FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Vet J / The stability of the reactive oxygen metabolites (d-ROMs) an...
Veterinary journal (London, England : 1997)2008; 183(2); 217-218; doi: 10.1016/j.tvjl.2008.09.018

The stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests on stored horse blood.

Abstract: Increasing interest in the role of oxidative stress (OS) in equine medicine has highlighted the need to develop reliable methods to quantify it. In this study we describe the effect of refrigeration (at 4 degrees C) on the stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests carried out on 15 healthy horses. Blood samples, collected from the jugular vein, were immediately placed on ice and analysed using both the d-ROMs and BAP tests. Samples were also refrigerated at 4 degrees C and tested after 3, 7 and 24 h. The average results were similar for up to 24 h and minimal variations were found for each horse. The findings suggest that refrigeration is suitable for preserving equine blood samples for these assays and this approach will provide veterinarians with a technically simple, reliable test to measure OS under field conditions.
Copyright (c) 2008 Elsevier Ltd. All rights reserved.
Get Full Text
Publication Date: 2008-11-17 PubMed ID: 19010703DOI: 10.1016/j.tvjl.2008.09.018Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study is about an investigation conducted on the effect of refrigeration on the stability of reactive oxygen metabolites and biological antioxidant potential tests in stored horse blood. The results suggest that, with refrigeration, the blood samples are viable for such tests for up to 24 hours, providing a reliable process for assessing oxidative stress in horses.

Background and Objective

  • There has been a growing interest in understanding the role of oxidative stress (OS) in equine medicine. Oxidative stress refers to the imbalance between the generation of reactive oxygen species (free radicals) and the body’s antioxidant defense mechanisms.
  • A reliable way to quantitatively measure oxidative stress would be greatly beneficial to veterinarians and researchers. The goal was to establish whether refrigerating blood samples could maintain the stability needed for these measurements.

Methods

  • The researchers conducted tests on blood samples from 15 healthy horses.
  • They collected the blood from each horse’s jugular vein, placed it on ice, and analyzed it with reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests. These tests measure the presence and quantity of reactive oxygen metabolites and the blood’s potential to counteract their effects.
  • Simultaneously, they refrigerated the blood samples at 4 degrees Celsius and conducted the same tests after 3, 7, and 24 hours.

Results and Findings

  • The analysis showed that the average results remained similar over the 24-hour period, with only minimal variations detected for each horse.
  • This suggests that refrigeration at 4 degrees Celsius effectively preserves equine blood samples for d-ROMs and BAP assays for up to one day.

Implications

  • The study’s findings present a simple and consistent method for preservas a horse’s blood under different field conditions — making it easier for veterinarians to measure oxidative stress in horses.
  • This is highly important because finding effective ways of measuring oxidative stress can lead to better understanding, management, and treatment of various equine diseases and conditions linked to high levels of oxidative stress.

Cite This Article

APA
Celi P, Sullivan M, Evans D. (2008). The stability of the reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests on stored horse blood. Vet J, 183(2), 217-218. https://doi.org/10.1016/j.tvjl.2008.09.018

Publication

Veterinary journal (London, England : 1997)
ISSN: 1532-2971
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 183
Issue: 2
Pages: 217-218

Researcher Affiliations

Celi, P
  • Faculty of Veterinary Science, The University of Sydney, PMB 3, Camden NSW, 2570, Australia. pietroc@camden.usyd.edu.au
Sullivan, M
    Evans, D

      MeSH Terms

      • Animals
      • Antioxidants / metabolism
      • Free Radicals / blood
      • Horses / blood
      • Oxidation-Reduction
      • Oxidative Stress / physiology
      • Refrigeration / veterinary
      • Specimen Handling / veterinary

