Variation in expression of MHC class II antigens on horse lymphocytes determined by MHC haplotype.
Abstract: A panel of monoclonal antibodies was used to characterize the expression of equine Major Histocompatibility Complex (MHC) class II antigens on lymphocytes of horses of different MHC types. MHC class II antigen expression was compared between adult horses and foals, and the level of expression of MHC class II antigens on horse T cell subpopulations was also determined. Peripheral blood lymphocytes (PBL) from young and adult healthy horses of different MHC haplotypes were labeled with the antibodies and assayed by single- and two-color immunofluorescence flow cytometry. A variation in the expression of equine MHC class II antigens on PBL was identified that was determined by MHC haplotype. Both young and old animals carrying the MHC class II haplotype D3 expressed lower levels of MHC class II antigens on their PBL than did horses of other MHC class II types. D3 heterozygotes had an intermediate level of expression. Neonates and foals of any MHC haplotype had lower levels of expression of MHC class II antigens than did adults. MHC class II antigens were identified on surface immunoglobulin positive (sIg+) B cells as well as on T cells, with the B cells staining brightest. Dual staining for MHC class II antigens and markers for equine T cell subsets demonstrated that both EqCD4+ and EqCD8+ T cells expressed MHC class II molecules. These results suggest that the relatively low levels of MHC class II antigen expression on foal and neonate lymphocytes may be developmentally regulated, and not determined by environmental factors. The cause of the low expression of MHC class II antigens linked to the MHC class II haplotype D3 is not known, but it appears to reflect a decreased expression by all lymphocyte subpopulations.
Publication Date: 1994-07-01 PubMed ID: 7975179DOI: 10.1016/0165-2427(94)90093-0Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
- Research Support
- U.S. Gov't
- P.H.S.
Summary
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The research aims to establish the relationship between the expression of Major Histocompatibility Complex (MHC) class II antigens on horse lymphocytes and the MHC haplotype. It finds that the level of MHC class II expression is lower in both neonate and young animals and those carrying the MHC class II D3, regardless of their age.
Methodology
- The researchers used monoclonal antibodies to uncover the expression of MHC class II antigens in horses with varying MHC types.
- They examined the antigen expression in both adult and young horses, and various T cell subsets.
- They took peripheral blood lymphocytes (PBL) from healthy horses and labeled them with antibodies.
- The labeled PBL were then analyzed using single and two-color immunofluorescence flow cytometry, which helps measure cell characteristics.
Findings
- They established a variation in MHC class II antigen expression among the PBL, which appeared to be influenced by the MHC haplotype.
- Both young and older horses carrying the MHC class II D3 had a lower level of MHC class II antigen expression in comparison to horses with different MHC class II types.
- MHC class II antigens were found on both B cells and T cells, with B cells showing the greatest staining.
- Neonate and foal horses exhibited a lower level of MHC class II antigen expression, regardless of their MHC haplotype.
- Both EqCD4+ and EqCD8+ T cells expressed MHC class II molecules, as identified by dual staining of antigens and cell markers.
Conclusion
- The results suggest that the lower level of MHC class II antigen expression in neonate and young horses is likely developmentally regulated, rather than influenced by environmental factors.
- The research does not provide a reason for the low MHC class II antigen expression linked with the MHC class II D3, but speculates it may be due to decreased expression across all lymphocyte subgroups.
Cite This Article
APA
Barbis DP, Bainbridge D, Crump AL, Zhang CH, Antczak DF.
(1994).
Variation in expression of MHC class II antigens on horse lymphocytes determined by MHC haplotype.
Vet Immunol Immunopathol, 42(1), 103-114.
https://doi.org/10.1016/0165-2427(94)90093-0 Publication
Researcher Affiliations
- Equine Genetics Center, James A. Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853.
MeSH Terms
- Animals
- Antibodies, Monoclonal
- B-Lymphocytes / immunology
- Female
- Flow Cytometry / veterinary
- Genetic Variation
- Haplotypes / immunology
- Histocompatibility Antigens Class II / genetics
- Histocompatibility Antigens Class II / immunology
- Horses / immunology
- Major Histocompatibility Complex / genetics
- Major Histocompatibility Complex / immunology
- Male
- T-Lymphocytes / immunology
Citations
This article has been cited 1 times.- Raza F, Babasyan S, Larson EM, Freer HS, Schnabel CL, Wagner B. Peripheral blood basophils are the main source for early interleukin-4 secretion upon in vitro stimulation with Culicoides allergen in allergic horses. PLoS One 2021;16(5):e0252243.
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