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BMC veterinary research2013; 9; 237; doi: 10.1186/1746-6148-9-237

The panorama of animal leptospirosis in Rio de Janeiro, Brazil, regarding the seroepidemiology of the infection in tropical regions.

Abstract: Leptospirosis is an important disease caused by various serovars of Leptospira sp. It can affect humans as well as domestic and wild animals; therefore, it has importance for public health, animal production, and wild species. The aim of this paper is to discuss the epidemiology of animal leptospirosis in Rio de Janeiro, Brazil, as a possible model for other tropical regions. In several studies conducted in the last 20 years, a total of 47 rats, 120 dogs, 875 cows, 695 horses, 1,343 goats, 308 sheep and 351 pigs from all regions of the state, in addition to 107 wild mammals and 73 golden-lion tamarins were tested (MAT) for anti-Leptospira antibodies. Results: Seroreactivity was frequent in all studied species, confirming that the infection is endemic in Rio de Janeiro. Serogroups Icterohaemorrhagiae and Sejroe were the most prevalent in urban and rural scenarios, respectively. This paper reviews the current knowledge on animal leptospirosis in Rio de Janeiro and describes important differences between urban versus rural cycles of the infection in various species. Conclusions: Identification of the prevailing serogroups and their reservoirs is essential for understanding agent-host-environment interactions under tropical conditions.
Publication Date: 2013-12-01 PubMed ID: 24289165PubMed Central: PMC4220826DOI: 10.1186/1746-6148-9-237Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article focuses on the prevalence and impact of leptospirosis in animals in Rio de Janeiro, Brazil, providing a model for understanding how the disease behaves in other tropical regions around the world.

Research Objective

The primary aim of the research was to examine the epidemiology of animal leptospirosis in Rio de Janeiro, Brazil. By assessing the spread and prevalence of the disease, the researchers hoped to provide a model for understanding similar patterns in other tropical regions.

Methodology

  • The researchers conducted an extensive study over a period of 20 years.
  • They collected data from a wide range of animal species: 47 rats, 120 dogs, 875 cows, 695 horses, 1,343 goats, 308 sheep, and 351 pigs. The study also included samples from 107 wild mammals and 73 golden-lion tamarins.
  • Each animal was tested for anti-Leptospira antibodies to determine whether it had been exposed to leptospirosis.

Results

  • Seroreactivity (positive response to the antibody test) was common in all species, indicating a high prevalence of leptospirosis in the state of Rio de Janeiro.
  • The specific types of leptospirosis identified varied between urban and rural environments. Icterohaemorrhagiae was commonly found in urban areas, while Sejroe was most prevalent in rural areas.

Conclusions

  • This study confirms the endemic nature of leptospirosis in Rio de Janeiro, impacting all tested species, and distinguishing the different cycles of the disease in various urban and rural species.
  • Identifying the most common serogroups of the disease and their animal reservoirs provides crucial insights into how the disease operates within Rio de Janeiro’s specific ecological conditions. This knowledge is relevant for the management of the disease and can possibly be applied as a model for other tropical regions.

Cite This Article

APA
Martins G, Lilenbaum W. (2013). The panorama of animal leptospirosis in Rio de Janeiro, Brazil, regarding the seroepidemiology of the infection in tropical regions. BMC Vet Res, 9, 237. https://doi.org/10.1186/1746-6148-9-237

Publication

ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 9
Pages: 237

Researcher Affiliations

Martins, Gabriel
  • Department of Microbiology and Parasitology, Laboratory of Veterinary Bacteriology, Universidade Federal Fluminense, Rio de Janeiro, Brazil. mipwalt@vm.uff.br.
Lilenbaum, Walter

    MeSH Terms

    • Animals
    • Brazil / epidemiology
    • Cattle / microbiology
    • Disease Reservoirs / microbiology
    • Dogs / microbiology
    • Goats / microbiology
    • Horses / microbiology
    • Leontopithecus / microbiology
    • Leptospira / physiology
    • Leptospirosis / epidemiology
    • Leptospirosis / veterinary
    • Prevalence
    • Rats / microbiology
    • Rural Population / statistics & numerical data
    • Seroepidemiologic Studies
    • Sheep / microbiology
    • Swine / microbiology
    • Tropical Climate
    • Urban Population / statistics & numerical data

