Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. I. Erythrocytes, platelets, and total leukocyte counts.
Abstract: The Sysmex XT-2000iV is a laser-based, flow cytometric hematology system that has been introduced for use in large and referral veterinary laboratories. Objective: The purpose of this study was to validate the Sysmex XT-2000iV for counting erythrocytes, reticulocytes, platelets, and total leukocytes in blood from ill dogs, cats, and horses. Methods: Blood samples from diseased animals (133 dogs, 65 cats, and 73 horses) were analyzed with the Sysmex XT-2000iV and the CELL-DYN 3500. Manual reticulocyte counts were done on an additional 98 canine and 14 feline samples and manual platelet counts were done on an additional 73 feline and 55 canine samples, and compared with automated Sysmex results. Results: Hemoglobin concentration, RBC counts, and total WBC counts on the Sysmex were highly correlated with those from the CELL-DYN (r>/=0.98). Systematic differences occurred for MCV and HCT. MCHC was poorly correlated in all species (r=0.33-0.67). The Sysmex impedance platelet count in dogs was highly correlated with both the impedance count from the CELL-DYN (r=0.99) and the optical platelet count from the Sysmex (r=0.98). The Sysmex optical platelet count included large platelets, such that in samples from cats, the results agreed better with manual platelet counts than with impedance platelet counts on the Sysmex. Canine reticulocyte counts on the Sysmex correlated well (r=0.90) with manual reticulocyte counts. Feline reticulocyte counts on the Sysmex correlated well with aggregate (r=0.86) but not punctate (r=0.50) reticulocyte counts. Conclusions: The Sysmex XT-2000iV performed as well as the CELL-DYN on blood samples from dogs, cats, and horses with a variety of hematologic abnormalities. In addition, the Sysmex detected large platelets and provided accurate reticulocyte counts.
Publication Date: 2009-04-06 PubMed ID: 19392763DOI: 10.1111/j.1939-165X.2009.00125.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study examined the efficacy of the Sysmex XT-2000iV, a hematology system, in counting different types of blood cells in sick dogs, cats, and horses. The research found that Sysmex XT-2000iV was as effective as the current CELL-DYN 3500 system and also efficiently detected large platelets and counted reticulocytes accurately.
Objectives and Methodology
- The primary focus was to validate the utility and accuracy of the Sysmex XT-2000iV hematology system for veterinary purposes, specifically for counting erythrocytes, reticulocytes, platelets, and total leukocytes in the blood of ill dogs, cats, and horses.
- The study used blood samples from 133 dogs, 65 cats, and 73 horses, all of which were diseased. These samples were analyzed using both the Sysmex XT-2000iV and the currently used CELL-DYN 3500 systems for comparison.
- Manual reticulocyte and platelet counts were also conducted on additional feline and canine samples for further comparison with the automated Sysmex results.
Results
- The results showed a high correlation (r>/=0.98) between counts of hemoglobin concentration, RBC (Red Blood Cells), and total WBC (White Blood Cells) on the Sysmex system and the CELL-DYN system.
- However, systematic differences were observed for MCV (Mean Corpuscular Volume) and HCT (Hematocrit).
- MCHC (Mean Corpuscular Hemoglobin Concentration) correlation was lower in all species, ranging from 0.33-0.67.
- The Sysmex impedance platelet count in dogs showed a strong correlation with both the impedance count from the CELL-DYN (r=0.99) and Sysmex’s optical platelet count (r=0.98).
- The Sysmex system showed the ability to detect large platelets effectively. In cat samples, results aligned better with manual platelet counts than with impedance platelet counts on Sysmex.
- Reticulocyte counts using the Sysmex system for dogs and cats also showed a good correlation with manual counts.
Conclusion
- The study concluded that the Sysmex XT-2000iV system performs just as well as the CELL-DYN system on blood samples from dogs, cats, and horses with various hematologic abnormalities.
