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BMC genetics2020; 21(1); 46; doi: 10.1186/s12863-020-00843-5

Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse.

Abstract: Horse testis development and spermatogenesis are complex physiological processes. To study these processes, three immature and three mature testes were collected from the Mongolian horse, and six libraries were established using high-throughput RNA sequencing technology (RNA-Seq) to screen for genes related to testis development and spermatogenesis. A total of 16,237 upregulated genes and 8,641 downregulated genes were detected in the testis of the Mongolian horse. These genes play important roles in different developmental stages of spermatogenesis and testicular development. Five genes with alternative splicing events that may influence spermatogenesis and development of the testis were detected. GO (Gene ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses were performed for functional annotation of the differentially expressed genes. Pathways related to "spermatogenesis," male gamete generation," "spermatid development" and "oocyte meiosis" were significantly involved in different stages of testis development and spermatogenesis. Genes, pathways and alternative splicing events were identified with inferred functions in the process of spermatogenesis in the Mongolian horse. The identification of these differentially expressed genetic signatures improves our understanding of horse testis development and spermatogenesis.
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Publication Date: 2020-04-28 PubMed ID: 32345215PubMed Central: PMC7187496DOI: 10.1186/s12863-020-00843-5Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research on the Mongolian horse has studied the development of the testis and the process of spermatogenesis, using high-throughput RNA sequencing to identify relevant genes. It has helped to improve our understanding of these processes by revealing upregulated and downregulated genes, as well as pathways and alternative splicing events.

Research Approach

  • The researchers studied testis development and spermatogenesis in the Mongolian horse by obtaining samples from three immature and three mature testes.
  • They used high-throughput RNA sequencing technology (RNA-Seq) to create six separate libraries for further analysis.

Findings

  • The research detected a total of 16,237 upregulated genes and 8,641 downregulated genes in the horse’s testis.
  • These genes are believed to have significant roles at different stages of testis development and spermatogenesis.
  • Five genes were found to have alternative splicing events that could influence the development of the testis and spermatogenesis.

Analysis

  • After identifying the differentially expressed genes, the researchers performed GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses for functional annotation.
  • These analyses revealed that pathways related to “spermatogenesis,” “male gamete generation,” “spermatid development” and “oocyte meiosis” were significantly involved in the process of testis development and spermatogenesis.

Conclusions

  • The study successfully identified genes and pathways alongside alternative splicing events with inferred functions in the process of spermatogenesis in the Mongolian horse.
  • These findings improve our understanding of the physiological processes underlying horse testis development and spermatogenesis, providing a valuable resource for further research in this field.

Cite This Article

APA
Li B, He X, Zhao Y, Bai D, Du M, Song L, Liu Z, Yin Z, Manglai D. (2020). Transcriptome profiling of developing testes and spermatogenesis in the Mongolian horse. BMC Genet, 21(1), 46. https://doi.org/10.1186/s12863-020-00843-5

Publication

BMC genetics
ISSN: 1471-2156
NlmUniqueID: 100966978
Country: England
Language: English
Volume: 21
Issue: 1
Pages: 46

Researcher Affiliations

Li, Bei
  • College of Animal Science, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • lnner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
He, Xiaolong
  • Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, 010031, China.
Zhao, Yiping
  • College of Animal Science, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • lnner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Bai, Dongyi
  • College of Animal Science, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • lnner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Du, Ming
  • College of Animal Science, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • lnner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Song, Lianjie
  • College of Animal Science, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • lnner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Liu, Zhuang
  • College of Animal Science, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • lnner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Yin, Zhenchen
  • College of Animal Science, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • lnner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
  • Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China.
Manglai, Dugarjaviin
  • College of Animal Science, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China. dmanglai@163.com.
  • lnner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China. dmanglai@163.com.
  • Scientific Observing and Experimental Station of Equine Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Equine Research Center, Inner Mongolia Agricultural University, Hohhot, 010018, China. dmanglai@163.com.

MeSH Terms

  • Alternative Splicing
  • Animals
  • High-Throughput Nucleotide Sequencing
  • Horses / genetics
  • Male
  • Mongolia
  • Spermatogenesis / genetics
  • Testis / growth & development
  • Transcriptome

Grant Funding

  • 2019ZD03 / Natural Science Foundation of Inner Mongolia

Conflict of Interest Statement

The authors declare that they have no competing interests.

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