    References

    This article includes 49 references
    1. Desvars A, Cardinale E, Michault A. Animal leptospirosis in small tropical areas. Epidemiol Infect 2011;139:167–188.
      doi: 10.1017/S0950268810002074pubmed: 20875197google scholar: lookup
    2. Tassinari WS, Pellegrini DCP, Sá CBP, Reis RB, Ko AI, Carvalho MS. Detection and modelling of case clusters for urban leptospirosis. Trop Med Int Health 2008;13:503–512.
    3. Hamond C, Martins G, Lawson-Ferreira R, Medeiros MA, Lilenbaum W. The role of horses in the transmission of leptospirosis in an urban tropical area. Epidemiol Infect 2012;141:33–35.
      pmc: PMC9152048pubmed: 22417781
    4. Martins G, Penna B, Hamond C, Leite RC-K, Silva A, Ferreira A, Brandão F, Oliveira F, Lilenbaum W. Leptospirosis as the most frequent infectious disease impairing productivity in small ruminants in Rio de Janeiro, Brazil. Trop Anim Health Prod 2012;44:773–777.
      doi: 10.1007/s11250-011-9964-4pubmed: 21898182google scholar: lookup
    5. Suepaul SM, Carrington CVF, Campbell M, Borde G, Adesiyun AA. Serovars of Leptospira isolated from dogs and rodents. Epidemiol Infect 2010;138:1059–1070.
      doi: 10.1017/S0950268809990902pubmed: 19811697google scholar: lookup
    6. Suepaul SM, Carrington CV, Campbell M, Borde G, Adesiyun AA. Seroepidemiology of leptospirosis in livestock in Trinidad. Trop Anim Health Prod 2011;43:367–375.
      doi: 10.1007/s11250-010-9698-8pubmed: 20953838google scholar: lookup
    7. Maciel EAP, de Carvalho ALF, Nascimento SF, de Matos RB, Gouveia EL, Reis MG, Ko AI. Household transmission of leptospira infection in urban slum communities. PLoS Negl Trop Dis 2008;2:e154.
    8. Martins G, Penna B, Lilenbaum W. The dog in the transmission of human leptospirosis under tropical conditions: victim or villain?. Epidemiol Infect 2012;140:207–208.
      doi: 10.1017/S0950268811000276pubmed: 21396149google scholar: lookup
    9. Lilenbaum W, Souza GN. Factors associated with bovine leptospirosis in Rio de Janeiro, Brazil. Res Vet Sci 2003;75:249–251.
      doi: 10.1016/S0034-5288(03)00114-0pubmed: 13129675google scholar: lookup
    10. Oliveira TV, Marinho DP, Costa Neto C, Kligerman DC. Climate variables, living conditions and the health of the population: leptospirosis in the city of Rio de Janeiro from 1996 to 2009. Ciênc Saúde Colet 2012;17:1569–1576.
      pubmed: 22699647
    11. Hochedez P, Rosine J, Théodose R, Abel S, Bourhy P, Picardeau M, Quénel P, Cabié A. Outbreak of leptospirosis after a race in the tropical forest of Martinique. Am J Trop Med Hyg 2011;84:621–626.
      doi: 10.4269/ajtmh.2011.10-0502pmc: PMC3062459pubmed: 21460020google scholar: lookup
    12. Robertson C, Nelson TA, Stephen C. Spatial epidemiology of suspected clinical leptospirosis in Sri Lanka. Epidemiol Infect 2012;140:731–743.
      doi: 10.1017/S0950268811001014pubmed: 21676347google scholar: lookup
    13. Damasco PV, Ávila CAL, Barbosa AT, Ribeiro Carvalho M, Pereira GMB, Lemos ERS, Bóia MN, Pereira MM. Atypical lymphocytosis in leptospirosis: a cohort of hospitalized cases between 1996 and 2009 in State of Rio de Janeiro, Brazil. Rev Soc Bras Med Trop 2011;44:611–615.
    14. Desvars A, Jégo S, Chiroleu F, Bourhy P, Cardinale E, Michault A. Seasonality of human leptospirosis in Reunion Island (Indian Ocean) and its association with meteorological data. Plos One 2011;6:e20377.
    15. Barcellos C, Sabroza PC. The place behind the case: leptospirosis risks and associated environmental conditions in a flood-related outbreak in Rio de Janeiro. Cad Saude Publica 2001;17:S59–S67.
      pubmed: 11426266
    16. Martins G, Penna B, Lilenbaum W. Differences between seroreactivity to leptospirosis in dairy and beef cattle from the same herd in Rio de Janeiro, Brazil. Trop Anim Health Prod 2012;44:377–378.