- In addition, the Sysmex system effectively detected large platelets and provided accurate counts of reticulocytes, a feature the traditional manuals counts or other systems might miss.
Cite This Article
APA
Lilliehöök I, Tvedten H.
(2009).
Validation of the Sysmex XT-2000iV hematology system for dogs, cats, and horses. I. Erythrocytes, platelets, and total leukocyte counts.
Vet Clin Pathol, 38(2), 163-174.
https://doi.org/10.1111/j.1939-165X.2009.00125.x Publication
Researcher Affiliations
- University Animal Hospital, Swedish University of Agricultural Sciences, Uppsala, Sweden. inger.lilliehook@uds.slu.se
MeSH Terms
- Animals
- Blood Cell Count / instrumentation
- Blood Cell Count / veterinary
- Blood Platelets / cytology
- Cats / blood
- Dogs / blood
- Erythrocytes / cytology
- Horses / blood
- Leukocytes / cytology
- Reproducibility of Results
- Sensitivity and Specificity
- Species Specificity
Citations
This article has been cited 10 times.- Betting A, Schweighauser A, Francey T. Diagnostic value of reticulocyte indices for the assessment of the iron status of cats with chronic kidney disease.. J Vet Intern Med 2022 Mar;36(2):619-628.
- Grebert M, Granat F, Braun JP, Leroy Q, Bourgès-Abella N, Trumel C. Validation of the Sysmex XN-V hematology analyzer for canine specimens.. Vet Clin Pathol 2021 Jun;50(2):184-197.
- Dondi F, Vasylyeva K, Serafini F, Gruarin M, Troìa R, Giunti M, Agnoli C. Heinz body-related interference with leukocyte and erythrocyte variables obtained by an automated hematology analyzer in cats.. J Vet Diagn Invest 2019 Sep;31(5):704-713.
- Riond B, Waßmuth AK, Hartnack S, Hofmann-Lehmann R, Lutz H. Study on the kinetics and influence of feline platelet aggregation and deaggregation.. BMC Vet Res 2015 Nov 5;11:276.
- Brandeker E, Hillström A, Hanås S, Hagman R, Holst BS. The effect of a single dose of prednisolone in dogs envenomated by Vipera berus--a randomized, double-blind, placebo-controlled clinical trial.. BMC Vet Res 2015 Feb 26;11:44.
- Rossi G, Cerquetella M, Antonelli E, Pengo G, Magi GE, Villanacci V, Rostami-Nejad M, Spaterna A, Bassotti G. The importance of histologic parameters of lacteal involvement in cases of canine lymphoplasmacytic enteritis.. Gastroenterol Hepatol Bed Bench 2015 Winter;8(1):33-41.
- Stirn M, Moritz A, Bauer N. Rate of manual leukocyte differentials in dog, cat and horse blood samples using ADVIA 120 cytograms.. BMC Vet Res 2014 Jun 5;10:125.
- Fujino Y, Nakamura Y, Matsumoto H, Fukushima K, Takahashi M, Ohno K, Tsujimoto H. Development and evaluation of a novel in-clinic automated hematology analyzer, ProCyte Dx, for canine erythrocyte indices, leukogram, platelet counts and reticulocyte counts.. J Vet Med Sci 2013 Nov;75(11):1519-24.
- Rossi G, Capitani L, Ceciliani F, Restelli L, Paltrinieri S. Hyposialylated α1-acid glycoprotein inhibits phagocytosis of feline neutrophils.. Res Vet Sci 2013 Oct;95(2):465-71.
- Shmukler BE, Rivera A, Vandorpe DH, Alves J, Bonfanti U, Paltrinieri S, Alper SL. Cation-leak stomatocytosis in standard schnauzers does not cosegregate with coding mutations in the RhAG, SLC4A1, or GLUT1 genes associated with human disease.. Blood Cells Mol Dis 2012 Apr 15;48(4):219-25.
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