The time-course of lipid biosynthesis in horse skin.
Abstract: To observe the time-course of formation of sebaceous lipids in the horse, skin was pulse-labelled in vivo by intradermal injection of [1-14C]acetate and the injection sites were harvested at intervals for up to 12 days by skin punch biopsy. The distribution of radioactivity among the major neutral lipid classes and the phospholipids from these biopsies showed that, soon after pulse-labelling, the phospholipids were highly labelled followed by a long-term decrease in radioactivity. Over the same period, the low initial labelling of the dominant component, the equolides (giant ring omega-lactones, C32-C36), was followed by a long-term increase in radioactivity. This suggests a post-pulse transferance of radioactivity from the phospholipids to the equolides, presumably in the fatty acids. Of the phospholipid fatty acids from horse dermis, including sebaceous glands, 33% were found to contain iso-branched structures unique to horse sebaceous lipids. Of the iso-branched fatty acids, 40% were delta 9-18:1 and delta 9- and delta 11-20:1 acids, which are structurally appropriate to be precursors for the monounsaturated equolides. These findings strengthen the hypothesis that the sebaceous phospholipids of horse skin serve as long-term lipid intermediates in the biosynthesis of the equolides during sebaceous cell development.
Publication Date: 1985-10-02 PubMed ID: 4041473DOI: 10.1016/0005-2760(85)90134-1Google Scholar: Lookup
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- Journal Article
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research article investigates the process and timeline of lipid biosynthesis, specifically sebaceous lipids, in horse skin. It uses radiolabeled materials to trace the transfer of materials between phospholipids and a special group of lipids found in horses, known as equolides.
Research Methodology
- Researchers used a technique called pulse-labelling to monitor the formation of sebaceous lipids in the skin of horses. This involved injecting an intradermal dose of a radiolabeled compound ([1-14C]acetate) into the horse’s skin.
- The injection sites were periodically harvested via skin punch biopsy over a span of up to 12 days.
- The distribution of radioactivity among the major neutral lipid classes and the phospholipids was then observed and analyzed.
Results and Findings
- Soon after pulse-labelling, it was noticed that phospholipids demonstrated high levels of radioactivity, which gradually decreased over time.
- Simultaneously, the equolides, a dominant lipid component produced by horse skin, displayed low initial labelling (indicating lower initial absorption of the radiolabeled compound) but exhibited a long-term increase in radioactivity.
- This shift in radioactivity suggests a post-pulse transfer of radioactivity from the phospholipids to the equolides, likely through fatty acids.
Unique Properties of Horse Lipids
- Among the phospholipid fatty acids found in horse dermis, including sebaceous glands, around 33% contained iso-branched structures. These are unique to horse sebaceous lipids and are not typically seen in other species.
- Of these unique iso-branched fatty acids, 40% were classified as delta 9-18:1 and delta 9- and delta 11-20:1 acids. Structurally, these acids are potential precursors for the monounsaturated equolides.
Conclusion
- The findings indicate that the phospholipids in horse skin act as long-term lipid intermediates in the biosynthesis of the equolides, and this happens during the development of the sebaceous cell.
- The research strengthens the hypothesis that the sebaceous phospholipids of horse skin play a major role in the formation of equolides.
Cite This Article
APA
Colton SW, Downing DT.
(1985).
The time-course of lipid biosynthesis in horse skin.
Biochim Biophys Acta, 836(3), 306-311.
https://doi.org/10.1016/0005-2760(85)90134-1 Publication
Researcher Affiliations
MeSH Terms
- Acetates / metabolism
- Animals
- Biopsy
- Carbon Radioisotopes
- Horses
- Kinetics
- Lipids / biosynthesis
- Skin / cytology
- Skin / metabolism
- Time Factors
Grant Funding
- AM22083 / NIADDK NIH HHS
Citations
This article has been cited 1 times.- Pannkuk EL, McGuire LP, Gilmore DF, Savary BJ, Risch TS. Glycerophospholipid analysis of Eastern red bat (Lasiurus borealis) hair by electrospray ionization tandem mass spectrometry.. J Chem Ecol 2014 Mar;40(3):227-35.
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