Tissue and plasma activity of lactic dehydrogenase and creatine kinase in the horse.

This study investigates the activity of lactic dehydrogenase and creatine kinase enzymes in various tissues of a horse, and reveals their concentration differences in skeletal muscle, cardiac muscle, kidney, and liver.

Lactic Dehydrogenase Distribution

• Lactic dehydrogenase, also known as LDH, is distributed widely in various tissues of the horse.
• This enzyme was found in higher concentrations mainly in skeletal muscle, cardiac muscle, kidney, and liver tissues.
• The patterns of isoenzymes, which are enzymes that differ in amino acid sequence but catalyze the same chemical reaction, showed a selective concentration of LDH5 in skeletal muscle.
• On the other hand, LDH1 and LDH2 were predominantly present in cardiac tissues of the horse.

Creatine Kinase Distribution

• The study also inspected the distribution of another enzyme, creatine kinase.
• Contrary to lactic dehydrogenase, creatine kinase was found primarily in skeletal and cardiac muscle tissues.
• This enzyme did not show a significant presence in other tissues as compared to LDH.

Enzyme Concentration in Serum

• The research further studied the concentration of these enzymes in the serum, which is the clear, pale-yellow liquid that remains from blood plasma after clotting factors have been removed by clot formation.
• Both enzymes exhibited a significant range of activity in the horse’s serum, indicating their involvement in various metabolic activities in the body.

This study provides valuable insights into the tissue-specific activity and distribution of lactic dehydrogenase and creatine kinase in the horse, paving the way for deeper understanding and potential diagnostic utility of these enzymes in equine health and disease.