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Veterinary journal (London, England : 1997)2008; 183(2); 217-218; doi: 10.1016/j.tvjl.2008.09.018

The stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests on stored horse blood.

Abstract: Increasing interest in the role of oxidative stress (OS) in equine medicine has highlighted the need to develop reliable methods to quantify it. In this study we describe the effect of refrigeration (at 4 degrees C) on the stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests carried out on 15 healthy horses. Blood samples, collected from the jugular vein, were immediately placed on ice and analysed using both the d-ROMs and BAP tests. Samples were also refrigerated at 4 degrees C and tested after 3, 7 and 24 h. The average results were similar for up to 24 h and minimal variations were found for each horse. The findings suggest that refrigeration is suitable for preserving equine blood samples for these assays and this approach will provide veterinarians with a technically simple, reliable test to measure OS under field conditions.
Copyright (c) 2008 Elsevier Ltd. All rights reserved.
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Publication Date: 2008-11-17 PubMed ID: 19010703DOI: 10.1016/j.tvjl.2008.09.018Google Scholar: Lookup
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Summary

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The research study is about an investigation conducted on the effect of refrigeration on the stability of reactive oxygen metabolites and biological antioxidant potential tests in stored horse blood. The results suggest that, with refrigeration, the blood samples are viable for such tests for up to 24 hours, providing a reliable process for assessing oxidative stress in horses.

Background and Objective

  • There has been a growing interest in understanding the role of oxidative stress (OS) in equine medicine. Oxidative stress refers to the imbalance between the generation of reactive oxygen species (free radicals) and the body’s antioxidant defense mechanisms.
  • A reliable way to quantitatively measure oxidative stress would be greatly beneficial to veterinarians and researchers. The goal was to establish whether refrigerating blood samples could maintain the stability needed for these measurements.

Methods

  • The researchers conducted tests on blood samples from 15 healthy horses.
  • They collected the blood from each horse’s jugular vein, placed it on ice, and analyzed it with reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests. These tests measure the presence and quantity of reactive oxygen metabolites and the blood’s potential to counteract their effects.
  • Simultaneously, they refrigerated the blood samples at 4 degrees Celsius and conducted the same tests after 3, 7, and 24 hours.

Results and Findings

  • The analysis showed that the average results remained similar over the 24-hour period, with only minimal variations detected for each horse.
  • This suggests that refrigeration at 4 degrees Celsius effectively preserves equine blood samples for d-ROMs and BAP assays for up to one day.

Implications

  • The study’s findings present a simple and consistent method for preservas a horse’s blood under different field conditions — making it easier for veterinarians to measure oxidative stress in horses.
  • This is highly important because finding effective ways of measuring oxidative stress can lead to better understanding, management, and treatment of various equine diseases and conditions linked to high levels of oxidative stress.

Cite This Article

APA
Celi P, Sullivan M, Evans D. (2008). The stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests on stored horse blood. Vet J, 183(2), 217-218. https://doi.org/10.1016/j.tvjl.2008.09.018

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 183
Issue: 2
Pages: 217-218

Researcher Affiliations

Celi, P
  • Faculty of Veterinary Science, The University of Sydney, PMB 3, Camden NSW, 2570, Australia. pietroc@camden.usyd.edu.au
Sullivan, M
    Evans, D

      MeSH Terms

      • Animals
      • Antioxidants / metabolism
      • Free Radicals / blood
      • Horses / blood
      • Oxidation-Reduction
      • Oxidative Stress / physiology
      • Refrigeration / veterinary
      • Specimen Handling / veterinary

      Citations

      This article has been cited 25 times.
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