X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites.
Abstract: A routine parentage test as part of a conservation program for Kiso horses identified a possible sex chromosome anomaly in a 7 months-old filly because of an aberrant result using LEX3, an X-linked marker. We then analyzed X-linked markers (LEX26, TKY38, and TKY270), Y-linked markers (Eca.YH12, Eca.YM2, Eca.YA16, and the sex-determining region Y gene), and an X/Y marker (Amelogenin gene). This analysis demonstrated that the filly had not inherited an X chromosome from her sire. A karyotyping analysis confirmed that the filly was 63,XO. As it was suspected that the horse would be sterile, we avoided using the horse as a broodmare; the information should also serve to prevent unnecessary conflict between owners transferring and receiving the horse.
Publication Date: 2018-11-26 PubMed ID: 30473577PubMed Central: PMC6361659DOI: 10.1292/jvms.18-0253Google Scholar: Lookup
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Summary
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The research paper discusses the discovery of an X monosomy, which is a sex chromosome anomaly, in the endangered Kiso horse breed, detected during a customary parentage test.
Initial Findings
- The researchers tested the parentage of Kiso horses as part of a routine conservation program.
- While performing the test, they came across a possible sex chromosome anomaly in a 7-month-old filly.
- The anomaly was identified through an irregular result obtained from a marker linked to the X chromosome, known as LEX3.
Further Analysis
- The research team proceeded to scrutinize the filly using additional X-linked markers – LEX26, TKY38, TKY270, and other Y-linked markers such as Eca.YH12, Eca.YM2, Eca.YA16, and a sex-determining region Y gene.
- A X/Y marker called the Amelogenin gene was also applied during the analysis.
- It was concluded from this comprehensive analysis that the filly had not inherited an X chromosome from her father (sire).
Karyotyping Analysis and Outcome
- The team conducted a karyotyping analysis, which confirmed that the filly was 63,XO. This means she had one chromosome missing from the normal pair in a female horse, indicating a condition known as X monosomy.
- It was suspected that due to this genetic condition the horse would be sterile, therefore the decision was taken not to use her as a broodmare.
- The information obtained was also deemed useful to avoid potential disputes between horse owners during transfer and receiving of horses.
Please note that maintaining the genetic diversity and quality of endangered species like the Kiso horse is paramount, thus random detection of such genetic flaws is important for effective conservation plans.
Cite This Article
APA
Gamo S, Tozaki T, Kakoi H, Hirota KI, Nakamura K, Nishii N, Alumunia J, Takasu M.
(2018).
X monosomy in the endangered Kiso horse breed detected by a parentage test using sex chromosome linked genes and microsatellites.
J Vet Med Sci, 81(1), 91-94.
https://doi.org/10.1292/jvms.18-0253 Publication
Researcher Affiliations
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
- Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
- Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
- Genetic Analysis Department, Laboratory of Racing Chemistry, 1731-2 Tsurutamachi, Utsunomiya, Tochigi 320-0851, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
- Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
- Education and Research Center for Food Animal Health (GeFAH), Gifu University, 1-1 Yanagido, Gifu, Gifu 501-1193, Japan.
MeSH Terms
- Animals
- Female
- Genetic Linkage
- Horse Diseases / diagnosis
- Horse Diseases / genetics
- Horses
- Inheritance Patterns
- Karyotyping / veterinary
- Microsatellite Repeats
- Monosomy / genetics
- Sex Chromosome Aberrations / veterinary
- X Chromosome
References
This article includes 15 references
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Citations
This article has been cited 2 times.- Bugno-Poniewierska M, Raudsepp T. Horse Clinical Cytogenetics: Recurrent Themes and Novel Findings. Animals (Basel) 2021 Mar 16;11(3).
- Laseca N, Anaya G, Peña Z, Pirosanto Y, Molina A, Demyda Peyrás S. Impaired Reproductive Function in Equines: From Genetics to Genomics. Animals (Basel) 2021 Feb 3;11(2).
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