      Citations

      This article has been cited 25 times.
      1. Taylor SD, Serpa PBS, Santos AP, Hart KA, Vaughn SA, Moore GE, Mukhopadhyay A, Page AE. Effects of intravenous administration of peripheral blood-derived mesenchymal stromal cells after infusion of lipopolysaccharide in horses. J Vet Intern Med 2022 Jul;36(4):1491-1501.
        doi: 10.1111/jvim.16447pubmed: 35698909google scholar: lookup
      2. Al-Khaldi K, Yimer N, Sadiq MB, Firdaus Jesse Bin Abdullah F, Salam Babji A, Al-Bulushi S. Edible bird's nest supplementation in chilled and cryopreserved Arabian stallion semen. Saudi J Biol Sci 2022 Mar;29(3):1577-1584.
        doi: 10.1016/j.sjbs.2021.11.009pubmed: 35280569google scholar: lookup
      3. Sgorbini M, Bonelli F, Percacini G, Pasquini A, Rota A. Maternal and Neonatal Evaluation of Derived Reactive Oxygen Metabolites and Biological Antioxidant Potential in Donkey Mares and Foals. Animals (Basel) 2021 Oct 3;11(10).
        doi: 10.3390/ani11102885pubmed: 34679906google scholar: lookup
      4. Hoppel F, Calabria E, Pesta DH, Kantner-Rumplmair W, Gnaiger E, Burtscher M. Effects of Ultramarathon Running on Mitochondrial Function of Platelets and Oxidative Stress Parameters: A Pilot Study. Front Physiol 2021;12:632664.
        doi: 10.3389/fphys.2021.632664pubmed: 33679442google scholar: lookup
      5. Sato S, Namisaki T, Furukawa M, Saikawa S, Kawaratani H, Kaji K, Takaya H, Shimozato N, Sawada Y, Kitagawa K, Moriya K, Akahane T, Mitoro A, Hoki N, Ann T, Yoshiji H. Effect of L-carnitine on health-related quality of life in patients with liver cirrhosis. Biomed Rep 2020 Dec;13(6):65.
        doi: 10.3892/br.2020.1372pubmed: 33149909google scholar: lookup
      6. Choi YA, Lee DH, Cho DY, Lee YJ. Outcomes Assessment of Sustainable and Innovatively Simple Lifestyle Modification at the Workplace - Drinking Electrolyzed-Reduced Water (OASIS-ERW): A Randomized, Double-Blind, Placebo-Controlled Trial. Antioxidants (Basel) 2020 Jun 27;9(7).
        doi: 10.3390/antiox9070564pubmed: 32605142google scholar: lookup
      7. Dawood MAO, Koshio S, Zaineldin AI, Van Doan H, Ahmed HA, Elsabagh M, Abdel-Daim MM. An evaluation of dietary selenium nanoparticles for red sea bream (Pagrus major) aquaculture: growth, tissue bioaccumulation, and antioxidative responses. Environ Sci Pollut Res Int 2019 Oct;26(30):30876-30884.
        doi: 10.1007/s11356-019-06223-6pubmed: 31446600google scholar: lookup
      8. Tsuzuki N, Kanbayashi Y, Kusano K. Markers for oxidative stress in the synovial fluid of Thoroughbred horses with carpal bone fracture. J Equine Sci 2019 Mar;30(1):13-16.
        doi: 10.1294/jes.30.13pubmed: 30944542google scholar: lookup
      9. Langille E, Lemieux V, Garant D, Bergeron P. Development of small blood volume assays for the measurement of oxidative stress markers in mammals. PLoS One 2018;13(12):e0209802.
        doi: 10.1371/journal.pone.0209802pubmed: 30589904google scholar: lookup
      10. Hossain MS, Koshio S, Ishikawa M, Yokoyama S, Sony NM, Islam MJ. Fishmeal replacement by soya protein concentrate with inosine monophosphate supplementation influences growth, digestibility, immunity, blood health, and stress resistance of red sea bream, Pagrus major. Fish Physiol Biochem 2019 Apr;45(2):613-629.
        doi: 10.1007/s10695-018-0581-2pubmed: 30367428google scholar: lookup
      11. Brkljača Bottegaro N, Gotić J, Šuran J, Brozić D, Klobučar K, Bojanić K, Vrbanac Z. Effect of prolonged submaximal exercise on serum oxidative stress biomarkers (d-ROMs, MDA, BAP) and oxidative stress index in endurance horses. BMC Vet Res 2018 Jul 6;14(1):216.
        doi: 10.1186/s12917-018-1540-ypubmed: 29980209google scholar: lookup
      12. Hossain MS, Koshio S. Dietary substitution of fishmeal by alternative protein with guanosine monophosphate supplementation influences growth, digestibility, blood chemistry profile, immunity, and stress resistance of red sea bream, Pagrus major. Fish Physiol Biochem 2017 Dec;43(6):1629-1644.
