[The use of amikacin in the treatment of endometritis caused by Pseudomonas aeruginosa in mares].

The research article investigates the use of amikacin sulphate for treating endometritis in mares caused by the bacteria Pseudomonas aeruginosa.

Methodology

• The study involved six mares from which Pseudomonas aeruginosa was isolated from endometrial biopsies.
• Different methods of administering amikacin sulphate were applied in the cases. These methods include intra-uterine application, intramuscular injection, and intravenous route.
• Amikacin was given via the intra-uterine route in three of the mares, one mare received an intramuscular injection, another was given the drug intravenously, and in the last mare, both local and intravenous treatments were used simultaneously.
• An intra-uterine Tris-EDTA instillation was also performed before the uterine amikacin instillations to assist in the breakdown of the capsule around the bacterium.
• Serum concentrations of amikacin were monitored after both intravenous and intramuscular administrations.

Findings

• The highest concentration of 15 microgram/ml was reached one hour after intravenous injection, after which it fell below the therapeutic level.
• To maintain an effective concentration, the drug needed to be administered every 6-8 hours.
• All mares treated via the intra-uterine or intravenous routes recovered from endometritis.

Conclusion

• The study reemphasized the importance of obtaining cultures from uterine biopsies for accurate diagnosis as Pseudomonas was cultured only once from the conventional cervical swab, while the other five mares showed negative results in cervical swabs.