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Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells.
Abstract: Wound healing after skin injury is a complex process, particularly in equines where leg wounds are prevalent and their repair is complicated due to the anatomical characteristics. Conventional treatments are not effective enough. The umbilical cord offers an unlimited source of adult mesenchymal stem cells (ucMSCs) from Wharton's jelly tissue. The present study aims to demonstrate the safety and therapeutic potential of the allogeneic use of equine ucMSCs (e-ucMSCs) in the healing of severe equine leg wounds. The methods employed were the isolation, culture and expansion of e-ucMSCs. Flow cytometry and a PCR assay were used for cell characterization. This study included an immunomodulation assay, a murine pre-clinical trial and the first phase of an equine clinical trial. Our results showed that e-ucMSCs express a functional HLA-G homolog, EQMHCB2. In the immunomodulation assay, the e-ucMSCs inhibited the proliferation of activated equine peripheral blood mononuclear cells (e-PBMCs). In the murine pre-clinical trial, e-ucMSCs reduced healing time by 50%. In the equine clinical trial, the injection of e-ucMSCs into severe leg lesions improved the closure time and quality of the tissues involved, regenerating them without fibrous tissue scar formation. In conclusion, the results of this study suggest that e-ucMSCs can be used allogeneically for wound healing by creating a tolerogenic environment.
Publication Date: 2024-02-16 PubMed ID: 38397024PubMed Central: PMC10889822DOI: 10.3390/ijms25042350Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research article outlines an experiment which studies the potential of using equine umbilical cord stem cells (e-ucMSCs) in healing severe leg injuries in horses. The study found that these stem cells can indeed promote quicker and higher quality wound healing, suggesting their possible future use in medical applications.
Objective and Methodology
- The research seeks to find more effective treatments for wound healing in horses, particularly those injuries on the leg which can be complex due to the animal’s specific anatomy.
- The team hypothesizes that mesenchymal stem cells (MSCs), derived from the Wharton’s jelly tissue of the equine umbilical cord, could potentially help in wound recovery.
- The methodology employed for the study includes the isolation, culture, and proliferation of the umbilical cord stem cells. The team also performed characterization assays on the cells through flow cytometry and a Polymerase Chain Reaction (PCR) assay.
Immunomodulation Assay, Preclinical Trial and Clinical Trial
- An immunomodulation assay was conducted to test how the stem cells interact with the immune cells of the horses. It was found that e-ucMSCs inhibited the proliferation of activated horse immune cells, creating a more tolerogenic or accepting environment for recovery.
- A preclinical trial was conducted on mice where the use of stem cells resulted in a reduction of healing time by around 50%, showcasing the stem cells’ potential efficacy.
- The first phase of a clinical trial on horses was also conducted. The stem cells were injected into severe leg lesions where it was observed that the healing time of the wounds was improved and the quality of tissue regeneration also enhanced, with no fibrous scar tissue formation.
Conclusion
- The researchers concluded that e-ucMSCs could be used to improve wound healing in horses, creating a tolerogenic or accepting environment for recovery. This suggests a promising future in the use of e-ucMSCs for wound healing applications in equine medical settings.
Cite This Article
APA
Iribarne A, Palma MB, Andrini L, Riccillo F, Rodriguez D, Casella M, Garay F, Zabala JS, Mazza L, Muro A, Buero G, Miriuka SG, Carosella E, Garcu00eda MN.
(2024).
Therapeutic Potential in Wound Healing of Allogeneic Use of Equine Umbilical Cord Mesenchymal Stem Cells.
Int J Mol Sci, 25(4), 2350.
https://doi.org/10.3390/ijms25042350 Publication
Researcher Affiliations
- Cu00e1tedra de Citologu00eda, Histologu00eda y Embriologu00eda, Facultad de Ciencias Mu00e9dicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
- Cu00e1tedra de Citologu00eda, Histologu00eda y Embriologu00eda, Facultad de Ciencias Mu00e9dicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
- Instituto de Neurociencias (INEU), Fundaciu00f3n para la Lucha contra Enfermedades Neurolu00f3gicas de la Infancia-Consejo Nacional de Investigaciones Cientu00edficas y Tu00e9cnicas (FLENI-CONICET) Escobar, Buenos Aires CP B1625, Argentina.
- Cu00e1tedra de Citologu00eda, Histologu00eda y Embriologu00eda, Facultad de Ciencias Mu00e9dicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
- Cu00e1tedra de Citologu00eda, Histologu00eda y Embriologu00eda, Facultad de Ciencias Mu00e9dicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
- Centro de Reproducciu00f3n Equina La Adela, Chascomu00fas, Buenos Aires CP 7130, Argentina.
- Divisiu00f3n Remonta Haras La Teruca, Buenos Aires B1900, Argentina.
- Divisiu00f3n Remonta Haras La Teruca, Buenos Aires B1900, Argentina.
- Direcciu00f3n Veterinaria, Policu00eda de la Provincia de Buenos Aires, Buenos Aires B1904, Argentina.
- Divisiu00f3n Remonta Haras La Teruca, Buenos Aires B1900, Argentina.
- Direcciu00f3n Veterinaria, Policu00eda de la Provincia de Buenos Aires, Buenos Aires B1904, Argentina.
- Cu00e1tedra de Anatomu00eda B, Facultad de Ciencias Mu00e9dicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
- Laboratorio de Anu00e1lisis Clu00ednicos Veterinarios, La Plata, Buenos Aires CP 1900, Argentina.
- Sanatorio Mater Dei, Ciudad Autu00f3noma de Buenos Aires, Buenos Aires CP C1425, Argentina.
- Instituto de Neurociencias (INEU), Fundaciu00f3n para la Lucha contra Enfermedades Neurolu00f3gicas de la Infancia-Consejo Nacional de Investigaciones Cientu00edficas y Tu00e9cnicas (FLENI-CONICET) Escobar, Buenos Aires CP B1625, Argentina.
- Commissariat a l'Energie Atomique et aux Energies Alternatives (CEA), Service de Recherche en Hemato-Immunologie (SRHI), Saint-Louis Hospital, 75010 Paris, France.
- Hopital Saint-Louis, IUH, Universitu00e9 Paris Diderot, Sorbonne Paris Cite, 75010 Paris, France.
- Cu00e1tedra de Citologu00eda, Histologu00eda y Embriologu00eda, Facultad de Ciencias Mu00e9dicas, Universidad Nacional de La Plata, Buenos Aires CP 1900, Argentina.
MeSH Terms
- Animals
- Horses
- Mice
- Leukocytes, Mononuclear
- Mesenchymal Stem Cells
- Umbilical Cord
- Cicatrix
- Hematopoietic Stem Cell Transplantation
Conflict of Interest Statement
The authors declare no conflicts of interest.
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