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International wound journal2013; 12(1); 89-99; doi: 10.1111/iwj.12058

The role of polyphosphates in the sequestration of matrix metalloproteinases.

Abstract: This study outlines the potential of a novel therapeutic dressing for the management of chronic wounds. The dressing incorporates polyphosphate, a non toxic compound with a number of beneficial characteristics in terms of wound healing, in a foam matrix. The aim of this study was to identify the potential of polyphosphate incorporated in the foam dressing to sequester the activity of matrix metalloproteinases (MMPs) and proteases derived from Pseudomonas aeruginosa. Methods used included gelatin zymography and milk-casein agar plate analysis. Results have shown that this dressing is effectively capable of reducing the levels of MMP-2 and MMP-9 in both their active and latent forms using an in vitro model. The dressing also demonstrated the compound's potential in the regulation of P. aeruginosa derived proteases.
© 2013 The Authors. International Wound Journal © 2013 Medicalhelplines.com Inc and John Wiley & Sons Ltd.
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Publication Date: 2013-04-17 PubMed ID: 23590276PubMed Central: PMC7950866DOI: 10.1111/iwj.12058Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper presents a study about a new therapeutic dressing that uses polyphosphate to manage chronic wounds. It principally concentrates on how polyphosphate, when incorporated into foam dressing, can control the activity of matrix metalloproteinases and proteases derived from P. aeruginosa.

Introduction

The study focuses on a new approach to chronic wound management using a foam dressing that contains polyphosphate. Chronic wounds are a significant health issue as they fail to proceed via an orderly and timely process to produce anatomic and functional integrity. Traditional wound healing methods have often proved to be insufficient or ineffective. The main concept is how polyphosphate can manage matrix metalloproteinases, known as MMPs, which play a vital role in the wound healing process, and proteases from Pseudomonas aeruginosa.

  • Polyphosphate is a non-toxic compound known for its beneficial properties in healing wounds.
  • Matrix metalloproteinases (MMPs) are enzymes that break down tissue, assisting in the wound healing process by reorganizing the structures of damaged tissues.
  • Pseudomonas aeruginosa is a bacterium often responsible for infections in people with weakened immune systems, particularly infecting wounds, burner injuries, and contributing to chronic wound conditions.

Methodology

The research work used gelatin zymography and milk-casein agar plate analysis methodologies to explore the therapeutic dressing’s effectiveness.

  • Gelatin zymography is a potent technique to identify and quantify gelatinases (MMP-2 and MMP-9, key targets for this study).
  • Milk-casein agar is a protein-rich medium that supports the growth of many proteolytic bacteria, including Pseudomonas aeruginosa, and provides an effective way to test the presence and regulation of proteases from this bacterium.

Results and Discussions

The results indicated that the polyphosphate-foam dressing effectively reduces the MMP-2 and MMP-9 levels in both their active and latent forms.

  • This reduction was observed in an in-vitro model, suggesting that the method’s efficacy needs to be established through in-vivo testing.
  • Additionally, results also indicated that polyphosphate presented a potential advantage in the regulation of proteases derived from Pseudomonas aeruginosa, an outcome highly beneficial in treating chronic wounds, as the bacterium frequently contributes to chronic wound complications.

In conclusion, this study highlights the potential of polyphosphate in therapeutic dressings for effective chronic wound management, primarily through the regulation of MMPs and Pseudomonas aeruginosa-derived proteases. The findings present potential implications for the development of better wound management therapies. The promising in vitro results highlight the need for further in vivo testing to confirm these findings and their potential clinical applications.

Cite This Article

APA
McCarty SM, Percival SL, Clegg PD, Cochrane CA. (2013). The role of polyphosphates in the sequestration of matrix metalloproteinases. Int Wound J, 12(1), 89-99. https://doi.org/10.1111/iwj.12058

Publication

International wound journal
ISSN: 1742-481X
NlmUniqueID: 101230907
Country: England
Language: English
Volume: 12
Issue: 1
Pages: 89-99

Researcher Affiliations

McCarty, Sara M
  • Institute of Ageing and Chronic Disease, University of Liverpool, Liverpool, UK.
Percival, Steven L
    Clegg, Peter D
      Cochrane, Christine A

        MeSH Terms

        • Animals
        • Bandages
        • Dermis / drug effects
        • Dermis / enzymology
        • Dermis / pathology
        • Horses
        • Matrix Metalloproteinase 2 / metabolism
        • Matrix Metalloproteinase 9 / metabolism
        • Polyphosphates
        • Pseudomonas aeruginosa / physiology
        • Tissue Culture Techniques
        • Wound Healing / physiology
        • Wounds and Injuries / enzymology
        • Wounds and Injuries / microbiology
        • Wounds and Injuries / pathology

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        Citations

        This article has been cited 4 times.
        1. Schröder HC, Neufurth M, Zhou H, Wang S, Wang X, Müller WEG. Inorganic Polyphosphate: Coacervate Formation and Functional Significance in Nanomedical Applications. Int J Nanomedicine 2022;17:5825-5850.
          doi: 10.2147/IJN.S389819pubmed: 36474526google scholar: lookup
        2. Schröder HC, Wang X, Neufurth M, Wang S, Müller WEG. Biomimetic Polyphosphate Materials: Toward Application in Regenerative Medicine. Prog Mol Subcell Biol 2022;61:83-130.
          doi: 10.1007/978-3-031-01237-2_5pubmed: 35697938google scholar: lookup
        3. Pavlík V, Sojka M, Mazúrová M, Velebný V. Dual role of iodine, silver, chlorhexidine and octenidine as antimicrobial and antiprotease agents. PLoS One 2019;14(1):e0211055.
          doi: 10.1371/journal.pone.0211055pubmed: 30703114google scholar: lookup
        4. Caley MP, Martins VL, O'Toole EA. Metalloproteinases and Wound Healing. Adv Wound Care (New Rochelle) 2015 Apr 1;4(4):225-234.
          doi: 10.1089/wound.2014.0581pubmed: 25945285google scholar: lookup
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