The KIT is a putative marker for differentiating spermatogonia in stallions.
Abstract: Putative markers have been discovered and are used to identify and separate certain lineage of spermatogonia. The KIT is a marker for differentiating spermatogonial stem cells in several species including mice and goats. The objectives of this study were (1) to investigate reproductive stage-dependent KIT expression patterns in stallions and (2) to identify spermatogonia subpopulations expressing KIT in stallion testes. To achieve these objectives, testicular samples were obtained during routine field castration of stallions. The reproductive stage of the stallions was classified as pre-pubertal (<1 year, n=3), pubertal (1-1.5 year, n=4), post-pubertal (2-3 year, n=6), or adult (4-8 year, n=6). For immunohistochemistry, KIT was used at a dilution of 1:200. In the pre-pubertal and pubertal stage, most germ cells were immunolabeled with KIT. In the post-pubertal and adult stages, immunolabeling of KIT was evident in the germ cells attached to the basement membrane of the seminiferous tubules with exception of some spermatogonia. Co-immunolabeling with KIT and deleted in azoospermia like (DAZL) showed different co-staining patterns, including KIT only, both KIT and DAZL), or DAZL positive germ cell populations alone. The KIT was not immunolabeled in Sertoli or Leydig cells at any reproductive stages. The result of Western blot analysis verified the cross-activity of the KIT antibody with horse testes tissue. In conclusion, KIT appears to be expressed in differentiating spermatogonia, and may be used to identify and isolate differentiating germ cells from stallions.
Copyright © 2014 Elsevier B.V. All rights reserved.
Publication Date: 2014-11-21 PubMed ID: 25435078DOI: 10.1016/j.anireprosci.2014.11.004Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article is about the identification of KIT as a marker for differentiating spermatogonia in stallions at different reproductive stages.
Introduction and Study Objectives
- Markers are a way to identify and separate specific types of cells. One such marker, known as KIT, has been recognized for differentiating spermatogonial stem cells in various species, such as goats and mice.
- The study aimed to identify KIT’s expression patterns in stallions at different reproductive stages and to pinpoint spermatogonia subpopulations expressing KIT in stallion testes.
Methodology
- The researchers gathered testicular samples during routine field castration of stallions. The stallions were divided into four reproductive stages: pre-pubertal, pubertal, post-pubertal, and adult based on their age.
- KIT was used in a 1:200 dilution for immunohistochemistry, a process where antibodies, i.e., the KIT, bind to specific cells to help visualize and study them. The distribution of KIT in germ cells at different stages was observed.
Results
- In the pre-pubertal and pubertal stages, most germ cells were marked with KIT. In the later stages, post-pubertal and adult, KIT was observable in germ cells attached to the basement membrane of the seminiferous tubules, with some spermatogonia being exceptions.
- Double staining with KIT and DAZL (Deleted in Azoospermia-Like, another marker used primarily for germ cells in males) showed a variety of staining patterns, allowing for further differentiation between cell populations.
- KIT did not mark Sertoli or Leydig cells, distinct types of cells within the testes. This was consistent across all reproductive stages.
- Western blot analysis further verified the KIT antibody’s cross-activity with horse testes tissue, adding another layer of confirmation to the findings.
Conclusion
- Based on the study findings, KIT appears to be a noteworthy marker for differentiating spermatogonia in stallions. It may serve as a useful tool to identify and isolate differentiating germ cells in stallions, supporting advancements in reproductive biology and potentially enhancing breeding practices.
Cite This Article
APA
Jung H, Song H, Yoon M.
(2014).
The KIT is a putative marker for differentiating spermatogonia in stallions.
Anim Reprod Sci, 152, 39-46.
https://doi.org/10.1016/j.anireprosci.2014.11.004 Publication
Researcher Affiliations
- Department of Animal and Biotechnology Science, College of Animal Science, Kyungpook National University, Sangju 742-711, Republic of Korea.
- Department of Animal & Food Bioscience, Research Institute for Biomedical & Health Science, College of Biomedical & Health Science, Konkuk University, Chung-ju 380-701, Republic of Korea.
- Department of Animal and Biotechnology Science, College of Animal Science, Kyungpook National University, Sangju 742-711, Republic of Korea; Department of Horse, Companion and Wild Animal Science, College of Animal Science, Kyungpook National University, Sangju 742-711, Republic of Korea. Electronic address: mjyoon@knu.ac.kr.
MeSH Terms
- Animals
- Biomarkers
- Blotting, Western
- Gene Expression Regulation / physiology
- Horses / physiology
- Male
- Proto-Oncogene Proteins c-kit / genetics
- Proto-Oncogene Proteins c-kit / metabolism
- Spermatogonia / metabolism
- Staining and Labeling
- Testis / metabolism
Citations
This article has been cited 7 times.- Reifarth L, Körber H, Packeiser EM, Goericke-Pesch S. Detection of spermatogonial stem cells in testicular tissue of dogs with chronic asymptomatic orchitis.. Front Vet Sci 2023;10:1205064.
- Xia P, Ouyang S, Shen R, Guo Z, Zhang G, Liu X, Yang X, Xie K, Wang D. Macrophage-Related Testicular Inflammation in Individuals with Idiopathic Non-Obstructive Azoospermia: A Single-Cell Analysis.. Int J Mol Sci 2023 May 16;24(10).
- Yao B, An K, Kang Y, Tan Y, Zhang D, Su J. Reproductive Suppression Caused by Spermatogenic Arrest: Transcriptomic Evidence from a Non-Social Animal.. Int J Mol Sci 2023 Feb 27;24(5).
- Qin J, Huang T, Wang Z, Zhang X, Wang J, Dang Q, Cui D, Wang X, Zhai Y, Zhao L, Lu G, Shao C, Li S, Liu H, Liu Z. Bud31-mediated alternative splicing is required for spermatogonial stem cell self-renewal and differentiation.. Cell Death Differ 2023 Jan;30(1):184-194.
- Shakeel M, Yoon M. Functions of somatic cells for spermatogenesis in stallions.. J Anim Sci Technol 2022 Jul;64(4):654-670.
- Scarlet D, Handschuh S, Reichart U, Podico G, Ellerbrock RE, Demyda-Peyrás S, Canisso IF, Walter I, Aurich C. Sexual Differentiation and Primordial Germ Cell Distribution in the Early Horse Fetus.. Animals (Basel) 2021 Aug 17;11(8).
- Cruz A, Sullivan DB, Doty KF, Hess RA, Canisso IF, Reddi PP. Acrosomal marker SP-10 (gene name Acrv1) for staging of the cycle of seminiferous epithelium in the stallion.. Theriogenology 2020 Oct 15;156:214-221.
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