      doi: 10.1007/s11250-011-9918-xpubmed: 21739134google scholar: lookup
    17. Lilenbaum W, Ribeiro V, Martin E, Bispo V. Serological studies on antileptospira antibodies in Rattus norvegicus from Duque de Caxias, Rio de Janeiro, Brazil. Rev Latinoam Microbiol 1993;35:357–360.
      pubmed: 8066330
    18. Freire IMA, Varges R, Lilenbaum W. Hepatic biochemistry alterations in dogs with naturally occurring acute leptospirosis determined by Icterohaemorrhagiae serogroup. Ciênc Rural 2008;38:2630–2632.
    19. Martins G, Penna B, Lilenbaum W. Maintenance of Leptospira infection in cattle under tropical conditions. Vet Rec 2010;167:629–630.
      doi: 10.1136/vr.c5695pubmed: 21257450google scholar: lookup
    20. Otaka DY, Martins G, Hamond C, Penna B, Medeiros MA, Lilenbaum W. Serology and PCR for bovine leptospirosis: herd and individual approaches. Vet Rec 2012;170:338.
      pubmed: 22427387
    21. Lilenbaum W. Leptospirosis on animal reproduction: IV. Serological findings in mares from six farms in Rio de Janeiro, Brazil (1993–1996). Braz J Vet Res Anim Sci 1998;35:61–63.
    22. Pinna AE, Martins G, Hamond C, Lilenbaum W, Medeiros MA. Molecular diagnostics of leptospirosis in horses is becoming increasingly important. Vet Microbiol 2011;153:413.
      doi: 10.1016/j.vetmic.2011.06.015pubmed: 21763086google scholar: lookup
    23. Pinna M, Varges R, Abreu R, Lilenbaum W. Outbreak of equine leptospirosis by S. Bratislava. Online J Vet Res 2007;11:1–4.
    24. Lilenbaum W, de Souza GN, Ristow P, Moreira MC, Fráguas S, Cardoso V, Oelemann WMR. A serological study on Brucella abortus, caprine arthritis-encephalitis virus and Leptospira in dairy goats in Rio de Janeiro, Brazil. Vet J 2007;173:408–412.
      doi: 10.1016/j.tvjl.2005.12.003pubmed: 16455276google scholar: lookup
    25. Ramos ACF, Souza GN, Lilenbaum W. Influence of leptospirosis on reproductive performance of sows in Brazil. Theriogenology 2006;66:1021–1025.
    26. Lilenbaum W, Monteiro RV, Ristow P, Fraguas S, Cardoso VS, Fedullo LPL. Leptospirosis antibodies in mammals from Rio de Janeiro Zoo, Brazil. Res Vet Sci 2002;73:319–321.
      doi: 10.1016/S0034-5288(02)00099-1pubmed: 12443693google scholar: lookup
    27. Lilenbaum W, Varges R, Moraes IA, Ferreira AMR, Pissinatti A. Leptospiral antibodies in captive lion tamarins (Leontopithecus sp.) in Brazil. Vet J 2005;169:462–464.
      doi: 10.1016/j.tvjl.2004.03.015pubmed: 15848790google scholar: lookup
    28. Lilenbaum W, Monteiro RV, Albuquerque CE, Ristow P, Fraguas S, Cardoso VS, Fedullo LPL. Leptospiral antibodies in wild felines from Rio de Janeiro Zoo, Brazil. Vet J 2004;168:191–193.
      doi: 10.1016/S1090-0233(03)00139-4pubmed: 15301770google scholar: lookup
    29. OIE. Manual of Diagnostic Tests and Vaccines for Terrestrial Animals. Paris: World Organisation for Animal Health; 2008.
    30. Goris GA, Leeflang MMG, Boer K. Establishment of valid laboratory case definition for human leptospirosis. J Bacteriol Parasitol 2012;03:132.
    31. Dos Santos JP, Lima-Ribeiro AMC, Oliveira PR, dos Santos MP, Ferreira A, Medeiros AA, Tavares TCF. Seroprevalence and risk factors for leptospirosis in goats in Uberlândia, Minas Gerais, Brazil. Trop Anim Health Prod 2012;44:101–106.
      doi: 10.1007/s11250-011-9894-1pubmed: 21647773google scholar: lookup
    32. Jorge RSP, Ferreira F, Ferreira Neto JS, Vasconcellos S, Lima E, Morais ZM, Souza GO. Exposure of free-ranging wild carnivores, horses and domestic dogs to Leptospira spp in the northern Pantanal, Brazil. Mem Inst Oswaldo Cruz 2011;106:441–444.
    33. Oliveira Lavinsky M, Said RA, Strenzel GMR, Langoni H. Seroprevalence of anti-Leptospira spp. antibodies in dogs in Bahia, Brazil. Prev Vet Med 2012;106:79–84.
    34. Picardeau M. Diagnosis and epidemiology of leptospirosis. Med Mal Infect 2013;43:1–9.
