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Home / Equine Research Bank / Animals (Basel) / Towards the Identification of New Biomarkers in Saliva and S...
Animals : an open access journal from MDPI2024; 14(21); doi: 10.3390/ani14213105

Towards the Identification of New Biomarkers in Saliva and Serum for Treatment Monitoring of Equine Gastric Ulcer Syndrome: A Liquid Proteomic Approach.

Abstract: Equine gastric ulcer syndrome (EGUS) is a common condition in horses. This study explores the use of liquid proteomics to identify new biomarkers in saliva and serum to monitor EGUS treatment. The proteomes of horses with EGUS before and after a successful treatment with omeprazole were analysed. In saliva, 503 proteins were identified, with 7 upregulated and 6 downregulated post-treatment. Among the proteins that changed, there was an increase in vimentin, linked to wound healing, and a decrease in podocalyxin, associated with tissue damage. In serum, 206 proteins were found, with significant changes in 5. Keratin type I increased, supporting epithelial integrity, whereas immunoglobulin lambda decreased, indicating a reduced immune response. Gene ontology analysis revealed a decrease in immune-related pathways after successful treatment. Overall, 13 proteins in saliva and 5 in serum showed significant changes after treatment, highlighting the differential responses of saliva and serum in EGUS. This report creates new avenues for discovering potential biomarkers to monitor EGUS treatment, which is of high importance for the management of this prevalent disease.
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Publication Date: 2024-10-28 PubMed ID: 39518828PubMed Central: PMC11545413DOI: 10.3390/ani14213105Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates new biomarkers in horse saliva and serum for monitoring the treatment of Equine Gastric Ulcer Syndrome (EGUS) using a liquid proteomic approach. The study identifies significant changes in certain proteins in both saliva and serum after treatment, providing useful insights for potential future EGUS treatment monitoring.

Explanation of Research

  • The researchers focused on EGUS, a prevalent condition in horses. Their approach involved monitoring EGUS treatment via new biomarkers discovered in saliva and serum through a technique called liquid proteomics.
  • Liquid proteomics is a process that explores protein substance within a liquid sample, such as serum or saliva. Using this approach, the team hoped to identify new biomarkers relatively unique or distinctive biological indicators that can be used to monitor the development and treatment of the disease.
  • The study used horses with EGUS and analysed their proteomes, the entire set of their proteins, before and after successful treatment with omeprazole, a medication commonly used for EGUS treatment.

Saliva and Serum Analysis

  • From the saliva samples, 503 proteins were identified. Of these, 7 were found to be upregulated (their expression levels increased) and 6 were downregulated (their expression levels decreased) after treatment.
  • In particular, the protein vimentin, which has been linked to wound healing, showed an increase. Conversely, podocalyxin, which is typically associated with tissue damage, decreased.
  • From the serum samples, 206 proteins were identified with significant changes observed in 5 of these after treatment. Keratin type I, a protein that supports the integrity of the epithelium, showed an increase. The immunoglobulin lambda, associated with the immune response, showed a decrease. These changes indicate a shift in the biological environment within the horse that is correlated with successful treatment of EGUS.
  • Furthermore, a gene ontology analysis, a study of how genes behave, revealed a decrease in immune-related pathways following effective treatment. This suggests that the horse’s immune response changes significantly after successful treatment.

Implications for Management of EGUS

  • Overall, the study found that 13 proteins in saliva and 5 in serum showed significant changes following treatment. This is important because it shows that saliva and serum can have different responses to EGUS, highlighting the need for differential treatment strategies.
  • The identification of potential biomarkers could help veterinarians and researchers better monitor the treatment of EGUS, a common equine condition.
  • By revealing how proteins behave in relation to EGUS and its treatment, this study contributes valuable knowledge to the current understanding of the disease and offers potential strategies for its successful management.

Cite This Article

APA
Muñoz-Prieto A, Rubić I, Rešetar Maslov D, González-Sánchez JC, Mrljak V, Cerón JJ, Hansen S. (2024). Towards the Identification of New Biomarkers in Saliva and Serum for Treatment Monitoring of Equine Gastric Ulcer Syndrome: A Liquid Proteomic Approach. Animals (Basel), 14(21). https://doi.org/10.3390/ani14213105

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 14
Issue: 21

Researcher Affiliations

Muñoz-Prieto, Alberto
  • Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain.
Rubić, Ivana
  • Laboratory of Proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
Rešetar Maslov, Dina
  • Laboratory of Proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
González-Sánchez, Juan Carlos
  • BioQuant, Heidelberg University, Im Neuenheimer Feld 267, 69120 Heidelberg, Germany.
Mrljak, Vladimir
  • Laboratory of Proteomics, Internal Diseases Clinic, Faculty of Veterinary Medicine, University of Zagreb, 10000 Zagreb, Croatia.
Cerón, Jose Joaquín
  • Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Regional Campus of International Excellence 'Campus Mare Nostrum', University of Murcia, Campus de Espinardo s/n, 30100 Murcia, Spain.
Hansen, Sanni
  • Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark.

Grant Funding

  • RYC2021-033660-I / Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación (AEI), Spain, and The Euro-pean Next Generation Funds (NextgenerationEU)

Conflict of Interest Statement

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

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