The Temporal Associations of B-Mode and Power-Doppler Ultrasonography, and Ovarian Steroid Changes of the Periovulatory Follicle and Corpus Luteum During Luteogenesis and Luteolysis in Jennies.
Abstract: This study aimed to determine the associations between B-mode and Power-doppler ultrasonography and ovarian steroids of the periovulatory follicle and respective corpus luteum (CL) during luteogenesis and luteolysis in jennies. Twenty-four periovulatory follicles/estrus of correspondent one inter-ovulatory interval (n = 12 jennies) were assessed in the study. B-mode ultrasonography and teasing were carried out once day until the detection of a periovulatory follicle (≥28 mm, uterine edema, and signs of estrus). Thereafter, jennies were monitored at 4-hour-intervals by B-mode and Power-doppler ultrasonography. Closer to ovulation, jennies were hourly checked. Each CL was checked daily from luteogenesis to luteolysis. Plasma concentrations of estradiol and progesterone were assessed daily with chemiluminescence immunoassay. Granulosa echogenicity and thickness increased from -36 hour to -1 hour before ovulation in 70% of follicles (P < .05) and were strongly associated with impending ovulation (r = 0.80 and r = 0.70, respectively). The follicular-wall blood flow increased from -72 to -24 hour pre-ovulation, while the estradiol concentration declined from 42 pg/mL by -72 hour to 31.6 pg/mL by 24 hour before ovulation (P < .05). The vascularization of the periovulatory follicle decreased from 62% (-36 hour) to 37% (-1 hour) before ovulation (P < .05). The CL vascularization and progesterone concentration gradually increased, reaching the peak at 11- and 10-day after the ovulation, respectively (P < .05). The CL vascularization started to decline 3 day before luteolysis, while progesterone concentrations started to drop 4 day before luteolysis (P < .05). In conclusion, the structural changes of the periovulatory follicle detected on B-mode and Power-doppler can be used to detect impending ovulation in donkeys; however, Power-doppler, but not B-mode ultrasonography, can be used to assess CL function in jennies.
Copyright © 2023 Elsevier Inc. All rights reserved.
Publication Date: 2023-01-18 PubMed ID: 36669627DOI: 10.1016/j.jevs.2023.104224Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research investigates the correlations between B-mode and Power-doppler ultrasonography and ovarian steroids’ changes in the periovulatory follicle and the corpus luteum during luteogenesis and luteolysis in female donkeys. It shows that structural changes in the periovulatory follicle, detected through ultrasonography, can be used to predict impending ovulation in donkeys.
Study Details
- The study monitored 24 periovulatory follicles/estrus of corresponding one inter-ovulatory interval in 12 jennies or female donkeys.
- B-mode ultrasonography and teasing methods were deployed to detect a periovulatory follicle, which is the ovarian follicle from which the ovum or egg is released during ovulation.
- From there, the jennies were examined at four-hour intervals by B-mode and Power-doppler ultrasonography, and hourly checks were conducted closer to ovulation.
Data Collection and Key Findings
- For each Corpus Luteum (CL) – the remnant of the follicle after ovulation, checks were carried out daily from luteogenesis (formation of the corpus luteum) to luteolysis (regression of the corpus luteum).
- The concentrations of estradiol and progesterone, two crucial hormones for reproductive functions, were tested daily with a chemiluminescence immunoassay.
- The echogenicity and the thickness of the granulosa, which is a layer of cells surrounding ovum inside the follicle, showed an increase upto an hour before ovulation in 70% of the follicles.
- The blood flow in the follicular-wall saw an escalation 72 to 24 hours before ovulation, even as the estradiol concentration declined.
- Importantly, changes in the vascularization (blood supply level) of the periovulatory follicle and the CL and changes in hormone concentrations were found to be strongly associated with ovulation and luteal activity.
Conclusion
- The study concluded that the structural changes in the periovulatory follicle, as detected on B-mode and Power-doppler ultrasonography, can effectively predict impending ovulation in donkeys.
- It also indicated that Power-doppler, but not B-mode ultrasonography, can be used to evaluate CL function in jennies.
Cite This Article
APA
Magalhaes HB, Canisso IF, Dell-Aqua JA.
(2023).
The Temporal Associations of B-Mode and Power-Doppler Ultrasonography, and Ovarian Steroid Changes of the Periovulatory Follicle and Corpus Luteum During Luteogenesis and Luteolysis in Jennies.
J Equine Vet Sci, 122, 104224.
https://doi.org/10.1016/j.jevs.2023.104224 Publication
Researcher Affiliations
- Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil; Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana- Champaign, Urbana IL.
- Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil; Department of Veterinary Clinical Medicine, College of Veterinary Medicine, University of Illinois Urbana- Champaign, Urbana IL. Electronic address: canisso@illinois.edu.
- Department of Veterinary Surgery and Animal Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
MeSH Terms
- Female
- Animals
- Luteolysis / physiology
- Progesterone
- Equidae
- Corpus Luteum
- Ultrasonography
- Estradiol
- Ultrasonography, Doppler
Citations
This article has been cited 2 times.- Wang Y, Gao Z, Zhang Q, Guo X, Xia W, Gu X, Zeng W. Unraveling the Transcriptomic Profiles of Large and Small Donkey Follicles. Genes (Basel) 2025 May 20;16(5).
- Aboelmaaty AM, Elgharieb AEA, El-Debaky HA, Alkhadrawy JMH, Abou-Ahmed MM, Ghallab AM. Fluctuations of antimüllerian hormone, ovarian follicular reserve, and antioxidant status throughout the estrous cycle in aged mares. Open Vet J 2024 Nov;14(11):3132-3143.
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