      doi: 10.1016/j.medmal.2012.11.005pubmed: 23337900google scholar: lookup
    35. Levett PN. Usefulness of serologic analysis as a predictor of the infecting serovar in patients with severe leptospirosis. Clin Infect Dis 2003;36:447–452.
      doi: 10.1086/346208pubmed: 12567302google scholar: lookup
    36. Athanazio DA, Silva EF, Santos CS, Rocha GM, Vannier-Santos MA, McBride AJA, Ko AI, Reis MG. Rattus norvegicus as a model for persistent renal colonization by pathogenic Leptospira interrogans. Acta Trop 2008;105:176–180.
    37. Eshghi A, Cullen PA, Cowen L, Zuerner RL, Cameron CE. Global proteome analysis of Leptospira interrogans. J Proteome Res 2009;8:4564–4578.
      doi: 10.1021/pr9004597pmc: PMC2757032pubmed: 19663501google scholar: lookup
    38. Desvars A, Naze F, Vourc’h G, Cardinale E, Picardeau M, Michault A, Bourhy P. Similarities in Leptospira serogroup and species distribution in animals and humans in the Indian Ocean Island of Mayotte. Am J Trop Med Hyg 2012;87:134–140.
      doi: 10.4269/ajtmh.2012.12-0102pmc: PMC3391038pubmed: 22764304google scholar: lookup
    39. Faria MT, Calderwood MS, Athanazio DA, McBride AJA, Hartskeerl RA, Pereira MM, Ko AI, Reis MG. Carriage of Leptospira interrogans among domestic rats from an urban setting highly endemic for leptospirosis in Brazil. Acta Trop 2008;108:1–5.
    40. Jimenez-Coello M, Vado-Solis I, Cárdenas-Marrufo MF, Rodríguez-Buenfil JC, Ortega-Pacheco A. Serological survey of canine leptospirosis in the tropics of Yucatan Mexico using two different tests. Acta Trop 2008;106:22–26.
    41. Pinna MH, Martins G, Pinheiro ACO, Almeida DS, Oriá AP, Lilenbaum W. Detection of anti-Leptospira antibodies in captive nonhuman primates from Salvador, Brazil. Am J Primatol 2012;74:8–11.
      doi: 10.1002/ajp.21005pubmed: 22052355google scholar: lookup
    42. Lilenbaum W, Rodrigues F, Barboza F. Serological survey of leptospirosis in dogs of a city located at the Amazonian region in Brazil. Rev Bras Ciênc Vet 2000;7:133–135.
    43. Jori F, Galvez H, Mendoza P, Cespedes M, Mayor P. Monitoring of leptospirosis seroprevalence in a colony of captive collared peccaries (Tayassu tajacu) from the Peruvian Amazon. Res Vet Sci 2009;86:383–387.
      doi: 10.1016/j.rvsc.2008.09.009pubmed: 19000627google scholar: lookup
    44. Subharat S, Wilson P, Heuer C, Collins-Emerson J. Longitudinal serological survey and herd-level risk factors for Leptospira spp. serovars Hardjo-bovis and Pomona on deer farms with sheep and/or beef cattle. N Z Vet J 2012;60:215–222.
      doi: 10.1080/00480169.2012.663323pubmed: 22506918google scholar: lookup
    45. Rajeev S, Berghaus RD, Overton MW, Pence ME, Baldwin CA. Comparison of fluorescent antibody and microscopic agglutination testing for Leptospira in pregnant and nonpregnant cows. J Vet Diagn Investig 2010;22:51–54.
      doi: 10.1177/104063871002200108pubmed: 20093682google scholar: lookup
    46. Ryan EG, Leonard N, O’Grady L, Doherty ML, More SJ. Herd-level risk factors associated with Leptospira Hardjo seroprevalence in Beef/Suckler herds in the Republic of Ireland. Ir Vet J 2012;65:6.
      doi: 10.1186/2046-0481-65-6pmc: PMC3342215pubmed: 22449264google scholar: lookup
    47. De Nardi G, Genovez ME, Ribeiro MG, Castro V, Jorge AM. An in vitro growth inhibition test for measuring the potency of Leptospira spp. Sejroe group vaccine in buffaloes. Biologicals 2010;38:474–478.
    48. Ishikawa MM, Fonseca AH, Soares CO, Yoshinari NH. Comparative serological study of Lyme Borreliosis, Brucelosis and Leptospirosis in cattle. Rev Patol Trop 1999;28:195–291.
    49. Martins G, Brandão FZ, Hamond C, Medeiros M, Lilenbaum W. Diagnosis and control of an outbreak of leptospirosis in goats with reproductive failure. Vet J 2012;193:600–601.
      doi: 10.1016/j.tvjl.2012.01.016pubmed: 22365844google scholar: lookup

