Urine cartilage oligomeric matrix protein (COMP) measurement is useful in discriminating the osteoarthritic Thoroughbreds.
Abstract: To quantify the urinary concentration of cartilage oligomeric matrix protein (COMP), and to evaluate the relationship between urinary COMP concentration and the catabolic activity of synovial fluid (SF) in diseased horses. Methods: COMP in horse urine was detected by immunoblotting with a monoclonal antibody (mAb; 14G4) raised against equine COMP from articular cartilage. Urine and serum samples were obtained from 83 Thoroughbred horses with aseptic joint diseases (AJD, 79 horses) or septic joint diseases (SJD, four horses) at the time of anesthesia induction, and samples of SF were obtained during surgery. Control samples of urine (n=111) were collected from normal horses free of any orthopedic diseases after they had been racing. COMP concentration was determined in all samples using inhibition enzyme-linked immunosorbent assay (ELISA) with mAb 14G4. SF samples were also used for the quantification of gelatinase activity. Results: Positive bands of COMP fragments were determined on the immunoblots with mAb 14G4. The urinary COMP concentrations in AJD and SJD horses (1.02+/-0.75 and 1.55+/-1.17 microg/100mg creatinine, respectively) were significantly higher than normal (0.57+/-0.29 microg/100mg creatinine). In 55 horses with fractures in the AJD group there was a logarithmic relationship (r=-0.45, P<0.001) between the urinary and SF COMP measurements, while the urinary COMP level was positively correlated with matrix metalloproteinase (MMP)-2 and -9 activities (r=0.30, P<0.05 and r=0.51, P<0.001, respectively) in SF. Conclusions: The urinary COMP assay with mAb 14G4 is useful for discriminating horses with osteoarthritis. The higher COMP levels in urine from such horses would be indicative of enhanced proteolytic activity, in addition to the increased COMP levels in the diseased joints.
Publication Date: 2006-08-08 PubMed ID: 16895759DOI: 10.1016/j.joca.2006.04.017Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Articular Cartilage
- Biochemistry
- Cartilage
- Clinical Study
- Diagnosis
- Disease Diagnosis
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Equine Diseases
- Extracellular matrix
- Horses
- Immunoblotting
- In Vivo
- Joint Health
- Monoclonal Antibodies
- Osteoarthritis
- Proteoglycans
- Synovial Fluid
- Thoroughbreds
- Urine Analysis
- Veterinary Medicine
- Veterinary Research
Summary
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This study investigates the concentration of cartilage oligomeric matrix protein (COMP) in horse urine to determine its usefulness in identifying osteoarthritis in Thoroughbred horses. It shows that the concentration of urinary COMP is significantly higher in horses with specific joint diseases, indicating an increased proteolytic activity, which can serve as a marker for osteoarthritis diagnosis.
Research Methodology
- The study involved a total of 83 Thoroughbred horses with aseptic joint diseases (AJD, 79 horses), or septic joint diseases (SJD, four horses). Additionally, 111 control samples were collected from healthy horses with no history of orthopedic diseases after a race.
- COMP in horse urine was detected using a method called immunoblotting, which involved using a monoclonal antibody raised against equine COMP from articular cartilage.
- The concentration of COMP in the urine and serum samples was determined using an inhibition enzyme-linked immunosorbent assay (ELISA) procedure. Samples of synovial fluid (SF) were obtained from the horses during surgery and evaluated for gelatinase activity.
- The study compared urinary COMP concentrations among AJD and SJD horses and the control group.
Study Findings
- The researchers found that urinary COMP concentrations in horses with AJD and SJD were significantly higher than those in the control group. Mean concentrations were 1.02 and 1.55 micrograms/100mg creatinine for AJD and SJD horses, respectively, compared to 0.57 micrograms/100mg creatinine in healthy horses.
- In particular, 55 horses with fractures in the AJD group showed a logarithmic relationship between urinary and SF COMP measurements, and the urinary COMP level positively correlated with matrix metalloproteinase (MMP)-2 and -9 activities in the SF, which are enzymes involved in the breakdown of extracellular matrix in normal physiological processes and disease processes.
Conclusion
- The study concludes that an assay of urinary COMP, using a monoclonal antibody, can help in distinguishing horses with osteoarthritis. The elevated urinary COMP levels indicate enhanced proteolytic activity that is usually related to articular cartilage degradation and can thus assist in diagnosing disease conditions.
Cite This Article
APA
Misumi K, Tagami M, Kamimura T, Miyakoshi D, Helal IE, Arai K, Fujiki M.
(2006).
Urine cartilage oligomeric matrix protein (COMP) measurement is useful in discriminating the osteoarthritic Thoroughbreds.
Osteoarthritis Cartilage, 14(11), 1174-1180.
https://doi.org/10.1016/j.joca.2006.04.017 Publication
Researcher Affiliations
- Department of Veterinary Clinical Science, Kagoshima University, Kagoshima, Japan. kmisumi@agri.kagoshima-u.ac.jp
MeSH Terms
- Animals
- Cartilage, Articular / metabolism
- Creatinine / urine
- Enzyme-Linked Immunosorbent Assay / methods
- Extracellular Matrix Proteins / blood
- Extracellular Matrix Proteins / urine
- Female
- Fractures, Bone / metabolism
- Fractures, Bone / urine
- Fractures, Bone / veterinary
- Glycoproteins / blood
- Glycoproteins / urine
- Horse Diseases / metabolism
- Horse Diseases / urine
- Horses
- Immunoblotting / methods
- Joint Diseases / metabolism
- Joint Diseases / urine
- Joint Diseases / veterinary
- Male
- Matrilin Proteins
- Osteoarthritis / diagnosis
- Osteoarthritis / urine
- Osteoarthritis / veterinary
- Sepsis / metabolism
- Sepsis / urine
- Sepsis / veterinary
- Synovial Fluid / metabolism
Citations
This article has been cited 4 times.- Smith R, Önnerfjord P, Holmgren K, di Grado S, Dudhia J. Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease. Int J Mol Sci 2020 Mar 20;21(6).
- Riegger J, Rehm M, Büchele G, Brenner H, Günther KP, Rothenbacher D, Brenner RE. Serum Cartilage Oligomeric Matrix Protein in Late-Stage Osteoarthritis: Association with Clinical Features, Renal Function, and Cardiovascular Biomarkers. J Clin Med 2020 Jan 18;9(1).
- Ekman S, Lindahl A, Rüetschi U, Jansson A, Björkman K, Abrahamsson-Aurell K, Björnsdóttir S, Löfgren M, Hultén LM, Skiöldebrand E. Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (COMP) neo-epitope in horses. Equine Vet J 2019 Sep;51(5):674-680.
- Clutterbuck AL, Smith JR, Allaway D, Harris P, Liddell S, Mobasheri A. High throughput proteomic analysis of the secretome in an explant model of articular cartilage inflammation. J Proteomics 2011 May 1;74(5):704-15.
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