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Transmission ratio distortion detection by neutral genetic markers in the Pura Raza Española horse breed.
Abstract: Transmission Ratio Distortion (TRD) is a genetic phenomenon widely demonstrated in several livestock species, but barely in equine species. The TRD occurs when certain genotypes are over- or under-represented in the offspring of a particular mating and can be caused by a variety of factors during gamete formation or during embryonic development. For this study, 126 394 trios consisting of a stallion, mare, and offspring were genotyped using a panel of 17 neutral microsatellite markers recommended by the International Society for Animal Genetics for paternity tests and individual identification. The number of alleles available for each marker ranges from 13 to 18, been 268 the total number of alleles investigated. The TRDscan v.2.0 software was used with the biallelic procedure to identify regions with distorted segregation ratios. After completing the analysis, a total of 12 alleles (out of 11 microsatellites) were identified with decisive evidence for genotypic TRD; 3 and 9 with additive and heterosis patterns, respectively. In addition, 19 alleles (out of 10 microsatellites) were identified displaying allelic TRD. Among them, 14 and 5 were parent-unspecific and stallion-mare-specific TRD. Out of the TRD regions, 24 genes were identified and annotated, predominantly associated with cholesterol metabolism and homeostasis. These genes are often linked to non-specific symptoms like impaired fertility, stunted growth, and compromised overall health. The results suggest a significant impact on the inheritance of certain genetic traits in horses. Further analysis and validation are needed to better understand the TRD impact before the potential implementation in the horse breeding programme strategies.
Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.
Publication Date: 2023-10-12 PubMed ID: 37950978DOI: 10.1016/j.animal.2023.101012Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The research investigates a genetic anomaly known as Transmission Ratio Distortion (TRD) in the Pura Raza Española horse breed. The study identifies specific regions of TRD using neutral genetic markers and proposes further understanding of its impact on horse breeding programs.
Research Methodology
- The researchers studied TRD in a huge sample size of 126,394 trios consisting of a stallion, mare, and their offspring. Each trio was genotyped using a panel of 17 neutral microsatellite markers as recommended by the International Society for Animal Genetics for paternity tests and individual identification.
- The neutral genetic markers used in the study had a varying number of alleles, ranging from 13 to 18, and the total number of alleles investigated was 268.
- The TRDscan v.2.0 software was employed to identify regions with distorted segregation ratios, thus pointing out potential areas of TRD.
Findings of the Study
- The study successfully identified a total of 12 decisive alleles (out of 11 microsatellites) for genotypic TRD. Out of these, 3 and 9 followed additive and heterosis patterns respectively.
- A further 19 alleles (out of 10 microsatellites) were determined to display allelic TRD. 14 of these were parent-unspecific while 5 were specifically associated with the stallion-mare.
- The analysis also identified and annotated 24 genes within the TRD regions which were mostly associated with cholesterol metabolism and homeostasis. These annotated genes could potentially be linked to various non-specific health issues like impaired fertility, stunted growth, and compromised overall health.
Implications and Further Research
- The study suggests that TRD can have a significant impact on the inheritance of certain genetic traits in horses, specifically in the Pura Raza Española breed.
- These findings may potentially alter horse breeding strategies once a better understanding of the TRD and its impact is attained.
- However, the researchers concede that further analysis and validation are needed before these results can be effectively utilized in horse breeding programme strategies.
Cite This Article
APA
Perdomo-Gonzu00e1lez DI, Id-Lahoucine S, Molina A, Cu00e1novas A, Laseca N, Azor PJ, Valera M.
(2023).
Transmission ratio distortion detection by neutral genetic markers in the Pura Raza Española horse breed.
Animal, 17(11), 101012.
https://doi.org/10.1016/j.animal.2023.101012 Publication
Researcher Affiliations
- Departamento de Agronomu00eda, ETSIA, Universidad de Sevilla, Sevilla 41005, Spain. Electronic address: dperdomo@us.es.
- Department of Animal and Veterinary Science, Scotland's Rural College, Easter Bush, Edinburgh EH25 9RG, United Kingdom.
- Departamento de Genu00e9tica, Universidad de Cu00f3rdoba, Cu00f3rdoba 14014, Spain.
- Center of Genetic Improvement of Livestock, Department of Animal Biosciences, University of Guelph, Guelph, ON N1G 2W1, Canada.
- Departamento de Genu00e9tica, Universidad de Cu00f3rdoba, Cu00f3rdoba 14014, Spain.
- Real Asociaciu00f3n Nacional de Criadores de Caballos de Pura Raza Espau00f1ola (ANCCE), Sevilla 41014, Spain.
- Departamento de Agronomu00eda, ETSIA, Universidad de Sevilla, Sevilla 41005, Spain.
MeSH Terms
- Horses / genetics
- Animals
- Male
- Female
- Genetic Markers
- Genotype
- Phenotype
- Inheritance Patterns
- Software
- Alleles
Citations
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