    Citations

    This article has been cited 28 times.
    1. Stein MA, Costa NB, da Silva GCP. Describing diagnosis (MAT + PCR) and control (vaccine + streptomycin sulfate) of a leptospirosis outbreak in dairy cows with reproductive disorders. Anim Reprod 2025;22(4):e20250011.
      doi: 10.1590/1984-3143-AR2025-0011pubmed: 41112080google scholar: lookup
    2. Pescador-Gutiérrez AM, Chávez-Sánchez JF, Galaviz-Silva L, Zarate-Ramos JJ, Villarreal-Villarreal JP, Bernal-García SE, Castillo-Velázquez U, Cervantes-Vega R, Avalos-Ramirez R. Bovine Leptospirosis: Serology, Isolation, and Risk Factors in Dairy Farms of La Laguna, Mexico. Life (Basel) 2025 Aug 2;15(8).
      doi: 10.3390/life15081224pubmed: 40868872google scholar: lookup
    3. Guzmán DA, Diaz E, Sáenz C, Álvarez H, Cueva R, Zapata-Ríos G, Prado-Vivar B, Falconí M, Pearson T, Barragan V. Domestic dogs in indigenous Amazonian communities: Key players in Leptospira cycling and transmission?. PLoS Negl Trop Dis 2024 Apr;18(4):e0011671.
      doi: 10.1371/journal.pntd.0011671pubmed: 38568912google scholar: lookup
    4. Romanowski TNA, Dias RA, Heinemann MB, Carvalho SF, Silva TA, Martins ADS, Caetano GDDC, Ferreira Júnior Á, Santos JPD, Borsanelli AC. Seroprevalence of Equine Leptospirosis in the State of Goiás, Brazil. Vet Sci 2023 Sep 25;10(10).
      doi: 10.3390/vetsci10100590pubmed: 37888542google scholar: lookup
    5. Dossou HJ, Le Guyader M, Gauthier P, Badou S, Etougbetche J, Houemenou G, Djelouadji Z, Dobigny G. Fine-scale prevalence and genetic diversity of urban small mammal-borne pathogenic Leptospira in Africa: A spatiotemporal survey within Cotonou, Benin. Zoonoses Public Health 2022 Sep;69(6):643-654.
      doi: 10.1111/zph.12953pubmed: 35524648google scholar: lookup
    6. Ospina-Pinto MC, Hernández-Rodríguez P. Identification of Leptospira spp. in the animal-environment interface (swine-water) in pig production cycle. Trop Anim Health Prod 2021 Feb 8;53(1):155.
      doi: 10.1007/s11250-021-02567-9pubmed: 33555432google scholar: lookup
    7. Abreu JAP, Krawczak FDS, Guedes IB, Souza-Filho AF, Souza GO, Binder LC, de Oliveira CS, Sponchiado J, Melo GL, Labruna MB, Heinemann MB. Frequency of anti-Leptospira spp. antibodies in dogs and wild small mammals from rural properties and conservation units in southern Brazil. One Health 2019 Dec;8:100104.
      doi: 10.1016/j.onehlt.2019.100104pubmed: 31649994google scholar: lookup
    8. Boey K, Shiokawa K, Rajeev S. Leptospira infection in rats: A literature review of global prevalence and distribution. PLoS Negl Trop Dis 2019 Aug;13(8):e0007499.
      doi: 10.1371/journal.pntd.0007499pubmed: 31398190google scholar: lookup
    9. Siqueira CC, Fraga DBM, Chagas-Junior AD, Athanazio DA, Silva MMN, Cerqueira RB, da C McBride FW, Pinna MH, Ayres MCC. Seroprevalence and risk factors associated with equineleptospirosis in the metropolitan region of Salvador and Recôncavo Baiano region, Bahia state (NE Brazil). Trop Anim Health Prod 2020 Jan;52(1):31-39.