        doi: 10.1007/s10695-017-0398-4pubmed: 28681205google scholar: lookup
      13. Kusano K, Yamazaki M, Kiuchi M, Kaneko K, Koyama K. Reference range of blood biomarkers for oxidative stress in Thoroughbred racehorses (2-5 years old). J Equine Sci 2016;27(3):125-129.
        doi: 10.1294/jes.27.125pubmed: 27703408google scholar: lookup
      14. Tsuzuki N, Sasaki N, Kusano K, Endo Y, Torisu S. Oxidative stress markers in Thoroughbred horses after castration surgery under inhalation anesthesia. J Equine Sci 2016;27(2):77-9.
        doi: 10.1294/jes.27.77pubmed: 27330401google scholar: lookup
      15. Tsuzuki N, Endo Y, Kikkawa L, Korosue K, Kaneko Y, Kitauchi A, Katamoto H, Hidaka Y, Hagio M, Torisu S. Effects of ozonated autohemotherapy on the antioxidant capacity of Thoroughbred horses. J Vet Med Sci 2016 Jan;77(12):1647-50.
        doi: 10.1292/jvms.15-0225pubmed: 26166812google scholar: lookup
      16. Dawood MA, Koshio S, Ishikawa M, Yokoyama S. Effects of partial substitution of fish meal by soybean meal with or without heat-killed Lactobacillus plantarum (LP20) on growth performance, digestibility, and immune response of amberjack, Seriola dumerili juveniles. Biomed Res Int 2015;2015:514196.
        doi: 10.1155/2015/514196pubmed: 25705667google scholar: lookup
      17. Schneeberger K, Czirják GÁ, Voigt CC. Frugivory is associated with low measures of plasma oxidative stress and high antioxidant concentration in free-ranging bats. Naturwissenschaften 2014 Apr;101(4):285-90.
        doi: 10.1007/s00114-014-1155-5pubmed: 24515336google scholar: lookup
      18. Kapadia VS, Chalak LF, Sparks JE, Allen JR, Savani RC, Wyckoff MH. Resuscitation of preterm neonates with limited versus high oxygen strategy. Pediatrics 2013 Dec;132(6):e1488-96.
        doi: 10.1542/peds.2013-0978pubmed: 24218465google scholar: lookup
      19. Rubin M, Artusi I, Cozza G. Mapping the oxidative landscape in cystic fibrosis: methodological frontiers and application. Front Pharmacol 2025;16:1632924.
        doi: 10.3389/fphar.2025.1632924pubmed: 40740993google scholar: lookup
      20. Cocco R, Arrigo F, Sechi S, Rizzo M, Piccione G, Arfuso F. Oxidant/Antioxidant Equilibrium and Neurotransmitter Levels in Camelids Used for Circus Activities: A Preliminary Study. Vet Sci 2025 Jun 10;12(6).
        doi: 10.3390/vetsci12060570pubmed: 40559807google scholar: lookup
      21. Yuba T, Koyama Y, Takahashi A, Fujino Y, Shimada S. Association between oxidative stress and postoperative delirium in joint replacement using diacron-reactive oxygen metabolites and biological antioxidant potential tests. Sci Rep 2024 Dec 2;14(1):29854.
        doi: 10.1038/s41598-024-80739-xpubmed: 39617794google scholar: lookup
      22. Yuba T, Koyama Y, Ootaki C, Fujino Y, Shimada S. Effect of blood sample storage period on d-ROMs and BAP test data. Heliyon 2024 Jul 30;10(14):e34573.
        doi: 10.1016/j.heliyon.2024.e34573pubmed: 39113980google scholar: lookup
      23. Yuba T, Koyama Y, Kinishi Y, Uokawa R, Ootaki C, Shimada S, Fujino Y. Analysis of Maternal and Fetal Oxidative Stress During Delivery with Epidural Analgesia. Reprod Sci 2024 Sep;31(9):2753-2762.
        doi: 10.1007/s43032-024-01580-1pubmed: 38727999google scholar: lookup
      24. Taylor SD, Hart KA, Vaughn S, Giancola SC, Serpa PBS, Santos AP. Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses. J Vet Intern Med 2024 Jan-Feb;38(1):460-468.
        doi: 10.1111/jvim.16934pubmed: 37948618google scholar: lookup
      25. Tsuzuki N, Maruko T, Takeyama A, Ikeda H, Mizuguchi Y. Evaluation of oxidative stress in foals with Rhodococcus equi infection-induced pneumonia for the judgment of therapeutic effect. J Vet Med Sci 2023 Dec 6;85(12):1277-1280.
        doi: 10.1292/jvms.23-0260pubmed: 37853622google scholar: lookup
      Subscribe to our newsletter
      Join 99,359 horse owners like you who receive our email updates on horse health and nutrition.