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Home / Equine Research Bank / Sci Rep / Transcriptomic profiling of mare endometrium at different st...
Scientific reports2023; 13(1); 16263; doi: 10.1038/s41598-023-43359-5

Transcriptomic profiling of mare endometrium at different stages of endometrosis.

Abstract: In the current study, transcriptome profiles of mare endometrium, classified into categories I, IIA, and IIB according to Kenney and Doig, were compared using RNA sequencing, analyzed, and functionally annotated using in silico analysis. In the mild stage (IIA) of endometrosis compared to category I endometrium, differentially expressed genes (DEGs) were annotated to inflammation, abnormal metabolism, wound healing, and quantity of connective tissue. In the moderate stage (IIB) of endometrosis compared to category I endometrium, DEGs were annotated to inflammation, fibrosis, cellular homeostasis, mitochondrial dysfunction, and pregnancy disorders. Ingenuity pathway analysis (IPA) identified cytokines such as transforming growth factor (TGF)-β1, interleukin (IL)-4, IL-13, and IL-17 as upstream regulators of DEGs associated with cellular homeostasis, metabolism, and fibrosis signaling pathways. In vitro studies showed the effect of these cytokines on DEGs such as ADAMTS1, -4, -5, -9, and HK2 in endometrial fibroblasts at different stages of endometrosis. The effect of cytokines on ADAMTS members' gene transcription in fibroblasts differs according to the severity of endometrosis. The identified transcriptomic changes associated with endometrosis suggest that inflammation and metabolic changes are features of mild and moderate stages of endometrosis. The changes of ADAMTS-1, -4, -5, -9, in fibrotic endometrium as well as in endometrial fibroblast in response to TGF-β1, IL-4, IL-13, and IL-17 suggest the important role of these factors in the development of endometrosis.
© 2023. The Author(s).
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Publication Date: 2023-09-27 PubMed ID: 37758834PubMed Central: PMC10533846DOI: 10.1038/s41598-023-43359-5Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on the detailed analysis of gene expression in the endometrium of mares at different stages of endometrosis through RNA sequencing and computer-based analysis. The study identifies specific gene changes related to inflammation, metabolism, fibrosis, and cellular function in association with the disease severity, suggesting potential therapeutic targets.

Research Design and Methodology

  • The researchers undertook transcriptome profiling of the mare endometrium at different stages of endometrosis. Endometrosis was categorized into stages I, IIA, and IIB according to Kenney and Doig’s system.
  • RNA sequencing was used to compare the transcriptomes of endometrial stages. This sequencing allows for the understanding of genetic information and its associated processes such as differentiation and metabolism.
  • The data from RNA sequencing was analyzed and functionally annotated using in silico (computer-based) analysis. This helped the researchers understand the role of different genes and their functions within each category of endometrosis.

Key Findings

  • The genes that were differently expressed at a mild stage of endometrosis (IIA), compared to the non-endometrosis stage (I), were associated with inflammation, abnormal metabolism, wound healing, and connective tissue quantity.
  • At a moderate stage of endometrosis (IIB), the differently expressed genes were linked with inflammation, fibrosis, cellular homeostasis, mitochondrial dysfunction, and pregnancy disorders.
  • The researchers identified certain cytokines (signaling proteins that mediate and regulate immunity, inflammation and hematopoiesis) such as Transforming Growth Factor (TGF)-β1, Interleukin (IL)-4, IL-13, and IL-17, that act as upstream regulators of these gene changes. These cytokines were found to influence the signaling pathways related to cellular homeostasis, metabolism, and fibrosis.
  • In vitro observational studies showed the effect of these cytokines on genes like ADAMTS1, -4, -5, -9, and HK2 in fibroblasts (the most common cells of connective tissue in animals) at different stages of endometrosis.
  • The transcription of ADAMTS genes in fibroblasts was found to be affected differently by the cytokines, depending on the severity of endometrosis.

Conclusion

  • This research indicates that inflammation and metabolic changes are key features in mild and moderate stages of endometrosis which is primarily manifested by the gene changes.
  • The changes observed in the fibroblasts’ ADAMTS genes and the endometrium, particularly the impact of TGF-β1, IL-4, IL-13, and IL-17, suggest a vital role of these factors in the development of endometrosis.

This study helps to shed more light on the genetic and molecular mechanisms that could be contributing to the development and progression of endometrosis in mares, and presents potential therapeutic targets for this disease.

Cite This Article

APA
Szóstek-Mioduchowska A, Wójtowicz A, Sadowska A, Moza Jalali B, Słyszewska M, Łukasik K, Gurgul A, Szmatoła T, Bugno-Poniewierska M, Ferreira-Dias G, Skarzynski DJ. (2023). Transcriptomic profiling of mare endometrium at different stages of endometrosis. Sci Rep, 13(1), 16263. https://doi.org/10.1038/s41598-023-43359-5

Publication

Scientific reports
ISSN: 2045-2322
NlmUniqueID: 101563288
Country: England
Language: English
Volume: 13
Issue: 1
Pages: 16263

Researcher Affiliations

Szóstek-Mioduchowska, A
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences in Olsztyn, Olsztyn, Poland. a.szostek-mioduchowska@pan.olsztyn.pl.
Wójtowicz, A
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences in Olsztyn, Olsztyn, Poland.
Sadowska, A
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences in Olsztyn, Olsztyn, Poland.
Moza Jalali, B
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences in Olsztyn, Olsztyn, Poland.
Słyszewska, M
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences in Olsztyn, Olsztyn, Poland.
Łukasik, K
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences in Olsztyn, Olsztyn, Poland.
Gurgul, A
  • Center for Experimental and Innovative Medicine, University of Agriculture in Cracow, Cracow, Poland.
Szmatoła, T
  • Center for Experimental and Innovative Medicine, University of Agriculture in Cracow, Cracow, Poland.
Bugno-Poniewierska, M
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Cracow, Cracow, Poland.
Ferreira-Dias, G
  • Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
Skarzynski, D J
  • Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research Polish Academy of Sciences in Olsztyn, Olsztyn, Poland.

MeSH Terms

  • Pregnancy
  • Animals
  • Female
  • Horses
  • Transcriptome
  • Interleukin-13
  • Interleukin-17
  • Cytokines / genetics
  • Endometrium
  • Inflammation / genetics
  • Fibrosis

Conflict of Interest Statement

The authors declare no competing interests.

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