      doi: 10.1007/s11250-019-01956-5pubmed: 31289965google scholar: lookup
    10. Almeida DS, Paz LN, de Oliveira DS, Silva DN, Ristow P, Hamond C, Costa F, Portela RW, Estrela-Lima A, Pinna MH. Investigation of chronic infection by Leptospira spp. in asymptomatic sheep slaughtered in slaughterhouse. PLoS One 2019;14(5):e0217391.
      doi: 10.1371/journal.pone.0217391pubmed: 31120961google scholar: lookup
    11. Polo N, Machado G, Rodrigues R, Nájera Hamrick P, Munoz-Zanzi C, Pereira MM, Bercini M, Timm LN, Schneider MC. A One Health Approach to Investigating Leptospira Serogroups and Their Spatial Distributions among Humans and Animals in Rio Grande do Sul, Brazil, 2013⁻2015. Trop Med Infect Dis 2019 Feb 27;4(1).
      doi: 10.3390/tropicalmed4010042pubmed: 30818803google scholar: lookup
    12. Chadsuthi S, Chalvet-Monfray K, Wiratsudakul A, Suwancharoen D, Cappelle J. A remotely sensed flooding indicator associated with cattle and buffalo leptospirosis cases in Thailand 2011-2013. BMC Infect Dis 2018 Nov 29;18(1):602.
      doi: 10.1186/s12879-018-3537-3pubmed: 30497412google scholar: lookup
    13. Libonati HA, Santos GB, Souza GN, Brandão FZ, Lilenbaum W. Leptospirosis is strongly associated to estrus repetition on cattle. Trop Anim Health Prod 2018 Oct;50(7):1625-1629.
      doi: 10.1007/s11250-018-1604-9pubmed: 29721805google scholar: lookup
    14. Pinna MH, Martins G, Loureiro AP, Lilenbaum W. Detection of bovine carriers of Leptospira by serological, bacteriological, and molecular tools. Trop Anim Health Prod 2018 Apr;50(4):883-888.
      doi: 10.1007/s11250-018-1512-zpubmed: 29349716google scholar: lookup
    15. Dezzutto D, Barbero R, Canale G, Acutis PL, Biolatti C, Dogliero A, Mitzy MD, Francone P, Colzani A, Bergagna S, Gennero MS. Detection of Leptospira spp. in Water Turtle (Trachemys scripta) Living in Ponds of Urban Parks. Vet Sci 2017 Oct 10;4(4).
      doi: 10.3390/vetsci4040051pubmed: 29056709google scholar: lookup
    16. Vieira AS, Pinto PS, Lilenbaum W. A systematic review of leptospirosis on wild animals in Latin America. Trop Anim Health Prod 2018 Feb;50(2):229-238.
      doi: 10.1007/s11250-017-1429-ypubmed: 28967042google scholar: lookup
    17. Sant'anna R, Vieira AS, Grapiglia J, Lilenbaum W. High number of asymptomatic dogs as leptospiral carriers in an endemic area indicates a serious public health concern. Epidemiol Infect 2017 Jul;145(9):1852-1854.
      doi: 10.1017/S0950268817000632pubmed: 28367783google scholar: lookup
    18. Campos ÂP, Miranda DFH, Rodrigues HWS, da Silva Carneiro Lustosa M, Martins GHC, Mineiro ALBB, Castro V, Azevedo SS, de Sousa Silva SMM. Seroprevalence and risk factors for leptospirosis in cattle, sheep, and goats at consorted rearing from the State of Piauí, northeastern Brazil. Trop Anim Health Prod 2017 Jun;49(5):899-907.
      doi: 10.1007/s11250-017-1255-2pubmed: 28357645google scholar: lookup
    19. Denipitiya DT, Chandrasekharan NV, Abeyewickreme W, Hartskeerl RA, Hapugoda MD. Identification of cattle, buffaloes and rodents as reservoir animals of Leptospira in the District of Gampaha, Sri Lanka. BMC Res Notes 2017 Mar 23;10(1):134.
