Whole-Blood Validation of a New Point-of-care Equine Serum Amyloid A Assay.
Abstract: Serum amyloid A (SAA) is considered a major acute phase protein (APP) in horses. Serum amyloid A stall-side assays are commercially available to assess the inflammatory response of patients with various infectious and noninfectious conditions. The objective of this study was to determine the analytical performance of a new point-of-care (POC) assay for the measurement of SAA in whole blood and plasma of horses. One hundred and sixty blood samples were collected from 60 horses at various time points after immunization with an equine core vaccine. Analytical validation of the SAA POC assay included the measurement of SAA in whole blood and plasma, assessment of linearity and precision, and comparison of the SAA POC results with those obtained with a validated turbidimetric immunoassay (TIA). The SAA POC assay yielded similar results in whole blood and plasma (P > .05), and the results were positively correlated with the TIA (R2 = 0.964). The assay displayed solid linearity throughout the detection range of ≤ 20 to 3,000 μg/mL (R2 = 0.984) with inter-assay and intra-assay coefficients of variation ranging from 7.8% to 13.3% and 5.7% to 12.0%, respectively. The new SAA POC assay was able to reliably measure SAA in both whole blood and plasma. Similar to previously validated assays, the new SAA POC assay is a valuable tool to investigate the inflammatory response in various clinical diseases of horses.
Copyright © 2020 The Authors. Published by Elsevier Inc. All rights reserved.
Publication Date: 2020-08-15 PubMed ID: 33077080DOI: 10.1016/j.jevs.2020.103222Google Scholar: Lookup
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- Journal Article
Summary
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This research evaluated the performance of a new point-of-care assay for measuring Serum Amyloid A in the blood and plasma of horses, finding it to be a reliable tool for understanding the inflammatory response in various clinical diseases.
Introduction
- The aim of this study was to determine the performance of a new point-of-care (POC) assay that is used to measure Serum Amyloid A (SAA), a major acute phase protein in horses. This protein is usually assessed using stall-side assays to evaluate the inflammatory response in horses suffering from different infectious or noninfectious conditions.
Methodology
- A total of 160 blood samples were collected from 60 horses after they were immunized with an equine core vaccine. The collection of samples happened at different time points to allow for diverse data.
- To validate the performance of the SAA POC assay, the study performed several tasks. Firstly, it assessed SAA in both whole blood and plasma. It also evaluated the linearity between the blood and plasma results and its precision. Lastly, the SAA POC results were compared with an already validated turbidimetric immunoassay (TIA).
Results
- The SAA POC assay showed similar results in whole blood and plasma, thereby confirming its effectiveness for use in both mediums.
- Also, a positive correlation was found between the POC assay and the validated TIA. This gives even more validation to the SAA POC assay since TIA is a trusted method.
- The assay demonstrated strong linearity within the detection range of ≤ 20 to 3,000 μg/mL.
- The inter-assay and intra-assay coefficients of variation ranged from 7.8% to 13.3% and 5.7% to 12.0% respectively, which signifies that the assay is not only reliable but also quite consistent.
Conclusion
- The new SAA POC assay was able to reliably measure SAA in horses’ whole blood and plasma.
- Similar to previously validated tests, the new SAA POC assay proved to be a valuable tool in examining the inflammatory response in various clinical diseases in horses.
Cite This Article
APA
Karam B, Hines S, Skipper L, Pusterla N.
(2020).
Whole-Blood Validation of a New Point-of-care Equine Serum Amyloid A Assay.
J Equine Vet Sci, 94, 103222.
https://doi.org/10.1016/j.jevs.2020.103222 Publication
Researcher Affiliations
- The William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA.
- Veterinary Medical Research and Development, Pullman, WA.
- The William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, Davis, CA.
- Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, Davis, CA. Electronic address: npusterla@ucdavis.edu.
MeSH Terms
- Acute-Phase Proteins
- Animals
- Biological Assay / veterinary
- Horses
- Immunoturbidimetry / veterinary
- Point-of-Care Systems
- Serum Amyloid A Protein
Citations
This article has been cited 4 times.- Kromann S, Olsen RH, Bojesen AM, Jensen HE, Thøfner I. Assessment of automated assays for serum amyloid A, haptoglobin (PIT54) and basic biochemistry in broiler breeders experimentally infected with Escherichia coli. Vet Res 2022 Mar 21;53(1):25.
- Kiemle J, Hindenberg S, Bauer N, Roecken M. Comparison of a point-of-care serum amyloid A analyzer frequently used in equine practice with 2 turbidimetric immunoassays used in human and veterinary medicine. J Vet Diagn Invest 2022 Jan;34(1):42-53.
- Supianto M, Lee SH, Jhung SH, Mohammad HB, Vu HM, Kim MS, Song WY, Kim TY, Lee HJ. Fluorescent paper strip immunoassay with carbon nanodots@silica for determination of human serum amyloid A1. Mikrochim Acta 2021 Oct 18;188(11):386.
- Souto PC, Santos MR, Orozco AMO, Bento LD, Ramirez-Lopez CJ, Girardi FM, Machado JCA, de Oliveira LL, da Fonseca LA. Enzyme-Linked Immunosorbent Assay (ELISA) Development for Equine Serum Amyloid A (SAA) Determination Using Recombinant Proteins. Methods Protoc 2025 Apr 7;8(2).
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