sci / The Induced Expression of BPV E4 Gene in Equine Adult Dermal...
International journal of molecular sciences2022; 23(4); 1970; doi: 10.3390/ijms23041970

The Induced Expression of BPV E4 Gene in Equine Adult Dermal Fibroblast Cells as a Potential Model of Skin Sarcoid-like Neoplasia.

Abstract: The equine sarcoid is one of the most common neoplasias in the family. Despite the association of this tumor with the presence of bovine papillomavirus (BPV), the molecular mechanism of this lesion has not been fully understood. The transgenization of equine adult cutaneous fibroblast cells (ACFCs) was accomplished by nucleofection, followed by detection of molecular modifications using high-throughput NGS transcriptome sequencing. The results of the present study confirm that - and -mediated nucleofection strategy significantly affected the transcriptomic alterations, leading to sarcoid-like neoplastic transformation of equine ACFCs. Furthermore, the results of the current investigation might contribute to the creation of in vitro biomedical models suitable for estimating the fates of molecular dedifferentiability and the epigenomic reprogrammability of and transgenic equine ACFC-derived sarcoid-like cell nuclei in equine somatic cell-cloned embryos. Additionally, these in vitro models seem to be reliable for thoroughly recognizing molecular mechanisms that underlie not only oncogenic alterations in transcriptomic signatures, but also the etiopathogenesis of epidermal and dermal sarcoid-dependent neoplastic transformations in horses and other equids. For those reasons, the aforementioned transgenic models might be useful for devising clinical treatments in horses afflicted with sarcoid-related neoplasia of cutaneous and subcutaneous tissues.
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Publication Date: 2022-02-10 PubMed ID: 35216085PubMed Central: PMC8877312DOI: 10.3390/ijms23041970Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the role of bovine papillomavirus (BPV) in equine sarcoid, a common type of skin tumor in horses. This was achieved using molecular techniques to introduce BPV genes into horse skin cells and analyze how their genetic profile is altered, potentially leading to a better understanding of how the tumor develops, and creating models for future research and treatment strategies.

Objective of the study

  • The main objective of this research was to understand the molecular mechanism of equine sarcoid, a common skin tumor in horses that is associated with the bovine papillomavirus (BPV).
  • The authors hoped to achieve this by introducing the BPV genes into the cells of horse skin (adult cutaneous fibroblast cells) and examining how this affected their genetic profile.

Methodology

  • The researchers used a method called ‘nucleofection’ to introduce the BPV genes into the horse skin cells. This method involves using an electric pulse to create temporary pores in the cell membrane, which allows DNA to enter the cells.
  • After the BPV genes had been introduced, the cells’ molecular structure was analyzed using high-throughput next-generation sequencing (NGS) technology.

Results

  • The study found that nucleofection of horse skin cells with BPV significantly altered their genetic profile and led to sarcoid-like (tumor-like) transformations.
  • This suggests that BPV plays an important role in causing skin tumors in horses, potentially by altering their genetic structure.

Significance

  • The results of the study contribute to the creation of in vitro models (laboratory models using cells or biological molecules), which can be used to study the changes in genetic profile associated with horse skin tumors, and to explore the reprogramming of the cells due to BPV.
  • This is of great significance as these models can also be used to study the mechanisms of other neoplastic transformations (changes leading to tumour formation) in horses and equids, helping to gain a better understanding of the disease and in turn, helping devise better clinical treatments.

Cite This Article

APA
Podstawski P, Samiec M, Skrzyszowska M, Szmatoła T, Semik-Gurgul E, Ropka-Molik K. (2022). The Induced Expression of BPV E4 Gene in Equine Adult Dermal Fibroblast Cells as a Potential Model of Skin Sarcoid-like Neoplasia. Int J Mol Sci, 23(4), 1970. https://doi.org/10.3390/ijms23041970

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 23
Issue: 4
PII: 1970

Researcher Affiliations

Podstawski, Przemysław
  • Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland.
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza 24/28, 30-059 Kraków, Poland.
Samiec, Marcin
  • Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland.
Skrzyszowska, Maria
  • Department of Reproductive Biotechnology and Cryoconservation, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland.
Szmatoła, Tomasz
  • Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland.
  • Center for Experimental and Innovative Medicine, University of Agriculture in Krakow, Rędzina 1c Street, 30-248 Kraków, Poland.
Semik-Gurgul, Ewelina
  • Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland.
Ropka-Molik, Katarzyna
  • Department of Animal Molecular Biology, National Research Institute of Animal Production, Krakowska 1 Street, 32-083 Balice, Poland.

MeSH Terms

  • Animals
  • Animals, Genetically Modified / virology
  • Equidae / virology
  • Fibroblasts / virology
  • Horse Diseases / virology
  • Horses / virology
  • Neoplasms / virology
  • Papillomaviridae / genetics
  • Papillomavirus Infections / virology
  • Sarcoidosis / virology
  • Skin / virology
  • Skin Diseases / virology
  • Transcriptome / genetics

Grant Funding

  • DI2016 012746 / Ministry of Science and Higher Education
  • 04-19-11-21 / National Research Institute of Animal Production

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

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Citations

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