      doi: 10.1186/s13104-017-2457-4pubmed: 28330498google scholar: lookup
    20. Pinto PS, Libonati H, Lilenbaum W. A systematic review of leptospirosis on dogs, pigs, and horses in Latin America. Trop Anim Health Prod 2017 Feb;49(2):231-238.
      doi: 10.1007/s11250-016-1201-8pubmed: 27909915google scholar: lookup
    21. Langoni H, Kuribara IY, Ferreira Lopes Correa AP, Ullmann LS, Sánchez GP, Lucheis SB. Anti-leptospirosis agglutinins in Brazilian capybaras (hydrochoerus hydrochaeris). J Venom Anim Toxins Incl Trop Dis 2016;22:4.
      doi: 10.1186/s40409-016-0059-6pubmed: 26819575google scholar: lookup
    22. Pinto Pda S, Libonati H, Penna B, Lilenbaum W. A systematic review on the microscopic agglutination test seroepidemiology of bovine leptospirosis in Latin America. Trop Anim Health Prod 2016 Feb;48(2):239-48.
      doi: 10.1007/s11250-015-0954-9pubmed: 26581437google scholar: lookup
    23. Jorge S, Monte LG, De Oliveira NR, Collares TF, Roloff BC, Gomes CK, Hartwig DD, Dellagostin OA, Hartleben CP. Phenotypic and Molecular Characterization of Leptospira interrogans Isolated from Canis familiaris in Southern Brazil. Curr Microbiol 2015 Oct;71(4):496-500.
      doi: 10.1007/s00284-015-0857-zpubmed: 26100241google scholar: lookup
    24. Nagalingam M, Thirumalesh SR, Kalleshamurthy T, Niharika N, Balamurugan V, Shome R, Sengupta PP, Shome BR, Prabhudas K, Rahman H. Comparative evaluation of recombinant LigB protein and heat-killed antigen-based latex agglutination test with microscopic agglutination test for diagnosis of bovine leptospirosis. Trop Anim Health Prod 2015 Oct;47(7):1329-35.
      doi: 10.1007/s11250-015-0867-7pubmed: 26065562google scholar: lookup
    25. Thomé S, Lessa-Aquino C, Ko AI, Lilenbaum W, Medeiros MA. Identification of immunodominant antigens in canine leptospirosis by Multi-Antigen Print ImmunoAssay (MAPIA). BMC Vet Res 2014 Dec 3;10:288.
      doi: 10.1186/s12917-014-0288-2pubmed: 25466383google scholar: lookup
    26. Petrakovsky J, Bianchi A, Fisun H, Nájera-Aguilar P, Pereira MM. Animal leptospirosis in Latin America and the Caribbean countries: reported outbreaks and literature review (2002-2014). Int J Environ Res Public Health 2014 Oct 16;11(10):10770-89.
      doi: 10.3390/ijerph111010770pubmed: 25325360google scholar: lookup
    27. Cortizo P, Loureiro AP, Martins G, do Rodrigues PR, Faria BP, Lilenbaum W, Deminicis BB. Risk factors to incidental leptospirosis and its role on the reproduction of ewes and goats of Espírito Santo state, Brazil. Trop Anim Health Prod 2015 Jan;47(1):231-5.
      doi: 10.1007/s11250-014-0684-4pubmed: 25274622google scholar: lookup
    28. Barbante P, Shimabukuro FH, Langoni H, Richini-Pereira VB, Lucheis SB. Leptospira spp. infection in sheep herds in southeast Brazil. J Venom Anim Toxins Incl Trop Dis 2014;20:20.
      doi: 10.1186/1678-9199-20-20pubmed: 24822059google scholar: lookup